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Citations for Active Motif :
701 - 745 of 745 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated for 1h at room temperature with anti-H3K56ac (Active motif, 39281) at 1:2000 dilution ...
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bioRxiv - Immunology 2023Quote: ... IgG (isotype control) or H4K20me1 samples: 2 µg of the anti-pol II (Active Motif) or 2 µg of isotype control (IgG ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were incubated overnight at 4oC with mouse anti-PLZF (0.2 μg/ml, Active Motif) primary antibody ...
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bioRxiv - Genomics 2024Quote: ... CUT&Tag was performed on 50,000 nuclei by using rabbit anti-H3K9me3 (39765, Active Motif) or H3K27ac and CUTANA CUT&Tag Kit (14-1101 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2024Quote: ... For assessing histone modification 70 μg of chromatin from WT and KO cells was taken and 3ug antibodies were added H3K27ac (Active motif, #39134), H3K27me3 (Abcam ...
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bioRxiv - Genomics 2024Quote: ... Cell fractionations were verified by western blot as described in (Mayer and Churchman 2016) with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (ThermoFisher MA5-15738).
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bioRxiv - Genomics 2024Quote: ... were bound with antibodies of interests (H3K27ac, Active Motif 39133; H3K4me3, Active Motif 39915; H3K9me3, Abcam ab176916 or H3K27me3, Active Motif 39155) and incubated at 4°C for 36 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... using 18 μg of sheared chromatin from 30 pooled embryonic testes and 5 μl of COUP-TFII antibody (Active Motif, CA 61213). Libraries were sequenced by Active Motif as single-end 75-mers on an Illumina NextSeq 500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... was used to detect the construct and the anti-H3 C terminus (Active Motif, Cat#39052) as a control.
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked in PBS BSA 1% and incubated with anti-5hmC (39769; Active Motif) or anti-ssDNA antibody (LS-C64821 ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-CTCF (Active Motif; Cat#: 61311) antibody overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... or 10 μl anti- H3K56ac for the early-S time point (Active Motif, 39281 (lot# 14013003). Immunoprecipitation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we incubated them with HRP-conjugated goat anti-mouse IgG (0.5 μg/ml, Active Motif, USA) at 25 ◦C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Genetics 2024Quote: ... followed by a 1 hour incubation with the appropriate primary antibody (H3K27me1; Active Motif #61015, H3K27me3, Millipore #07-449, H3K27M, Active Motif #61803) diluted in Abdil-Tx ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with anti-bodies specific to 5mC (Active motif # 39649; dilution factor 1:1. 000). To control loading ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatin immunoprecipitation was carried out using multiplexed MOWChIP assay (Zhu et al., 2019) with anti-H3K4me3 (39159, Active Motif) and anti-H3K27ac (39135 ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... ChIP was carried out using multiplexed MOWChIP-seq with MNase digestion for chromatin fragmentation and anti-H3K27ac (39135, Active Motif) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... The western blots of the chromatin-associated proteins were performed with anti-RNA pol II CTD phospho Tyr1 (Active Motif), anti-Rpb1 (manufactured in our laboratory ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The sonicated lysate was added to 20 µl Dynabeads Protein A with 1.5 ug anti-H3K27ac (Active Motif, #39685 Mouse Monoclonal) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Genomics 2024Quote: For anti-CENP-A CUT&Tag library preparation was used the CUT&Tag-IT kit from Active Motif (Cat. No. 53160). Each experiment was performed for 500,000 fresh cells ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... and carried out from OSCs according to Fabry and colleagues (Fabry et al., 2019) using commercially available rabbit polyclonal anti-H3K9me3 (Active Motif, cat # 39161) and rabbit polyclonal anti-H3K4me2 (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.