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Citations for Active Motif :
801 - 850 of 850 citations for Mouse Anti HIV 1 p24 Recombinant Antibody clone 183 H12 5C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and phospho S2 polymerase II (Active Motif, 61083, 1:200), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit pan-acetyl H3 (Active Motif, Cat#61638, 1:1000), rabbit Total Histone H3 (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit pan-acetyl H4 (Active Motif, Cat#39925, 1:1000), rabbit Total Histone H4 (Cell Signaling Technology ...
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bioRxiv - Genomics 2024Quote: ... rabbit-H3K4Me3 (Active Motif; 39159, used at 1:500 dilution); rabbit-TrxR2 (Thermo Fisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2024Quote: ... For assessing histone modification 70 μg of chromatin from WT and KO cells was taken and 3ug antibodies were added H3K27ac (Active motif, #39134), H3K27me3 (Abcam ...
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bioRxiv - Genomics 2024Quote: ... Cell fractionations were verified by western blot as described in (Mayer and Churchman 2016) with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (ThermoFisher MA5-15738).
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bioRxiv - Genomics 2024Quote: ... were bound with antibodies of interests (H3K27ac, Active Motif 39133; H3K4me3, Active Motif 39915; H3K9me3, Abcam ab176916 or H3K27me3, Active Motif 39155) and incubated at 4°C for 36 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... using 18 μg of sheared chromatin from 30 pooled embryonic testes and 5 μl of COUP-TFII antibody (Active Motif, CA 61213). Libraries were sequenced by Active Motif as single-end 75-mers on an Illumina NextSeq 500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... was used to detect the construct and the anti-H3 C terminus (Active Motif, Cat#39052) as a control.
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-CTCF (Active Motif; Cat#: 61311) antibody overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... or 10 μl anti- H3K56ac for the early-S time point (Active Motif, 39281 (lot# 14013003). Immunoprecipitation ...
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... 1× potease inhibitors) and either 1µL of H3K27me3 (Active Motif #61017) or IgG (abcam #ab6709 ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit H3K27ac (1:500; Active Motif, 39133; Carlsbad, CA, USA). Two sets of cells were immunolabeled for the biomarkers ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatin immunoprecipitation was carried out using multiplexed MOWChIP assay (Zhu et al., 2019) with anti-H3K4me3 (39159, Active Motif) and anti-H3K27ac (39135 ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... ChIP was carried out using multiplexed MOWChIP-seq with MNase digestion for chromatin fragmentation and anti-H3K27ac (39135, Active Motif) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... The western blots of the chromatin-associated proteins were performed with anti-RNA pol II CTD phospho Tyr1 (Active Motif), anti-Rpb1 (manufactured in our laboratory ...
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bioRxiv - Molecular Biology 2023Quote: Global DNA methylation LINE-1 were measured by Active Motif (55017, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Genomics 2024Quote: For anti-CENP-A CUT&Tag library preparation was used the CUT&Tag-IT kit from Active Motif (Cat. No. 53160). Each experiment was performed for 500,000 fresh cells ...
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bioRxiv - Molecular Biology 2023Quote: One μg of histone H3 peptides with or without H3K4me2 (a.a. 1-21, Active Motif) were incubated with 1 μg of purified GST-PHF21A protein fragments in binding buffer (50 mM Tris/Cl at pH 7.5 ...
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bioRxiv - Genomics 2022Quote: ... 0.5 million G1-phase DLD-1 cells were processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain at least 107 reads ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... and carried out from OSCs according to Fabry and colleagues (Fabry et al., 2019) using commercially available rabbit polyclonal anti-H3K9me3 (Active Motif, cat # 39161) and rabbit polyclonal anti-H3K4me2 (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Neuroscience 2024Quote: ... organoids were mechanically dissociated in a 1 mL Dounce homogenizer with a loose pestle (cat#: 40401; Active Motif) by pipetting up and down approximately 10 times ...
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bioRxiv - Molecular Biology 2020Quote: ... Global % 5mC levels were assayed using a Global DNA Methylation LINE-1 Kit (55017; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
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bioRxiv - Cancer Biology 2021Quote: ... MM.1S cells were exposed to Dex or EtOH for 1 hour and fixed according to the manufacturer’s instructions (RIME Cell Fixation protocol, Active Motif). Analysis were performed by Active motif and resuslts are given as supplementary table (see Additional file 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). Immunoprecipitated material and input chromatin were submitted to the Genomics Core Facility (GCF ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). PCR primers were designed in the TSS region for selected genes (Additional file 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804; H3K27ac, Active Motif, 39133 ...
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bioRxiv - Neuroscience 2022Quote: ... centrifuged 500 g for 5 min and washed a final time with 1 mL 1X CUT&Tag wash buffer (from CUT&Tag IT Assay Kit, Active Motif, #53610) or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5 ...
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bioRxiv - Immunology 2024Quote: Approximately >1 million astrocytes were used by cell preparation according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009). Briefly ...
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bioRxiv - Genomics 2021Quote: ... The resulting sheared chromatin fragments in a size range between 200 to 500 base pairs were incubated with H3K27me3 (Millipore #07-473,1:1000; Active Motif #39160, 1:500) or H4K20me3 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...