Labshake search
Citations for Vector Labs :
351 - 400 of 409 citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... Incubation in the primary antibodies was followed by 2-hour incubation in anti-rabbit secondary biotinylated antibodies (1:200, Vector Laboratories, Burlingame, CA), and then in avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Neuroscience 2019Quote: ... TC neurons in 250 micron brain slices or RGCs in flat-mount retinas were loaded by passive diffusion with 2% neurobiotin (Vector Labs, Burlingame, CA) and/or 100 μM CF568 dye (Biotium ...
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bioRxiv - Genetics 2021Quote: ... the gonads were again washed three times with PBSTw and resuspended in 1 drop of Vectashield containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA). The gonad was finally mounted on pads of 5% agarose in water on microscope slides and covered with a cover glass (Sulston and Horvitz ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in a 1/500 dilution of biotinylated mouse-anti-rabbit (Vector Laboratories, Burlingame, California) secondary antibody in diluent containing 2% NGS ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies listed in Table 2 were detected using ImmPress HRP Universal antibody polymer detection kit (Horse Anti-mouse/rabbit IgG, Vector Labs, MP-7500), as previously described for mouse in50 ...
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bioRxiv - Neuroscience 2021Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2021Quote: ... The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories, CA, USA). Cell counting was done manually on brain sections containing the mPFC ...
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bioRxiv - Molecular Biology 2019Quote: ... DAB staining was developed under a light microscope for between 2-10 min as per the manufacturer’s instructions with the ImmPACT DAB peroxidase substrate kit (Vector Labs, Cat# SK-4105). Slides were then counterstained with Hematoxylin Solution ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 μl/ml avidin and 6 μl/ml biotin; 2 hours at room temperature; Vector Laboratories, CA, USA), washed in PB ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated in the second antibody solution for 1 h at 37° C and subsequently mounted in Vectashield mounting medium containing 4′,6-diamidin-2-phenylindol (DAPI, Vector Laboratories, Burlingame, California) in a manner that conserves the 3D structure of the specimen [42] ...
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bioRxiv - Microbiology 2020Quote: ... Cells were counterstained with VECTASHIELD® Antifade Mounting Medium containing 1.5 µg ml- 1 4,6-diamidino-2-phenylindole (Vector Laboratories, Burlingame, CA, USA). Images were captured on a BX61 microscope equipped with a DP80 camera (Olympus Australia ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated with the sections for one hour before washing using PBS and mounting using Vectashield Antifade Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2022Quote: ... Cells incubated with MitoSOX™ were then fixed with 3.7% paraformaldehyde and applied with an anti-fading mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Live or fixed cell images were captured at an excitation/emission (in nm ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were mounted onto gelatinized slides and left to dry (at least 2 hours) before being coverslipped with VectaShield (Vector Laboratories, Burlingame, CA). The sections were imaged with 10x objective and 10x eyepiece magnification on a Leica LSI 3000 microscope ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Neuroscience 2023Quote: ... for 40 seconds and sections were finally coverslipped with Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) to label the nucleus (Vector Laboratories, H-1800). Slides were kept at 4 °C until imaging ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed with 0.1 M TBS (5 × 5) and the stain was allowed to develop for 2 minutes using an ImmPACT novaRED Substrate Kit (Vector Laboratories, SK-4805). Tissue sections were then washed for 5 min in tap water ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit IgG or Alexa Fluor 546 goat anti-rabbit IgG conjugates for 2 hours and mounted in VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Fluorescence images were taken using an inverted Nikon Eclipse TE2000-U microscope ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
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bioRxiv - Physiology 2023Quote: ... the sections were mounted with hard-set DAPI (4′, 6-diamidino-2-phenylindole –hard set; Cat# H-1500, Vector Laboratories Ltd., Peterborough, UK). The slides were left overnight in the dark at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 2 × 30s in 1M tris-HCl solution and then incubated in a DAB reaction solution (Vector Labs, SK-4100) for 5-15 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.4 containing 0.1% Tween 20) and squashed on a glass slide with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Newark, California, USA). Observations were performed using an Olympus BX61 microscope (N = 45-193 individuals per strain).
