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Citations for Vector Labs :
151 - 197 of 197 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... in calcium binding buffer (10mM HEPES, 150mM NaCl2, 2.5mM CaCl2, pH 7.4) containing 1x Carbo-Free Blocking Solution (Vector Laboratories, SP-5040) and protease inhibitor cocktail (11836145001 ...
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bioRxiv - Physiology 2021Quote: ... slides were placed in a blocking buffer (1:60 normal goat serum [Vector Laboratories; Cat. #S-1000] in 0.1% BSA/PBS) for 30 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed three times in wash buffer for 20 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs). Images were acquired using an upright Leica TCS SP8 confocal microscope with a 63X oil immersion objective lens (NA = 1.4 ...
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bioRxiv - Cell Biology 2022Quote: ... Finally,after washed with washing buffer and PBS for 3 times for 3 minute coverslips were mounted on Vectashield (Vector laboratories) for imaging ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Developmental Biology 2020Quote: ... antigen retrieval was performed by boiling slides in citrate-based or Tris-based antigen unmasking buffer (Vector Labs H3300 and H3301) for 45 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in 0.1M phosphate buffer and mounted on slides using Vectashield Antifade Mounting Medium with DAPI (H-1200, Vector Laboratories, USA) and images were visualized on a FV1200 confocal microscope (Olympus ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed for 45 minutes in wash buffer at RT and incubated in Vectashield anti-fade mounting medium (Vector Laboratories) adjusted for the objective refractive index for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed twice (wash buffer) at 37°C for 30 minutes each and mounted in VECTASHIELD with DAPI (Vector Labs). Stellaris RNA FISH probes against the full length Burdock consensus sequence were synthesized (LGC Biosearch technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... Fixative was washed out with a continuous flow of 50 μl blocking buffer (5% normal goat serum S1000 in low-salt CSFxB; Vector Labs), then reactions were incubated in that buffer for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2% Tween-20 in PHEM buffer and mounted in Vectashield (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Genetics 2022Quote: ... samples were washed two times in wash buffer for 30 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs).
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bioRxiv - Cell Biology 2023Quote: ... The blots were washed in TBST 3X followed by incubation in blocking buffer containing a goat anti-rabbit HRP tagged secondary antibody (Vector Labs) for 2 hr RT ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were washed three times in wash buffer for 20 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs). Images were acquired using a Leica Stellaris8 confocal microscope with a 63X oil immersion objective lens (NA = 1.4 ...
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bioRxiv - Genetics 2024Quote: ... samples were washed three times in the wash buffer for 20 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs). All solutions used for RNA FISH were RNase-free ...
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bioRxiv - Physiology 2024Quote: ... slides were placed in a blocking buffer (1:60 normal goat serum [Vector Laboratories; Cat. #S-1000] in 0.1% BSA/PBS) for 30 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... The wash buffer was finally wicked off and samples were washed twice with wash buffer and mounted with 12 µl VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200, Vector Laboratories). For AJM-1 antibody staining (MH27 ...
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bioRxiv - Developmental Biology 2020Quote: ... immunohistochemistry was carried out using heat-induced epitope retrieval buffers (A. Menarini) and Vectastain avidin-biotin-HRP reagents (Vector Lab PK-6100). Anti-BAP1 antibody (Cell signalling ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3x in 0.1M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed using sodium citrate buffer (pH 6.0) followed by quenching of endogenous peroxidases using BLOXALLTM blocking solution (Vector Laboratories, Inc., USA). Furthermore ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed three times with blocking buffer and coverslipped using Vectashield® Antifade Mounting Medium (Vector Laboratories, cat. number: H-1000). Images were acquired using a Leica TCS SP-8 confocal microscope and processed using ImageJ.
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bioRxiv - Neuroscience 2022Quote: ... Antigen retrieval was performed by microwaving the samples for 15 minutes in 0.01 M citrate buffer (pH = 6, Vector Laboratories, H-3300) in Millipore water ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in wash buffer and coverslipped with Vectashield hardset antifade mounting medium with a DAPI counterstain (Vector Laboratories, Burlingame, CA) and stored at 4°C.
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2 % Tween-20 in PHEM buffer and mounted using Vectashield mounting media (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Genetics 2024Quote: ... Cover slips were subsequently washed with Wash buffer B for 5 minutes and mounted onto the slides with Vectashield® Mounting Medium (H-1000, Vector Laboratories). Images were taken at the Imaging Facility ...
