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Citations for Active Motif :
651 - 686 of 686 citations for Anti CLCN4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Cell Biology 2020Quote: ... was used to detect the construct and the anti-H3 C terminus (Active Motif, Cat#39052) as a control.
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked in PBS BSA 1% and incubated with anti-5hmC (39769; Active Motif) or anti-ssDNA antibody (LS-C64821 ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-CTCF (Active Motif; Cat#: 61311) antibody overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... or 10 μl anti- H3K56ac for the early-S time point (Active Motif, 39281 (lot# 14013003). Immunoprecipitation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we incubated them with HRP-conjugated goat anti-mouse IgG (0.5 μg/ml, Active Motif, USA) at 25 ◦C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with anti-bodies specific to 5mC (Active motif # 39649; dilution factor 1:1. 000). To control loading ...
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bioRxiv - Developmental Biology 2019Quote: ... Drosophila chromatin and anti-H2Av were used according to manufacturer’s instructions were followed (Active Motif, 53093 and 61686). For immunoprecipitation ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... the RevMAb Biosciences (clone RM362, at 1:300 dilution) and the mouse monoclonal recombinant AbFlex m6A antibody (rAb) from Active Motif (at 1:300 dilution). A mixture of reference DNA oligonucleotides that correspond to the position of the modified nucleotides was used to compare with antibody binding (the last base at the 5’ end of the oligonucleotide hybridizes with the modified base ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatin immunoprecipitation was carried out using multiplexed MOWChIP assay (Zhu et al., 2019) with anti-H3K4me3 (39159, Active Motif) and anti-H3K27ac (39135 ...
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bioRxiv - Neuroscience 2021Quote: ... ChIP was carried out using multiplexed MOWChIP-seq with MNase digestion for chromatin fragmentation and anti-H3K27ac (39135, Active Motif) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... The western blots of the chromatin-associated proteins were performed with anti-RNA pol II CTD phospho Tyr1 (Active Motif), anti-Rpb1 (manufactured in our laboratory ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The sonicated lysate was added to 20 µl Dynabeads Protein A with 1.5 ug anti-H3K27ac (Active Motif, #39685 Mouse Monoclonal) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... and carried out from OSCs according to Fabry and colleagues (Fabry et al., 2019) using commercially available rabbit polyclonal anti-H3K9me3 (Active Motif, cat # 39161) and rabbit polyclonal anti-H3K4me2 (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.