Labshake search
Citations for Active Motif :
551 - 600 of 605 citations for Rat Insulin Like Growth Factor 1 IGF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... Tissues were stained with anti-acetylated histone H3 antibody (1:250; #39140, Active Motif, CA, USA) overnight at 4°C ...
-
bioRxiv - Systems Biology 2023Quote: ... Antibodies were used at the following concentrations: Anti H3K27ac 1:1000 (Active Motif Cat No. 39685), and /or Anti H3K27me3 1:1000 (Active Motif Cat No ...
-
bioRxiv - Immunology 2024Quote: ... and 1 µl of the primary anti-H3K27ac antibody per reaction (Active Motif, catalog number 39135). After incubation with the secondary anti-rabbit antibody (1:100) ...
-
bioRxiv - Plant Biology 2020Quote: Histone proteins were extracted either from in liquid grown seedlings or from leaf tissue with a Histone Purification Kit (Active Motif, Cat No. 40025) using manufacturing instruction with some modifications ...
-
bioRxiv - Molecular Biology 2020Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) (23) was performed from HeLa cells using the ChIP-IT High Sensitivity® kit from Active motif (ref #53040) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
-
bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
-
bioRxiv - Physiology 2022Quote: ... Chromatin preparations were subjected to ChIP using a ChIP-IT High Sensitivity Kit and Protein G Agarose Prepacked Columns (Active Motif, Carlsbad, CA, USA) using a PPARα (Abcam Ab24509 ...
-
bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
-
bioRxiv - Molecular Biology 2023Quote: CUT&Tag was performed as described previously (Kaya-Okur et al., 2020; Kaya-Okur et al., 2019) using the CUT&Tag-IT Assay Kit from Active Motif (Catalog No. 53160) and following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in 100 µl cold Antibody Buffer and 1 µl antibody (TET1, Active Motif #61943) was added for incubating on nutator overnight at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
-
bioRxiv - Developmental Biology 2022Quote: ... the following primary antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:500), sheep anti-CENP-C (Dattoli et al. ...
-
bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: We performed immunoprecipitations with 5 μg 1°Ab (Target: SATB2, Abcam, positive control: RNA pol II, Active Motif, negative control ...
-
bioRxiv - Genomics 2023Quote: ... incubated for 45 min at RT with primary antibody diluted in 1X PBS/1% BSA (H3K27me3, Active motif-61017 ...
-
bioRxiv - Neuroscience 2024Quote: ... organoids were mechanically dissociated in a 1 mL Dounce homogenizer with a loose pestle (cat#: 40401; Active Motif) by pipetting up and down approximately 10 times ...
-
bioRxiv - Cancer Biology 2020Quote: The DNA-binding activity of NF-κB was quantified by enzyme-linked immunosorbent assay using the TransAM® NF-κB family activation assay kit (Active Motif North America, Carlsbad, CA) to specifically detect and quantify the DNA-binding capacity of the NF-κB subunits p65 ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
-
bioRxiv - Developmental Biology 2019Quote: ... 5 µl was transformed into chemically competent TAM-1 cells in a 96-well plate format (Active Motif #11096) and selected for Chloramphenicol resistance ...
-
bioRxiv - Biochemistry 2020Quote: ... a rabbit polyclonal raised against the N-terminal half of recombinant PARP1 (Active Motif Ab 2793257, 1:2000 dilution); anti-C-ter-PARP1 ...
-
bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
-
bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... MM.1S cells were exposed to Dex or EtOH for 1 hour and fixed according to the manufacturer’s instructions (RIME Cell Fixation protocol, Active Motif). Analysis were performed by Active motif and resuslts are given as supplementary table (see Additional file 2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). Immunoprecipitated material and input chromatin were submitted to the Genomics Core Facility (GCF ...
-
bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). PCR primers were designed in the TSS region for selected genes (Additional file 1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Chromatin samples were incubated for 5 min at 37°C followed by a second incubation for 10 min at 37°C with shaking at 1,000 rpm after the addition of 1:100 enzymatic working solution (Active Motif, ChIP-IT Enzymatic Kit ...
-
bioRxiv - Neuroscience 2022Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
-
bioRxiv - Genomics 2021Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
-
bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041; H3K27Ac, Active Motif #39133 ...
-
bioRxiv - Neuroscience 2022Quote: ChIP-seq libraries were constructed from FACS-enriched RGCs (100,000 cells for each library) using an H3K27me3 antibody (1:100, Active Motif) or normal rabbit IgG (1:100 ...
-
bioRxiv - Developmental Biology 2021Quote: ... IP sample was incubated with 1 µg H3K4me3 antibody (in-house) (89) and 2 µg H3K27me3 antibody (Active Motif, Cat 61017) overnight with rotation at 4℃ ...
-
bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
-
bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
-
bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
-
bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with anti-5mC antibody (1:200 diluted in 0.5% goat serum + PBST, 39649, Active Motif, CA, USA) at 4 °C overnight and then washed three times with PBST for 5 min each ...
-
bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight with the following antibodies diluted at 1:2,500: anti-H3 (clone 1B1B2) (Cell Signalling Technology, #14269]) anti-H3K9ac (Active Motif, #39137) anti-H3K27ac [Active Motif ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
-
bioRxiv - Molecular Biology 2019Quote: ... and coupled to one of the following antibodies in the same buffer for 1 hr at room temperature with rotation: 3 μl H3 (Active motif 39763), 5 μl H3K4me3 (Millipore 04-745) ...
-
bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
-
bioRxiv - Genetics 2019Quote: ... Binding reactions with 3 × 105 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488) ...
-
bioRxiv - Immunology 2024Quote: Approximately >1 million astrocytes were used by cell preparation according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009). Briefly ...
-
bioRxiv - Genomics 2021Quote: ... The resulting sheared chromatin fragments in a size range between 200 to 500 base pairs were incubated with H3K27me3 (Millipore #07-473,1:1000; Active Motif #39160, 1:500) or H4K20me3 (Abcam ...