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Citations for Active Motif :
651 - 700 of 744 citations for Anti Histone H2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... gonads were incubated in Rabbit-anti-H3K23me3 (1:150, Active Motif, 61499) in blocking buffer at 4ºC overnight ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Monoclonal anti-H3 (1B1-B2, 1:5000) was purchased from Active Motif. Polyclonal anti-Myc (ab9106 ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-MEIS1/2/3 (0.5 µg/ml, 39795, Active Motif, RRID:AB_2750570), rabbit anti-DHRS3 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-dCENP-A for Kc167 cells (AB_2793749, 1:100, Active motif), rabbit anti-dCENP-A for ovaries (AB_2793320 ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was performed with 9 μg of anti-H4K8ac (Active Motif, 61103) per ChIP sample after preclearing chromatin with protein A-Dynabeads ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight with primary FOXG1 antibody detecting both FOXG1 monomers and dimers (Rabbit polyclonal FOXG1, Active Motif) diluted in 5% BSA in TBST (Tris buffered saline with 0.1% Tween-20) ...
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bioRxiv - Genetics 2020Quote: ... were pre-blocked in fresh blocking buffer (0.5% BSA in PBS) and incubated with 2.5 µL of polyclonal H3K9me3 antibody (AB_2532132, Active Motif) or Rabbit IgG (negative control ...
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bioRxiv - Molecular Biology 2019Quote: ... using 10mg of antibody abcam ab992 together with 40ng of Drosophila melanogaster spike-in chromatin (Active motif 53083) and spike-in antibody (Active motif 61686) ...
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bioRxiv - Molecular Biology 2021Quote: ... All antibodies used in this study have been previously reported as suitable for ChIP: H3K4me3 (39159, Active Motif), H3K27ac (39133 ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μg of spikein antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then incubated overnight at 4C with the primary antibodies in PBST (Active motif (2AG-6F12-H4) anti-H3K9me3 (1:100) ...
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bioRxiv - Genomics 2023Quote: ... incubated for 45 min at RT with primary antibody diluted in 1X PBS/1% BSA (H3K27me3, Active motif-61017 ...
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bioRxiv - Genomics 2020Quote: ... then incubated overnight with 5 μg of either anti-H3K4me3 (Active Motif, 39159), anti-H3K27me3 (Active Motif ...
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bioRxiv - Immunology 2020Quote: ... Chromatin precipitation was performed using rabbit anti-human H3K4me3 IgG Ab (Active Motif) as described previously [48] ...
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bioRxiv - Plant Biology 2022Quote: ... and rabbit anti-H2AT120ph (Active Motif, USA, cat. no. 61196, dilution 1:500).
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was immunoprecipitated using 8 μg anti-H3K27ac (Catalogue No: 39133; Active Motif), 10 μL anti-BRD4 (Bethyl Laboratories ...
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bioRxiv - Biophysics 2024Quote: ... anti-H3.1/2 (Active Motif #61629; 1/250, 1/1000 and 1/4000) and anti-PTM (EMD Millipore #04-263 ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-H3.1/2 (Active Motif #61629; 1/250, 1/1000 and 1/4000) and anti-PTM (EMD Millipore #04-263 ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
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bioRxiv - Genetics 2023Quote: ... The collected supernatants were incubated overnight with Protein A dynabeads (Life technology, 10001D) associated with H3K27Ac antibody (Active Motif). Next the input sample and chromatin-beads complexes sample were digested ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitations were performed using 30 μg of cell line chromatin and 6 μg of N-Myc antibody (Active Motif # 61185) or 4 μg of H3K27Ac antibody (Active Motif #39133) ...
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bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Developmental Biology 2022Quote: ... This DNA was then used in immunoprecipitation (IP) reactions using 5-Methylcytosine (5-mC) mouse monoclonal antibody (39649, Active Motif). Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step ...
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bioRxiv - Immunology 2019Quote: ... Protein-DNA complexes were immuno-precipitated using 3μg of H3K27ac antibody from the same lot for all ChIP experiments (Catalogue #39133; Active Motif) coupled to 50μl Protein G Dynabeads (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
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bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
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bioRxiv - Neuroscience 2022Quote: ChIP-seq libraries were constructed from FACS-enriched RGCs (100,000 cells for each library) using an H3K27me3 antibody (1:100, Active Motif) or normal rabbit IgG (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse or rabbit anti-H3K27ac (1:250; 39085, Active Motif; 1:1000; ab4729, Abcam), rabbit anti-Lamin B1 (1:100 ...
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bioRxiv - Genomics 2022Quote: ... Mixed chromatin was then subjected to ChIP with 5ug anti-panH3Kac (Active Motif, 39139) and 1ug of anti-H2Av (Active Motif ...
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bioRxiv - Genomics 2019Quote: ... Membranes were incubated in anti-5-hydroxymethylcytidine (Active Motif, mouse monoclonal, 59.1, 0.4ug/mL) for 3hr at RT in 5% non-fat dry milk in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Developmental Biology 2020Quote: ... ChIP-Seq of T7 and MED12 were done in the presence of Drosophila spike-in chromatin and antibody following the manufacturers’ protocol (Active Motif). ChIP-Seq library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...
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bioRxiv - Genomics 2022Quote: ... 0.25% NP-40) and incubated for 1 h with 10 μg/IP of mouse bridging antibody (Active Motif; 1 μg/μl) at 4°C with end-to-end rotation ...
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bioRxiv - Genomics 2023Quote: ... The membrane was then blocked in 5% skimmed milk in TBS containing 0.1% Tween 20 (TBST) for 1 h at room temperature followed by incubation with 1:10,000 dilution of 5hmC antibody (Active Motif, 39069) overnight at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated for 1h at room temperature with anti-H3K56ac (Active motif, 39281) at 1:2000 dilution ...