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Citations for Active Motif :
551 - 600 of 623 citations for Son of sevenless homolog 1 SOS1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... mouse H3K4me1 (1:100; Active Motif, 39635; Carlsbad, CA, USA); mouse H3K27me3 (1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse H3K27me3 (1:250; Active Motif, 61017; Carlsbad, CA, USA); and rabbit H3K27ac (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... we labeled samples with SRF (Active Motif, 1:100 dilution), but later we switched to Patched and Wingless (DSHB ...
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bioRxiv - Developmental Biology 2020Quote: ... and mouse negative control primer set 1 (#71011, Active Motif) were used for validating the ChIP assays (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and phospho S2 polymerase II (Active Motif, 61083, 1:200), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit pan-acetyl H3 (Active Motif, Cat#61638, 1:1000), rabbit Total Histone H3 (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit pan-acetyl H4 (Active Motif, Cat#39925, 1:1000), rabbit Total Histone H4 (Cell Signaling Technology ...
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bioRxiv - Genomics 2023Quote: ... with a rabbit anti-H3K27ac (1:100, Active Motif #39135). Libraries were prepared from the “pellet” fractions using the Takara ThruPLEX DNA-seq kit as described above ...
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bioRxiv - Genomics 2024Quote: ... rabbit-H3K4Me3 (Active Motif; 39159, used at 1:500 dilution); rabbit-TrxR2 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Relative 5hmC levels were quantified blotting 500 ng of gDNA on a nitrocellulose membrane with an anti-5hmC antibody (Active Motif, 39769) diluted 1/10000 followed by an anti-rabbit antibody coupled to horseradish peroxydase (Dutscher NA934 ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... For assessing histone modification 70 μg of chromatin from WT and KO cells was taken and 3ug antibodies were added H3K27ac (Active motif, #39134), H3K27me3 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Pre-cleared chromatin samples were incubated overnight at 4°C on a rotator with histone modification antibodies–either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam ...
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bioRxiv - Genomics 2024Quote: ... Cell fractionations were verified by western blot as described in (Mayer and Churchman 2016) with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (ThermoFisher MA5-15738).
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bioRxiv - Genomics 2024Quote: ... were bound with antibodies of interests (H3K27ac, Active Motif 39133; H3K4me3, Active Motif 39915; H3K9me3, Abcam ab176916 or H3K27me3, Active Motif 39155) and incubated at 4°C for 36 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... using 18 μg of sheared chromatin from 30 pooled embryonic testes and 5 μl of COUP-TFII antibody (Active Motif, CA 61213). Libraries were sequenced by Active Motif as single-end 75-mers on an Illumina NextSeq 500 instrument.
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Genomics 2020Quote: ... plus a mouse monoclonal anti-H3K9me3 (1:200; Active motif 39286). For whole-cell proteomics ...
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bioRxiv - Immunology 2021Quote: ... Rat anti-AID (1:500, Active Motif, Carlsbad, CA, USA, #39886); mouse anti-GCN5 ...
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bioRxiv - Genetics 2020Quote: ... 1× potease inhibitors) and either 1µL of H3K27me3 (Active Motif #61017) or IgG (abcam #ab6709 ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit H3K27ac (1:500; Active Motif, 39133; Carlsbad, CA, USA). Two sets of cells were immunolabeled for the biomarkers ...
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bioRxiv - Cell Biology 2024Quote: ... or anti-pSer5 CTD T4 (Active Motif, 61361, 1:200 dilution) for overnight at 4°C and Alexa-labeled secondary antibodies (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... and /or Anti H3K27me3 1:1000 (Active Motif Cat No. 39155), and/or Anti H3K4me1 1:1000 (Active Motif Cat No ...
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bioRxiv - Systems Biology 2023Quote: ... and/or Anti H3K4me1 1:1000 (Active Motif Cat No. 61633). Antibodies were detected using the following secondary antibodies for their appropriate hots ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-dCENP-A for ovaries (AB_2793320, 1:200; Active Motif) and mouse anti-H3K9me2 (ab1220 ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-histone H3-pT3 (1:100, Active Motif, cat# 39154), sheep polyclonal anti human-BUB1 antibody ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-histone H2A-pT120 (1:2000, Active motif, cat# 39391), mouse anti-human HEC1 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclei were isolated and incubated overnight with Concanavalin A beads and 1.3 µl of the primary anti-H3K27me3 or anti-H3K27Ac antibody (Active Motif 39156, 23254116-11; Active Motif 39135, 23061102-11) per reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... gonads were incubated in Rabbit-anti-H3K23me3 (1:150, Active Motif, 61499) in blocking buffer at 4ºC overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Monoclonal anti-H3 (1B1-B2, 1:5000) was purchased from Active Motif. Polyclonal anti-Myc (ab9106 ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human histone H3 (Active Motif, Cat. 61799), 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif ...
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bioRxiv - Cancer Biology 2023Quote: ... or anti-trimethyl-histone H3 (Lys9) (1:2000; Cat# 61013, Active Motif), anti-ubiquityl-PCNA (Lys164 ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-dCENP-A for Kc167 cells (AB_2793749, 1:100, Active motif), rabbit anti-dCENP-A for ovaries (AB_2793320 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...