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Citations for Vector Labs :
701 - 750 of 788 citations for Recombinant Rabbit IL5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS (5 × 5 min) and incubated in biotinylated anti-rabbit secondary antibody (1:1000 in PBS with 0.1% bovine serum albumin, Vector Laboratories, Inc., Burlingame, CA) for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Immunohistochemical staining of tissue sections with no anti-mouse IgG antibody and the use of anti-Rabbit IgG antibody (Vector Laboratories, Burlingame, CA) were used for negative control analysis (data not shown) ...
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bioRxiv - Neuroscience 2019Quote: ... Following PBS-TX washes the sections were incubated with the secondary antibody (1:200 dilution; goat anti-rabbit DyLight 594; Vector Laboratories; Peterborough, UK), washed in PBS ...
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bioRxiv - Pathology 2020Quote: ... slides were rinsed in PBS and incubated in a horse anti-mouse/rabbit biotinylated IgG secondary antibody (VECTASTAIN Elite ABC Kit, Vector Labs, PK-6200). Sections were rinsed again ...
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bioRxiv - Neuroscience 2020Quote: ... in 2% BSA and 0.1 M TBS for 18 h at room temperature followed by incubation with biotinylated goat anti-rabbit Immunoglobin G secondary antibody (1:200; Vector Laboratories, Burlingame, CA) in 2% BSA and 0.1 M TBS for 75 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Reactive sites were identified with biotinylated antimouse and anti-rabbit secondary antibodies and peroxidase ABC reagents (Vector-Elite, Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s instructions and peroxidase activity was visualized with DAB+ reagents (DakoCytomation) ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times with 0.1% PBST and then incubated for 1 hour at room temperature with ImmPRESS anti-rabbit peroxidase conjugated antibody (Vector Laboratories, Burlingame, CA, USA). Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100) ...
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bioRxiv - Physiology 2020Quote: ... The slides were then incubated for 1 hour in a humidity chamber in a mixture of goat anti-rabbit peroxidase conjugated (Vector Labs PI-1000). and goat anti-chicken Alexafluor 488 conjugated (Abcam #ab150169) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with DyLight 594-conjugated goat anti-rabbit secondary antibody (1:1000, Vector Laboratories DI-1594) for 2 h on a shaker at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with biotinylated goat anti-rabbit secondary antibody (1:600 dilution; Vector Laboratories, catalog #BA-1000) in PBS-Tx and 1% NGS for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... All rat primary antibodies were incubated for either 20 or 30 minutes followed by biotinylated rabbit anti-rat linker antibody (BA-4001, Vector Labs, Burlingame, CA) applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800 ...
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bioRxiv - Cell Biology 2021Quote: ... or immunostained with rabbit anti-mouse polyclonal Ror2 (provided by Dr. Yasuhiro Minami, Kobe University) with DAB visualization (Vector Laboratories, Cat #SK-4100), and counterstained with hematoxylin and eosin (Sigma Aldrich ...
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bioRxiv - Pathology 2021Quote: ... we performed immunohistochemical staining following the avidin-biotin complex method using the VECTASTAIN Elite ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, CA, USA). The tissue sections were deparaffinized with xylene and ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... and sections were blocked with 1.5% normal serum (PK-6102, horse serum, mouse IgG or PK-6101, goat serum rabbit IgG; Vector Laboratories, Burlingame, CA, USA) for 1 h followed by incubation overnight at 4°C with anti-VEGF monoclonal antibody (SC-7269 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS (5 x 5 min) followed by a 1 hour incubation in biotinylated goat anti-rabbit secondary (1:500 in PBS, Vector Laboratories, BA-1000). Sections were rinsed in PBS (5 x 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated first with biotinylated goat anti-rabbit secondary antibody and then with avidin-biotin complex using the Elite Vectastain kit (Vector Laboratories, PK-6101). Chromogen reactions were performed with diaminobenzidine (SIGMAFAST DAB ...
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bioRxiv - Microbiology 2022Quote: ... brain sections were stained with rabbit anti-SINV capsid antibody (1:5000 dilution) and the Vectastain Elite ABC HRP kit (Vector Laboratories, PK-6101) was used for signal detection ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Pathology 2023Quote: ... 20 Sections were processed following ImmPRESS HRP Universal Antibody (horse anti-mouse/rabbit IgG) Polymer Detection Kit and ImmPACT DAB EqV Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30 min at RT with secondary antibody fluorochrome-conjugated anti-Rabbit Dylight 488 (Vector Laboratories, Newark, CA, USA). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated in anti-Mouse IgG or anti-Rabbit IgG secondary antibodies conjugated to Horse Radish Peroxidase (ImmPACT DAB, Vector Laboratories, Newark, CA) in a humid chamber for 30 min at 24L ...
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Bound integrins were quantified by an enzyme-linked immunosorbent (ELISA)-like solid-phase assay using biotinylated rabbit anti-velcro (against ACIS/BASE coiled-coil) antibody and HRP-conjugated streptavidin (VECTOR Laboratories #SA-5004). After addition of ABTS ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit IgG or Alexa Fluor 546 goat anti-rabbit IgG conjugates for 2 hours and mounted in VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Fluorescence images were taken using an inverted Nikon Eclipse TE2000-U microscope ...
