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Citations for Active Motif :
51 - 100 of 1679 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... ubiquitinated or unmodified Clr4 proteins were mixed with Recombinant Mononucleosomes (H3.1, Active Motif 31467) or Recombinant Mononucleosomes H3K9me3 (H3.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... the complex was biotin-labeled via incubation with recombinant sortase A5 enzyme (Active Motif) and a custom tri-glycine biotin peptide (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: The recombinant Akt1 protein (cat. No 81154) was purchased from Active Motif (Carlsbad, CA).
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bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin samples were incubated for 5 min at 37°C followed by a second incubation for 10 min at 37°C with shaking at 1,000 rpm after the addition of 1:100 enzymatic working solution (Active Motif, ChIP-IT Enzymatic Kit ...
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bioRxiv - Genomics 2020Quote: ... 10 μg anti-Ser2-P (Active Motif, 3E10), 8 μL anti-V5 (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... 10 μg anti-Ser5-P (Active Motif, 3E8), 10 μg anti-Ser2-P (Active Motif ...
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bioRxiv - Genomics 2022Quote: ... 10 ng spike-in chromatin (D.melanogaster, Active motif) and 1× incubation buffer (0.15% SDS ...
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bioRxiv - Genomics 2021Quote: ... 0.5% Triton X-100) and 2 μL 5mC antibody (Active Motif 61479) were added ...
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bioRxiv - Cell Biology 2020Quote: ... to a depth of 33.2 – 48.0 x 106 read pairs (Active Motif). Alignment to human genome (hg19 build ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Genomics 2023Quote: ... were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used: H3K27ac (39685 – Active Motif); H3K9ac (C5B11-9549 – Cell Signalling Technology) ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Genomics 2021Quote: ... 10 ng of spike-in Drosophila chromatin (Active Motif) was added to each sample with 2 µg spike-in antibody (Active Motif) ...
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bioRxiv - Developmental Biology 2022Quote: ... or 10 μl anti-H3K9me3 antibody (Active Motif, # 39162) overnight at 4°C and then bound to 50 μl of Protein A magnetic beads (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human histone H3 (Active Motif, Cat. 61799), 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ug antibodies were added to the supernatant and incubated overnight at 4°C with gentle rotation (CTCF, ab70303; RAD21, ab992; H3K4me3, Millipore, 04-745; H3K27ac, Active Motif,39685.). Chromatin was pulled down by protein G Sepharose beads (#17061801 ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Bioengineering 2021Quote: ... Antigen retrieval was then followed by 3 washes in 1x PBS for 5 min each and blocking at 37°C for 30 min with MAXblock blocking buffer (Active Motif, #15252)6 ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 µg H3K27ac antibody (Active Motif #39133), 4 µg H3K4me1 antibody (Millipore 07-436) ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... Chromatin precipitation was performed using rabbit anti-human H3K4me3 IgG Ab (Active Motif) as described previously [48] ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif, Cat. 39040), and 1:500 dilution mouse anti-human β-actin (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: ... Following permeabilization in 0.1% Triton X-100 and blocking in MAXblock medium (Active Motif), the cells were stained 3 hours with antibodies against γH2AX (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... or 10 μL anti-RNA pol II (Active Motif 39097) conjugated to 50 μL Dynabeads Protein G overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin was subjected to 3 x 10 minute cycles of ultrasonic shearing using a PIXUL multi-sample sonicator (Matchstick Technologies Inc., Kirkland, WA, and Active Motif, Carlsbad, CA).(Bomsztyk et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif, Cat. No. 37480), stabilizing solution D (Active Motif ...
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bioRxiv - Molecular Biology 2021Quote: ... GST or GST-SET8 full-length fusion proteins were ADP-ribosylated using PARP1 recombinant enzyme (Active Motif # 81037) during 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... or 10 μL of H3K27Ac antibody (Active Motif #39133, lot: 16119013). Total volume of bead plus antibody was brought to 200 μL using binding buffer and were rotated at room temperature for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Biochemistry 2020Quote: ... a rabbit polyclonal raised against the N-terminal half of recombinant PARP1 (Active Motif Ab 2793257, 1:2000 dilution); anti-C-ter-PARP1 ...
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bioRxiv - Genetics 2019Quote: ... containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta (CEBPB ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit polyclonal anti-histone H2AX phosphor Ser139 (Active Motif, AR-0149-10) at 1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit polyclonal anti-histone H2AX phosphor Ser139 (Active Motif, AR-0149-10) at 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% Triton X-100) with 1µg of antibody (Mouse anti 5mC, Active Motif, 39649 / Mouse IgG) per 1µg of DNA in a 100µl volume ...
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bioRxiv - Molecular Biology 2020Quote: ... TET1 (Active Motif) and IgG as a control (Santa Cruz Biotechnology) ...
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bioRxiv - Genetics 2020Quote: ... CTCF (Active Motif), H3K4me1 (abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... from Active motif for DNMT1 (#39-906) ...