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Citations for Active Motif :
551 - 600 of 649 citations for Rat Histone Deacetylase 1 HDAC1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: PARP1 (1:1000, 39559, RRID: AB_2793257, Active Motif), Anti-poly-ADP-ribose binding reagent (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used in this paper were rabbit anti-CID (1:1000, Active motif), rabbit anti-SPC105R (1:4000 ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used: mouse anti-Srebp1 (39939, Active Motif, Carlsbad, CA, USA, dilution 1:1,000), rabbit anti-Srebp2 (112046 ...
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bioRxiv - Molecular Biology 2023Quote: Global DNA methylation LINE-1 were measured by Active Motif (55017, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was analyzed by ChIP-qPCR data relative to input using the mouse negative control and mouse positive control primer set Gapdh in the ChIP-IT qPCR analysis kit (Active Motif). Reaction conditions were programmed with initial denaturation at 50°C for 2 minutes and 95°C for 10 minutes followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: The binding of the p65 subunit of NFκB was quantified using the TransAM™ NFκB p65 kit (Active Motif, Carlsbad, CA), as we previously reported (Bai et al ...
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Epigenetic Changes in Saccharomyces cerevisiae Alters the Aromatic Profile in Alcoholic FermentationbioRxiv - Microbiology 2022Quote: The HDAC inhibition capacity of candidate epigenetic modifiers was measured by a fluorometric HDAC assay kit (Active Motif, Inc., CA, USA), following the manufacturer’s instructions with slight modification to suit the objectives of this research ...
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bioRxiv - Physiology 2022Quote: ... protein from a subset of muscles was fractionated into nuclear and cytoplasmic components using a Nuclear Extract Kit (Active Motif; 40010) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: PB NK cells were stimulated or not with IL-12 as previously described and Ch-IP was performed using ChIP-IT PBMCs Kit (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was sonicated (300-700 bp size) and MIRA pull-down was performed using the Methyl Collector Ultra Kit (Active Motif), which uses a MBD2b/MBD3L1 protein complex ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were carried out according to the manufacturer’s instructions with the Gelshift™ Chemiluminescent EMSA Kit (#37341, Active Motif, Waterloo, Belgium). Purified M35_S protein was diluted in storage buffer to reach the indicated concentration (0.1 to 10 µM ...
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bioRxiv - Neuroscience 2024Quote: Cells from proliferating iMOPs and iMOP-derived neurons were harvested for and processed using the CUT&Tag-IT Assay Kit (Active Motif) described by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Neuroscience 2019Quote: ... hiPSC-derived neurons were processed using the Active Motif ChIP-IT High Sensitivity kit according to the manufacturer’s recommendations (Active Motif, Cat. 53040). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: The ChIP was performed by using ChIP-IT® Express Kits according to the manufacturer’s instructions (Active Motif, San Diego, CA, USA). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Genetics 2022Quote: ... the nuclei suspension was diluted in a 1X volume of 1X Wash Buffer from a CUT&Tag-IT Assay Kit (Active Motif 53160). To estimate the yield of nuclei ...
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bioRxiv - Microbiology 2022Quote: ... parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples in the 300-500bp range were used for immunoprecipitation using the ChIP-IT High Sensitivity kit (Active Motif, cat. no. 53040). DNA was purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2023Quote: ... Reporters and mimics/inhibitors were reverse transfected with Lipofectamine 3000 (Fisher) and luminescence read after 48 hours with the LightSwitch™ Luciferase Assay Kit (Active Motif).
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bioRxiv - Developmental Biology 2019Quote: ... The primary antibodies used were as follows: H3K27me3 (mouse, 1:500, Active motif, cat. 61017), H3K27ac (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were used in IHC: PARP1 (1:100, 39559, RRID: AB_2793257, Active Motif; 1:100 ...
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bioRxiv - Immunology 2019Quote: ... Then beads were incubated with H3K27ac antibody (1 µg per IP, rabbit, Active Motif, 39133) or normal rabbit IgG (1 µg per IP ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5-1 μg of primary antibody with 0.25 μg Spike-in antibody (Active Motif 61686) diluted in Digitonin block buffer was added to the bead-nuclei mixture and incubated for 3 hours (histone marks ...
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bioRxiv - Genomics 2019Quote: ... transferred to PVDF membranes and probed with primary antibodies to BAF170 (Active Motif, 1:1000) and Histone H4 (1:6000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and H3K9ac antibody (Rep 1-1.4 ug or Rep 2-5 ug) (Active Motif, 39917). Enrichment was calculated relative to the geometric mean of two genes highly enriched for both H3K79me2 and H3K9ac in human (h ...
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bioRxiv - Genomics 2022Quote: ... 0.5 million G1-phase DLD-1 cells were processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain at least 107 reads ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated with primary anti-5mC antibody (1:200; Active Motif, Carlsbad, CA, USA) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... We used a rabbit anti-Cid/CENP-A antibody (Active Motif cat. 39713, 1:50) and rabbit anti-IgG as negative control (1:100) ...
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bioRxiv - Cancer Biology 2021Quote: The effect of CLEFMA on NF-κB p65-DNA interaction was assessed in the nuclear fraction of H441 and CCL151 cells by TransAM NF-κB p65 kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA, USA). A specific NF-κB inhibitor ...
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bioRxiv - Immunology 2022Quote: ChIP-qPCR analysis were performed using formaldehyde fixed purified BM pDCs with ChIP-IT® Express Chromatin Immunoprecipitation Kits (53035, Active Motif, USA) according to the manufacturer’s instructions ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... DNA binding capacity of the Nf-κB subunits relB and p52 were determined with the Nf-κB TransAM family kit (Active Motif, Rixensart, Belgium) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq)59 was performed from HeLa cells using the ChIP-IT High Sensitivity Kit from Active Motif (reference 53040) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were DNase treated and lysed in ice-cold ATAC seq lysis buffer from the ATAC-Seq Kit (Active Motif, Carlsbad, CA, USA) for 2 min ...
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bioRxiv - Bioengineering 2024Quote: Chromatin immunoprecipitation followed by real-time quantitative PCR (ChIP-qPCR) was performed using the ChIP-IT PBMC kit (53042; Active Motif, Carlsbad CA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked in PBS BSA 1% and incubated with anti-5hmC (39769; Active Motif) or anti-ssDNA antibody (LS-C64821 ...