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Citations for Active Motif :
51 - 100 of 376 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 3 µg of rabbit anti-H3K9ac antibody (Active Motif; Carlsbad, CA) overnight at 4°C with rocking ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with 4 µg of Pol II antibody (Active Motif, 4H8) at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... then incubated 3 min with Stop Solution from the ChIP-IT High Sensitivity Kit (Active Motif) and washed three times with PBS before being frozen at −80°C until further use ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no ...
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bioRxiv - Cell Biology 2020Quote: Histones were purified from cells by growing macrophages to a density of 3×107 (Active Motif - 40025). Histone H3K27me3 conversion was measured using the Active Motif kit (Epiquik Histone Methyltransferase activity assay kit (P-3005).
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bioRxiv - Developmental Biology 2019Quote: ... followed by 3 minutes incubation with Stop Solution from the ChIP-IT High sensitive kit (Active Motif). Samples were then washed 3 times with working Washing Solution (ChIP-IT ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (Histone H3 – Abcam ab1791, H3k27me3 – Active Motif – 39155 and TLR-4 -Abcam 76B357.1) were then diluted 1:1000 in blocking buffer and were incubated on a rocker overnight at 4C ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rotation at 4°C overnight with 2 ug of antibody (H3K27ac, #39133, Active Motif). Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2 ...
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bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21 ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 times with cold PBS and resuspended in ChIP buffer supplemented with PIC (Protease Inhibitor Cocktail; Active Motif) and PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Molecular Biology 2024Quote: ... full length of circASH1L was subcloned to the 3′UTR region of pLightSwitch backbone (Active Motif, La Hulpe, Belgium). For the PLS-cASH1L-mut plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... and genomic DNA regions of interest isolated using 4 μg of H3K27Ac antibody (Active Motif, cat# 39133). Complexes were washed ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif) overnight on an end-to-end rotator at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-H3.1/2 (Active Motif #61629; 1/250, 1/1000 and 1/4000) and anti-PTM (EMD Millipore #04-263 ...
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bioRxiv - Biophysics 2024Quote: ... anti-H3.1/2 (Active Motif #61629; 1/250, 1/1000 and 1/4000) and anti-PTM (EMD Millipore #04-263 ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif, #61686) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 1:1000 (Active Motif #61647, aa 1-19); Histone H4 C-terminal ...
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bioRxiv - Genetics 2023Quote: ... chromatin was sonicated for 8 minutes in Buffer 3 (20 s on, 30 s off, 25% amplitude) using an EpiShear probe sonicator (Active Motif).
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against Histone H3K27me3 (clone: 39155, Active Motif) Complexes were washed ...
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bioRxiv - Biochemistry 2019Quote: ... at 4°C with Active Motif enzymatic shearing cocktail in 100 µl Complete Digestion Buffer (both from Active Motif), and centrifuged for 10 min ...
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bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133 ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
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bioRxiv - Neuroscience 2023Quote: ... Drosophila chromatin (Active Motif #53083; 1 ng per 1 μg chromatin) was added to the samples ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Biophysics 2019Quote: ... and the mix was incubated overnight at 4 ºC with antibodies against MeCP2 (D4F3, Cell Signalling) plus spike-in antibody (Active Motif). For ChIP-seq library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... γH2AX (Active Motif # 39117; 1 mL per 1 mg DNA for CHIP); Senataxin (Novus biological # NB100-57542 ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ug antibodies were added to the supernatant and incubated overnight at 4°C with gentle rotation (CTCF, ab70303; RAD21, ab992; H3K4me3, Millipore, 04-745; H3K27ac, Active Motif,39685.). Chromatin was pulled down by protein G Sepharose beads (#17061801 ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 µg H3K27ac antibody (Active Motif #39133), 4 µg H3K4me1 antibody (Millipore 07-436) ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3 ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3: 1:500, 39155, Active Motif; anti-RAD21 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse or rabbit anti-H3K27ac (1:250; 39085, Active Motif; 1:1000; ab4729, Abcam), rabbit anti-Lamin B1 (1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... H3K27me3 (1:1000, Active Motif), and Actin (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... 1:15000 (Active motif #61277); rat anti-histone H3 N-terminal mAb ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:500 (39787, Active Motif), anti-Calnexin ...