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Citations for Active Motif :
851 - 900 of 1695 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ChIP was performed as previously described in duplicate for Flag-NLS-M.CvIPI-Lamin B1 cells treated with DMSO or GSK343 and induced with doxycycline as described above,70 with the following modifications: spike-in Drosophila S2 chromatin (Active Motif, 08221011) at a concentration of 10 ng/million cells and spike-in antibody (Active Motif ...
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bioRxiv - Genomics 2022Quote: ... KLF5 (61099, Active Motif), KLF9 (701888 ...
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bioRxiv - Genomics 2022Quote: ... histones were extracted according to the recommended manufacturer’s one-step protocol using the Histone Extraction Kit (Active Motif). The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... SUMO2 (Eisenhardt et al., 2015) and histone H1 (catalog # 81126) and H1.2 (catalog # 81252) from Active motif. All recombinant proteins are frozen in small single-use aliquots at the highest possible concentrations and stored at −80°C ...
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bioRxiv - Pathology 2022Quote: ... 7.35 nM PRMT5/MEP50 complex (Active motif, Cat#31356), 0.4 mM N-terminal H4 peptides ...
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bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... rabbit anti- pSTAT1-Ser727 (1:100, Active Motif #39634). pAG-MNase (prepared as in [61 ...
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bioRxiv - Genomics 2022Quote: ChIP was performed using ChIP-IT Express Chromatin Immunoprecipitation kits (Active Motif, 53009). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA regions of interest were isolated using antibodies against RelA (Active Motif, catalog number 39369; RRID:AB_2793231) in articular chondrocytes ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated and the DNA sheared to an average length of 300-500bp using the EpiShear™ Probe Sonicator (Active Motif, cat # 53051) with an EpiS-hear™ Cooled Sonication Platform (Active Motif ...
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bioRxiv - Genetics 2022Quote: ... A Global DNA Methylation Assay Kit (Cat#55017, Active Motif, USA) was used for DNA methylation analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... following well-established protocols and using a ChIP-validated H4K12ac antibody (Active Motif, #39165). Peaks were called using either the MACS (58 ...
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bioRxiv - Cancer Biology 2022Quote: Analyses of genome-wide H4K12ac mark in leukemic cells isolated from Sirt1 conditional knockout E-NOTCH1-induced T-ALL-bearing mice acutely treated as before with vehicle or tamoxifen to induce isogenic loss of Sirt1 was done by Active Motif, following well-established protocols and using a ChIP-validated H4K12ac antibody (Active Motif ...
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bioRxiv - Cancer Biology 2022Quote: ... we employed Active Motif ChIP-seq spike-in using Drosophila chromatin (Active Motif catalogue # 53083) and an antibody against Drosophila specific histone variant H2Av (Active Motif ...
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bioRxiv - Cancer Biology 2022Quote: ... and an antibody against Drosophila specific histone variant H2Av (Active Motif, catalogue # 61686) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ChIP-seq was performed using the ChIP-IT High Sensitivity kit (Active Motif, cat. 53040) as described previously (38) ...
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bioRxiv - Cell Biology 2022Quote: MODified histone peptide arrays (Active Motif) composed of 384 unique histone peptides representing acetylation ...
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bioRxiv - Cancer Biology 2022Quote: ... or Anti-H3K27ac (Active Motif, 39133) antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5-1 μg of primary antibody with 0.25 μg Spike-in antibody (Active Motif 61686) diluted in Digitonin block buffer was added to the bead-nuclei mixture and incubated for 3 hours (histone marks ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-H2A antibody (Active Motif 91325), anti-H3K27me3 antibody (Millipore 07-449) ...
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bioRxiv - Molecular Biology 2022Quote: ... H3K27ac (Active Motif 39133).
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bioRxiv - Microbiology 2022Quote: ... RNA-seq and data analysis was performed by Active Motif. In short ...
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bioRxiv - Cancer Biology 2022Quote: Ras GTPase Chemi ELISA Kit (Active Motif, 52097) was used to measure Ras GTPase level in organoids ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-AR (39781, Active Motif, Carlsbad, CA, USA) 1:1,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... were washed twice in 1X IP Low buffer supplemented with protease inhibitor and PMSF following manufacturer’s guidelines (Active Motif, 37511). 200µl of nuclear extracts were incubated with the GFP-Trap beads for 1hour at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear fraction digestion and collection was performed using Nuclear Complex Co-IP Kit (Active Motif, 54001). 25µl per IP of GFP-Trap Magnetic Agarose beads (Chromotek ...
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bioRxiv - Cell Biology 2022Quote: ... and H3K27ac (Active Motif, 39133) were used ...
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bioRxiv - Genomics 2022Quote: ... and 5 µg of anti-H3K27Ac (Active Motif, 39133) were added overnight at 4°C with rotation ...
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bioRxiv - Immunology 2022Quote: ... or 10 μL of H3K27Ac antibody (Active Motif #39133, lot: 16119013). Total volume of bead plus antibody was brought to 200 μL using binding buffer and were rotated at room temperature for 30 min ...
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bioRxiv - Immunology 2022Quote: ... and total H3 (Active Motif, 39763, 1:500). Samples were then stained with anti-mouse (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... H3K36me3 (Active Motif, 61021, 1:2000), and total H3 (Active Motif ...
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bioRxiv - Neuroscience 2022Quote: ... H3K27me3 (Active Motif 39157), DNMT1 (Active Motif 39204 ...
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bioRxiv - Neuroscience 2022Quote: ... DNMT1 (Active Motif 39204) and DNMT3A (Active Motif 39206) ...
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bioRxiv - Neuroscience 2022Quote: ... and DNMT3A (Active Motif 39206). Rabbit IgG were used as control for ChIP and primers within a gene desert on chromosome 16 were used as a negative control for qPCR.
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear and cytoplasmic fractionation was performed with Nuclear Extract Kit (40010, Active Motif) according to the manufacture’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and rabbit anti-H3K27ac (Active Motif: 39136). A total of 50 brains were collected for transcription factor samples and 25 brains were collected for histone mark samples ...
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bioRxiv - Developmental Biology 2022Quote: ... antibodies used were rabbit anti-H3K4me3 (Active Motif: 39159), rabbit anti-H3K27me3 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).
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bioRxiv - Developmental Biology 2022Quote: Making libraries and sequencing: Libraries were made as in ATAC-Seq kit (Active Motif, 53150) with some modifications ...
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bioRxiv - Genomics 2022Quote: ... H3K4me3 (Active Motif - Cat. No.39160), H3K79me2 (Active Motif - Cat ...
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bioRxiv - Genomics 2022Quote: ... Antibodies used: H3K36me3 (Active Motif - Cat. No.61102), H3K4me1 (Active Motif - Cat ...
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bioRxiv - Genomics 2022Quote: ... H3K4me1 (Active Motif - Cat. No.39298), H2K27ac (Active Motif - Cat ...
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bioRxiv - Genomics 2022Quote: ... H2K27ac (Active Motif - Cat. No.39134), H3K4me3 (Active Motif - Cat ...
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bioRxiv - Genomics 2022Quote: ... H3K79me2 (Active Motif - Cat. No.39144), H3K79me3 (Novus Biologicals - Cat ...
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bioRxiv - Genomics 2022Quote: Allele-specific and non-allele-specific data analysis was carried out using the data anlysis webtool provided by Active Motif® ...
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bioRxiv - Genomics 2022Quote: ... we used CUT&Tag-IT™ assay kit (Cat. No. 53160) from Active Motif® ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg H3K27ac (Active Motif, 39034), or 2 μg H3K27me3 (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...