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bioRxiv - Physiology 2023Quote: ... The coverslips were washed in PBS before being mounted onto a microscope slide with 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield, Vector Laboratories, Burlingame, CA) mounting medium.
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bioRxiv - Neuroscience 2023Quote: The procedure was similar to that of single-cell recordings except that the micropipette was filled with 2% Neurobiotin (SP-1120, Vector Laboratories, CA., USA) in 0.5 M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were then incubated over night at 4°C on rotating wheel (or 2 h at RT) with a secondary antibody coupled to a fluorescent dye (DyLight® 549 (VECTOR lab # DI 1549) (1:400 ...
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bioRxiv - Cell Biology 2019Quote: ... oocytes were incubated with 4’,6-diamidino-2-phenylindole (DAPI) for 20 min and mounted onto glass slides using Vectashield (Vector Laboratories, Burlingame, CA, USA). Fluorescence was examined by confocal laser scanning microscopy (TCS SPE II ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were then destained by a gradient of ethanol plus a 2% hydrochloride wash and blocked with extra avidin/biotin (Vector Laboratories, Burlingame, CA, USA) and a Fab fragment block (Jackson Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... The histological sections were washed three times (10 min/each) with PBS, counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, 1:16000) and mounted with Vecta-Shield (H1000, Vector Laboratories, Burlingame, CA, USA) (Varman et al. ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 4-6 times over 2 hours in PBT at room temperature and mounted with VECTASHIELD Mounting Media (Vector Labs, Burlingame, CA, USA) using glass slides between two bridge glass coverslips ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The slides were then destained by a gradient of ethanol plus a 2% hydrochloride wash and blocked with extra avidin/biotin (Vector Laboratories, Burlingame, CA, USA) and a Fab fragment block (Jackson Laboratory ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each slide was coverslipped using a mounting medium that contained the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA) and stored at −20°C until image acquisition occurred.
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bioRxiv - Cancer Biology 2019Quote: ... Antigen retrieval was performed by heating slides to 93 ± 2 °C for 20 min in high-pH antigen unmasking solution (H-3301, Vector Labs, Burlingame, CA, USA), followed by blocking in 5% bovine serum albumin (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Bioengineering 2023Quote: ... Migrated cells located at the bottom side of the transwell membrane were permeabilized with 0.1% Triton X-100 in PBS and the transwell membrane was coverslipped with mounting medium containing DAPI (Vector Labs, Cat # H-1800-2). For each transwell ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... cells stained with DAPI followed by wash in 2x SSC buffer before being mounted using VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories H-1700-2).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was incubated at least 2 hr with 30 μl package volume of Wheat-germ (WGA) -conjugated agarose beads (Vector Laboratories, Inc., Burlingame, CA) on a rotating mixer at 4°C and washed 3 times with lysis buffer ...
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bioRxiv - Genetics 2024Quote: ... or Cy5 (1:1000; USBIO 518482) conjugated Lotus Tetragonolobus Lectin (LTA) and Rhodamine conjugated Dolichos Biflorus Agglutinin (DBA) (1:1000; Vector Laboratories RL-1032-2), was added to co-stain proximal tubules and collecting ducts ...
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bioRxiv - Synthetic Biology 2024Quote: ... glasses with the labeled cells were placed onto the Superfrost Plus adhesion slides (Epredia, EPBRSF41296SP) in 20 µl VECTASHIELD Vibrance Antifade Mounting Media (Vector Laboratories, H-1700-2) and stored at +4℃ in the dark.
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bioRxiv - Microbiology 2023Quote: ... Parasites were then stained with an anti-VSG-2 monoclonal antibody (Hovel-Miner et al., 2012) conjugated to Alexa647 and mounted in Vectashield (Vector Laboratories, H-1800-10) containing 4′,6-diamidino-2-phenylindole (DAPI) ...