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Citations for Active Motif :
51 - 100 of 380 citations for 6 METHYL 2 OXO 1 2 DIHYDRO QUINOLINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif, #61686) overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μg of spikein antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was sonicated (300-700 bp size) and MIRA pull-down was performed using the Methyl Collector Ultra Kit (Active Motif), which uses a MBD2b/MBD3L1 protein complex ...
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bioRxiv - Immunology 2021Quote: ... and 6 μl 5M NaCL (Active Motif) for 30 minutes at 37°C followed by 2 μl proteinase K (Active Motif ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
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bioRxiv - Immunology 2023Quote: Approximately 2 million BMDCs were processed according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009) as previously described75 ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... Two μg of each peptide and 2 μg of purified wild type or mutant SET8 or full-length SETD2 (Active Motif) were incubated with radioactively labeled [3H] AdoMet at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 µg H3K27ac antibody (Active Motif #39133), 4 µg H3K4me1 antibody (Millipore 07-436) ...
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bioRxiv - Cell Biology 2022Quote: ... Histone 3 (H3) (Active Motif 39763), Histone H3K4me3 (Active Motif 39160) ...
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bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
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bioRxiv - Biophysics 2022Quote: ... and 3 μM Sortase A5 (Active Motif) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... (Histone 3 (Active Motif, cat no. 39763); GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μl of antibody (anti-H3K27Me3, Active Motif 39155, RRID: AB_2561020) was incubated with 100 μl of Dynabeads Protein G (Invitrogen 10004D ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Biophysics 2022Quote: H3K27ac (Active Motif, 39133 Lot 06921014 – Figure 3), biological replicates from Supplementary Figure SI-Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled to one of the following antibodies in the same buffer for 1 hr at room temperature with rotation: 3 μl H3 (Active motif 39763), 5 μl H3K4me3 (Millipore 04-745) ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 3 (Active Motif, #71023). For analysis ...
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bioRxiv - Cell Biology 2023Quote: 3’UTR reporter vectors were purchased from Active Motif, along with the miR-4787 mimic ...
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bioRxiv - Cancer Biology 2024Quote: ... MARK3 and 14-3-3ε were purchased (Active Motif, 81209 ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 mM ascorbic acid and 50 nM Chromeo 642 azide dye (Active Motif 15288) diluted in 1x PBS ...
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bioRxiv - Genetics 2020Quote: ... 3 μl of H3K27ac monoclonal antibody (Cat #39685, Active Motif) was added to each sample and rotated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: RelA and IRF-3 transcription factor activation assays (Active Motif) were carried out using the same protocol with the only difference being the 96-well plates and the antibodies used ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of anti-H3K4me3 antibodies (Active Motif, cat. No 39159) were added to the sonicated ChIP reaction and rotated vertically at 4° C overnight ...
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bioRxiv - Cancer Biology 2020Quote: SDHD 3’UTR LightSwitch Luciferase Reporter Assay was purchased from Active Motif. Cells were co-transfected with 30 nM miRNA and 100 ng reporter using Lipofectamine 3000 in a 96-well plate ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was sonicated for 3 minutes with a probe sonicator (Active Motif). Finally ...
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bioRxiv - Genomics 2023Quote: Site-specific mutagenesis was done on CCL2 LightSwitch 3′ UTR reporter (Active Motif) to generate constructs containing rs13900 C or rs13900 T alleles ...
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bioRxiv - Bioengineering 2019Quote: ... for 4-6 hours at room temperature and incubated in MAXbind Staining Medium (Active Motif, catalog no. 15251) containing primary antibodies at a concentration of 10 μg/mL overnight at 4□C ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were then washed 3 times with working Washing Solution (ChIP-IT, Active Motif) and then snap-frozen in liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitations were performed using 30 μg of cell line chromatin and 6 μg of N-Myc antibody (Active Motif # 61185) or 4 μg of H3K27Ac antibody (Active Motif #39133) ...
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 3 µg of rabbit anti-H3K9ac antibody (Active Motif; Carlsbad, CA) overnight at 4°C with rocking ...
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bioRxiv - Developmental Biology 2020Quote: ... then incubated 3 min with Stop Solution from the ChIP-IT High Sensitivity Kit (Active Motif) and washed three times with PBS before being frozen at −80°C until further use ...
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bioRxiv - Cell Biology 2020Quote: Histones were purified from cells by growing macrophages to a density of 3×107 (Active Motif - 40025). Histone H3K27me3 conversion was measured using the Active Motif kit (Epiquik Histone Methyltransferase activity assay kit (P-3005).
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bioRxiv - Developmental Biology 2019Quote: ... followed by 3 minutes incubation with Stop Solution from the ChIP-IT High sensitive kit (Active Motif). Samples were then washed 3 times with working Washing Solution (ChIP-IT ...
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bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 times with cold PBS and resuspended in ChIP buffer supplemented with PIC (Protease Inhibitor Cocktail; Active Motif) and PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Molecular Biology 2024Quote: ... full length of circASH1L was subcloned to the 3′UTR region of pLightSwitch backbone (Active Motif, La Hulpe, Belgium). For the PLS-cASH1L-mut plasmid ...
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bioRxiv - Immunology 2021Quote: ... 1:1000 (Active Motif #61647, aa 1-19); Histone H4 C-terminal ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Genetics 2023Quote: ... chromatin was sonicated for 8 minutes in Buffer 3 (20 s on, 30 s off, 25% amplitude) using an EpiShear probe sonicator (Active Motif).
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bioRxiv - Neuroscience 2023Quote: ... Drosophila chromatin (Active Motif #53083; 1 ng per 1 μg chromatin) was added to the samples ...
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bioRxiv - Molecular Biology 2023Quote: ... γH2AX (Active Motif # 39117; 1 mL per 1 mg DNA for CHIP); Senataxin (Novus biological # NB100-57542 ...
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bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3: 1:500, 39155, Active Motif; anti-RAD21 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse or rabbit anti-H3K27ac (1:250; 39085, Active Motif; 1:1000; ab4729, Abcam), rabbit anti-Lamin B1 (1:100 ...