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bioRxiv - Neuroscience 2024Quote: Slices containing biocytin fills were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer overnight and processed with Vectastain ABC (avidin-biotinylated HRP complex) Elite Kit (Vector Labs, Burlingame, CA). The shape of the slice ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an additional step at 350W for 10 min in 10 mM citrate buffer at pH 6.0 (Vector Laboratories, Burlingame, CA, USA). Primary antibodies were diluted together in 5% normal goat serum/PBS and incubated ON at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... testes were washed 2 times for 30 min each at 37°C in the dark in the prewarmed wash buffer (2X SSC, 10% Formamide) and then resuspended in a drop of VECTASHIELD with DAPI (Vector Lab, H-1200).
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed again with washing buffer throughout the day and mounted with Vectashield Antifade Mounting Medium (Vector Laboratories Cat# H-1000-10) on a clear microscope slide ...
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bioRxiv - Microbiology 2019Quote: ... Epitope retrieval was performed by incubating slides with Vector Labs Antigen Unmasking Solution Citrate Buffer pH 6 (Vector labs, cat no. H-3300) for 20 min at 100°C in a steamer ...
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bioRxiv - Cell Biology 2021Quote: ... dipped in water to remove residual buffer and then mounted on glass slides using Vectashield anti-fade hard-set mounting medium containing DAPI (Vector Laboratories, H-1500). Slides were imaged using a Carl Zeiss LSM 710 confocal microscope and images were analysed using the Fiji distribution of ImageJ(33 ...
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Cell Biology 2022Quote: ... sections of formalin-fixed paraffin-embedded livers were subjected to antigen retrieval with Tris buffer PH=8.0 (Vector Labs H-3301; Newark CA) in a pressure cooker ...
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bioRxiv - Genetics 2022Quote: ... Wax sections were stained with hematoxylin and eosin for histological analysis and indirect immunofluorescence was performed after heat-induced epitope retrieval with citrate buffer (Vector Laboratories, H-3300). Confocal images were taken using a Zeiss LSM 880 microscope ...
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bioRxiv - Immunology 2023Quote: ... The secondary antibodies were washed off with above-mentioned washing buffer for 15 min and finally slides were mounted with DAPI (Vector Laboratories, Burlingame, USA). The stained cells were examined under a Zeiss LSM 780 fluorescence microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times in PBS and 2 times in 0.1 M phosphate buffer solution (10 min/wash) before being mounted and cover-slipped using Vectashield Vibrance w/DAPI (Vector Laboratories H-1800). Fluorescent conjugates of streptavidin were stored and prepared as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 × 5 min in TBS buffer and then incubated for 100 min in a solution of streptavidin Dylight 649 (Vector laboratories, SA5649, 1:100) in TBS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... or goat anti-mouse IgG Alexa Fluor 647 (Thermo, A32728) were diluted 1:200 in blocking buffer and 1% normal goat serum (Vector Laboratories, S-1000-20) and incubated for 2 hours at room temperature in a dark container ...
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bioRxiv - Cell Biology 2022Quote: ... EU detection was performed as indicated by the manufacturer and oocytes were then washed twice in Click-iT reaction rinse buffer and mounted on slide wells filled with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories Ref. H-1200).
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bioRxiv - Cell Biology 2023Quote: ... cells stained with DAPI followed by wash in 2x SSC buffer before being mounted using VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories H-1700-2).
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bioRxiv - Neuroscience 2023Quote: ... hBO sections were washed once with 1x PBS and blocked in blocking buffer containing 10% normal goat serum (NGS, Vector labs: S-1000-20), 0.1% BSA and 0.3% Triton-X in 1× PBS for one hour at RT ...
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bioRxiv - Neuroscience 2019Quote: ... After overnight incubation in primary antibody,the sections were rinsed in washing buffer and then incubated for 1 h in an anti-rabbit biotinylated antibody (Vector Laboratories, #ba-9400; 1:200) in washing buffer containing 2% normal goat serum ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed three times in wash buffer for 20 minutes each at 37°C and mounted in Vectashield with DAPI (Vector Laboratory, H-1200, Burlingame, USA). Images were acquired using an upright Leica Stellaris 8 confocal microscope with a 63X oil immersion objective lens (NA = 1.4 ...
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bioRxiv - Biochemistry 2023Quote: ... antigen retrieval was performed on the slides by incubating them in a steamer for 40 mins in citrate buffer (Vector Labs, Cat# H-3300-250). The slides were washed in water and then blocked using 5% goat serum in PBS for 1 hr at room temperature prior to adding primary antibodies diluted in the blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed twice with wash buffer for 30 minutes each at 37°C and mounted in Vectashield with DAPI (Vector Laboratory, H-1200, Burlingame, USA). Images were acquired using an upright Leica Stellaris 8 confocal microscope with a 63X oil immersion objective lens (NA = 1.4 ...