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Citations for Vector Labs :
251 - 300 of 350 citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed an additional 3 × 5 minutes with PBS before adding mounting medium containing DAPI counterstain (Vector labs, H-1200) and applying glass coverslips ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were washed three times in PBTx for 15 min followed by five 5-minute washes in PBS and mounted in Vectashield antifade mounting medium containing DAPI (Vector Laboratories). Samples were imaged on an Olympus FV3000 Confocal Laser Scanning Microscope and processed in Imaris.
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea) simplicifolia lectin 1 (5 μg/ml) (Vector Laboratories; L-1100). OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα ...
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bioRxiv - Neuroscience 2023Quote: ... The CST was labeled using the Avidin Biotin Complex method (ABC) to detect the BDA by incubating in ABC (5 µL/mL A and B; PK-6100; Vector Laboratories) overnight at room temperature and co-labeled against GFAP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were washed twice in PBS (5 min) and mounted using Vectashield without DAPI (Vector Laboratories Inc., Burlingame, CA, USA) for immediate visualization by microscopy.
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 immunohistochemistry (IHC) was performed on 5 μm sections using a mouse on mouse immunodection kit (Vector Laboratories BMK-2202). Briefly ...
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bioRxiv - Pathology 2023Quote: ... The sections were washed with PBST (3x, 5 min) and developed using a DAB substrate kit (Vector Laboratories, Burlingame, CA, USA). Counterstaining was performed with hematoxylin ...
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bioRxiv - Biochemistry 2022Quote: ... Fixative was washed out with a continuous flow of 50 μl blocking buffer (5% normal goat serum S1000 in low-salt CSFxB; Vector Labs), then reactions were incubated in that buffer for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Organs were washed three times in PBS for 5 min per wash and mounted in Vectashield (Cat# H-1000, Vector Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... prior to IHC sections were rehydrated and an antigen retrieval step (Boiling 3 x 5 mins in Antigen Unmasking Solution, Vector Labs) was performed prior to blocking ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the fixed specimens were washed thrice for 5 min in 1x phosphate buffered saline (1xPBS; pH 7.4) and mounted using Vectashield mounting medium (H-1000, Vector Laboratories, CA). FITC-albumin and 10 kD dextran specimens were imaged using a ZEISS LSM900 confocal microscope with ZEISS Zen acquisition software (blue edition ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and mounted onto glass slides with DAPI nuclei stain VECTASHIELD mountant (Vector Laboratories). Samples were visualised on Evos Cell imaging system (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 0.5 μg/mL DAPI in PBS for 5 min prior to drying with ethanol and were mounted using Vectashield mounting medium without DAPI (Vector Laboratories). Images were acquired and analysed using a Leica fluorescence microscope ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained at room temperature with 5 μg/mL fluorescein-conjugated wheat-germ agglutinin (WGA, Vector Laboratories catalog no. FL-1021) for 15 minutes to detect exposed chitin or 50 μg/mL fluorescein-conjugated concanavalin A (ConA ...
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bioRxiv - Neuroscience 2021Quote: Free-floating parallel brain sections were washed with PBS + 0.5% Triton X-100 (PBS-T) and then blocked with 5% normal goat serum (NGS, #S1000, Vector laboratories, Burlingame, CA) in PBS-T for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Antibodies were as follows ...
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Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicitybioRxiv - Neuroscience 2019Quote: ... the brains were rinsed 5 × 10 mins with PBST and mounted on glass slides using Vectashield (Vector Laboratories INC., Burlingame, CA, USA) mounting medium.
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed 3×5 min in PBS and incubated with 1:1000 anti-rabbit biotinylated secondary antibody (BA-1000, Vector Laboratories, USA) in 1% NGS in PBST for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... The peroxidase reaction was produced by incubating the sections for 5 to 12 minutes in a DAB substrate solution (SK-4100; Vector Labs, CA). Reacted tissue was then mounted on gelatin coated slides ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed for 3 x 5 minutes in 0.1 M PBS and mounted on glass slides using Vectashield medium (containing DAPI as required, Vector Labs, Peterborough, UK) and cover-slipped.
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bioRxiv - Cell Biology 2020Quote: ... DAPI was applied as a nuclear counter stain at 0.3 μM for 5 minutes prior to mounting with medium (Vectashield soft, Vector Labs, Peterborough, UK) and placing the coverslips upside down onto microscope slides ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again (3 times in PBS/Tween, 5 minutes each) and incubated in biotinylated goat anti-rabbit (1: 200, Vector Laboratories, USA) or biotinylated horse anti-mouse (1 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times for 5 minutes with PBS and mounted using VECTASHIELD Antifade Mounting Medium (Vector Laboratories, H-1000) or Prolong Gold Antifade Mountant with DAPI (Thermo Fisher ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 5 times again and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat. # H-1400). Thalamocortical slice imaging was performed on an Andor Dragonfly microscope described below.
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bioRxiv - Neuroscience 2019Quote: ... followed by blocking at room temperature for 30 minutes in blocking solution containing 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA), 0.1% Triton X100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Anti-mouse monoclonal pS202/pT205 1:1000 (AT8 ...
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bioRxiv - Neuroscience 2022Quote: ... free-floating sections were washed with PBS 3 times and blocked in 5% normal goat serum (NGS, Vector Laboratories, S-1000-20) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed twice in PBS for 5 min and mounted on a drop of VECTASHIELD solution (Vector Laboratories, H-1200-10). Images were acquired using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Genetics 2024Quote: ... Cover slips were subsequently washed with Wash buffer B for 5 minutes and mounted onto the slides with Vectashield® Mounting Medium (H-1000, Vector Laboratories). Images were taken at the Imaging Facility ...
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bioRxiv - Microbiology 2024Quote: ... the specimens were washed in PBS for 5 min each and cover with glass slides using mounting medium (Vector Laboratories, United States). For imaging ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...