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Citations for Active Motif :
51 - 100 of 521 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and cytoplasmic fractions were extracted using the Nuclear Extract Kit (Active Motif; no. 40010) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear and cytoplasmic RNA separation assay was conducted using RNA Subcellular Isolation Kit (Active motif), according to previously detailed method(61) ...
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bioRxiv - Cancer Biology 2023Quote: ... and cytosolic extracts from NES cells were prepared by using the Nuclear Extract Kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The histone proteins and peptide used for antibody competition were histone H3K23me3 (Active motif, 31264), histone H3K9me3 (Active motif ...
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bioRxiv - Cell Biology 2023Quote: ... TransAM p65 NF-κB activation assay was performed using nuclear lysate as per standard protocol (Active Motif). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Cell Biology 2021Quote: Cytoplasm and nucleus of keratinocytes were separated by using Nuclear Extract Kit (Active Motif, Inc., Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Nrf2 activity was quantified using nuclear extracts and a TransAm NRF2 activity kit from Active Motif (cat# 50296) following the manufacturers included instructions.
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bioRxiv - Cell Biology 2024Quote: ... after which CTCF protein-RNA complexes were immunoprecipitated using the relevant antibody (anti-CTCF, Active Motif 61331). In parallel to the IP ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Microbiology 2024Quote: ... Protein was harvested and separated via SDS-PAGE to western blot against LexA (antibody purchased from Active Motif). Intensities of intact and cleaved LexA bands were normalized to a background band that was unaltered by treatment using FIJI.
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and whole-cell extracts were prepared from 1.5×107 cells per condition with and without DOX treatment for 4 d using the Nuclear Extract Kit (Active Motif). Co-immunoprecipitation was performed with the nuclear fraction using the Dynabeads Co-Immunoprecipitation Kit (Thermo Fisher ...
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bioRxiv - Physiology 2022Quote: ... The activity of NF-κB in binding DNA was assessed in nuclear fractions by a DNA Binding TransAM NF-κBp65 Assay (Active Motif).
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Genetics 2023Quote: ... The collected supernatants were incubated overnight with Protein A dynabeads (Life technology, 10001D) associated with H3K27Ac antibody (Active Motif). Next the input sample and chromatin-beads complexes sample were digested ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 mM ascorbic acid and 50 nM Chromeo 642 azide dye (Active Motif 15288) diluted in 1x PBS ...
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bioRxiv - Cancer Biology 2021Quote: The effect of CLEFMA on NF-κB p65-DNA interaction was assessed in the nuclear fraction of H441 and CCL151 cells by TransAM NF-κB p65 kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA, USA). A specific NF-κB inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 15 ug of purified rabbit polyclonal YAP-specific antibody (US Biologicals, Y1200-01D) or rabbit IgG (control) and protein G magnetic beads (Active Motif). Immunoprecipitated chromatin-protein complexes were washed extensively with RIPA buffer (1x ...
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bioRxiv - Genomics 2024Quote: ... End repair was carried out by adding 30 μl of a solution containing 0.5 mM biotin-14-dATP (Active Motif 14138), 0.5 mM biotin-14-dCTP (AAT bio 17019) ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
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bioRxiv - Neuroscience 2023Quote: ... myelin basic protein (Active Motif, 31314) was employed as a substrate for recombinant CDKL5 ...
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bioRxiv - Molecular Biology 2021Quote: ... the pellet resuspended in 147 μL of 2x SDS loading dye and DNA was sheared by sonication using the EpiShear Probe Sonicator (Active Motif). 3 μL β-mercaptoethanol was added and samples were heated to 95°C for 10 min before the same volume of each sample was loaded on NuPAGE Novex 4-12% Bis-Tris protein gels and run at 150 V ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8μM of purified DNMT1 protein (Active Motif) was incubated with 30000 cpm of 32P-labelled aptamers and single or double-stranded ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Immunology 2024Quote: Recombinant Tn5 transposase protein (Active Motif #81284) lots were assembled with IDT custom oligos mosaic end (ME ...
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bioRxiv - Genomics 2022Quote: ... 1 μg anti-H2Av antibody (spike-in antibody, Active Motif), 10 ng spike-in chromatin (D.melanogaster ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Developmental Biology 2023Quote: ... the rest was incubated with primary antibody (H3K4me3 antibody: Cell signaling Technology, 9727; H3K9me3 antibody: Active Motif, 39161) -coated Dynabeads Protein A/G for overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2024Quote: ... 30 μl protein-G magnetic beads (Active Motif) and 10 μg CTCF antibody (Active motif ...
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bioRxiv - Cancer Biology 2024Quote: ... primary antibody (Active Motif), secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant CDKL5 and myelin basic protein (Active Motif, 31314) as a substrate were incubated in a kinase buffer (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... 5 μg of antibody and 2 µg of spike-in antibody (Active Motif 61686) were bound to 50 μl protein A or protein G Dynabeads (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... H3K27Ac antibody (39133, Active Motif), or NEUROD1 antibody (sc-1084 ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies for H3K4me3 (Active Motif), p300 (Developmental Studies Hybridoma Bank) ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27ac antibody (Active Motif #39134), BRD4 antibody (Epicypher #13-2003 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibody (Active motif, 61686) were included to normalize SMC1A ChIP-seq ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... we prepared antibody solutions by diluting primary antibody in MAXbind Staining buffer (Active Motif, #15253) and performed post-decrowding immunostaining (Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5-1 μg of primary antibody with 0.25 μg Spike-in antibody (Active Motif 61686) diluted in Digitonin block buffer was added to the bead-nuclei mixture and incubated for 3 hours (histone marks ...
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CENP-C regulates centromere assembly, asymmetry and epigenetic age in Drosophila germline stem cellsbioRxiv - Cell Biology 2020Quote: ... the following antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:1000), sheep anti-CENP-C (Dattoli et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies were used for immunofluorescence: RNAPII CTD phospho-Tyr antibody (Active Motif, #61383), Phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant AGO (AGO1, AGO2, AGO3) protein was purchased from Active Motif (AGO1 Catalog No ...
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bioRxiv - Plant Biology 2021Quote: ... Histone H3K4me3 antibody (Active Motif: 39915) and anti-acetyl-Histone H3 antibody (Millipore ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... anti-H3K27me3 (Antibody 1: Active Motif, 39055 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-H2A antibody (Active Motif 91325), anti-H3K27me3 antibody (Millipore 07-449) ...