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bioRxiv - Neuroscience 2023Quote: ... staining to visualize activated microglia was performed as previously described [48] using Iba1 primary antibody (Wako, 1:500) with Vectastain Rabbit Elite ABC-HRT Kit (Vector Labs, PK-6101). Second ...
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bioRxiv - Physiology 2023Quote: ... After blocking the non-specific binding with VECTASTAIN Elite ABC HRP Kit (Peroxidase, Anti-Mouse/Anti-Rabbit IgG) Kit (Vector Laboratories, CA, USA), the sections were incubated overnight with non-fluorophores primary antibody at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 0.1% Triton X-100 in TBS followed by 1 hr incubation with goat biotinylated anti-rabbit IgG secondary antibody (1:250, BA-1000, Vector Laboratories, RRID: AB_2313606) at room temperature and 30 min incubation with Vectastain Elite ABC reagent (PK-6100 ...
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bioRxiv - Microbiology 2023Quote: ... The wells were then incubated in a primary antibody blocking solution containing rabbit anti-GAP45 or guinea pig anti-CDPK1 or biotinylated dolichos (Vector labs B-1035) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Neuroscience 2023Quote: ... a blocking serum is added from the same species from which the secondary antibody is derived (ImmPRESS TM Anti-Rabbit and Anti-Mouse Kit from Vector Labs, Burlington, USA). The blocking was done at room temperature for a minimum of 20 minutes.
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-conjugated anti-goat or anti-rabbit secondary antibodies were each applied at 1:500 dilution and incubated with Vector elite Reagent (Vector Laboratories, CA, USA) for 45 minutes ...
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bioRxiv - Genetics 2024Quote: ... the slides were left at room temperature for 1 hour and then incubated for 30 min with the anti-mouse or anti-rabbit ImmPRESS HRP (peroxidase) secondary antibodies (Vector laboratories, CA, USA). Between all steps (except after the blockage of unspecific staining ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used for immunohistochemistry included biotinylated goat anti-rabbit (BA-1000) and biotinylated horse anti-goat (BA-9500) at a concentration of 1:200 (Vector laboratories, Burlingame, CA).
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used for chemiluminescence detection were HRP conjugated anti-mouse antibody (Cytiva, Cat# NA931) and anti-rabbit antibody (Vector Laboratories, Cat# PI10001). Near-infrared detection was carried out with IRDye® 680RD Donkey anti-Rabbit IgG (H + L ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were processed following ImmPRESS HRP Universal Antibody (horse anti-mouse/rabbit IgG) Polymer Detection Kit and ImmPACT DAB EqV Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA) protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Cell Biology 2024Quote: ... the slides were incubated with horse biotinylated anti-mouse/ rabbit IgG ABC-HRP peroxidase at room temperature for 1 hr using the VECTASTAIN Elite ABC Universal Kit (Vector Laboratories, PK-6200). The slides were then washed with 1X PBS for 10 min at room temperature and incubated with VECTASTATIN Elite ABC reagent (Vector Laboratories ...
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bioRxiv - Bioengineering 2024Quote: ... Then sections were incubated for 30 minutes at room temperature with anti-rabbit IgG peroxidase secondary antibodies (ImmPRESS reagent, Vector Laboratories, Burlingame, CA). Afterwards the sections were treated with hydrogen peroxide block (ab94710 ...
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bioRxiv - Bioengineering 2020Quote: ... Some sections were also immunostained with the antibody to neuron-specific nuclear protein NeuN (MAB377, 1:2K, Chemicon, Temecula, CA) and visualized using chromogen Vector Red (Vector Laboratories, Burlingame, CA). The stained sections were microphotographed using a 3-megapixel digital microscope camera (Spot RT ...
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bioRxiv - Zoology 2020Quote: ... followed by 10-min incubation with streptavidin-HRP (streptavidin conjugated to horseradish peroxidase in PBS containing stabilizing protein and anti-microbial agents, Vector Laboratories Inc., USA). Finally ...
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bioRxiv - Microbiology 2021Quote: ... After blocking of endogenous peroxidases sections were incubated with ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Laboratories, MP-7401-15) for 1h at RT and then with DAB substrate (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Sections were then washed for 2 minutes in PBS and incubated in the dark for 15 minutes with a secondary antibody solution containing Texas Red-conjugated anti-rabbit IgG (catalog #: TI-1000; Vector Laboratories, Burlingame, CA, USA), and Alexa Fluor 488-conjugated anti-mouse IgG (catalog # ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then washed in PBS and incubated for 1 hour in biotinylated anti-rabbit IgG (1:200; Vector Labs, Burlingame, CA, USA), rotating at RT ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibodies (goat anti-rabbit, Vector Laboratories inc., PI-1000, 1:1,000 and biotinylated horse anti-mouse, Vector Laboratories inc., PK-4002, 1:200) were added to the sections for 150 min ...
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bioRxiv - Pathology 2021Quote: ... we performed immunohistochemical staining using the avidin-biotinylated enzyme complex method and the VECTASTAIN Elite ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, CA, USA). The tissue sections were deparaffinized with xylene and ethanol ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...