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Citations for Vector Labs :
101 - 150 of 477 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 1h incubation of the following primary antibodies diluted in blocking buffer: biotinylated-dolichos biflorus agglutinin (DBA) [1:500] (Vector Laboratories), rabbit-anti SRS9 [1:1000] and mouse-anti SAG1 [1:1000](kindly provided by John Boothroyd ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNMT3A and DNMT3B was visualized in cross-section (5 µm) of paraffin-embedded tissues using specific primary antibodies and a Vectastain Elite ABC Kit (Vector Laboratories) or AlexaFluor 488-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... the appropriate secondary antibody-Alexa 488 conjugate (Molecular Laboratories) was applied at 1:500 dilution to detect the primary antibody and BS lectin at 1:1500 (Vector Laboratories) was applied along with the secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... Specificity controls for immunostaining included sections stained in the absence of primary antibody or in the presence of rabbit immunoglobulin G control antibody at 0.1 ug/mL (Vector Laboratories, Inc.). Sections were overlaid with Vectashield anti-fade mounting medium (Vector Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... A biotinylated goat anti-rabbit IgG was applied to detect rabbit primary antibodies and complexes were visualized using the R.T.U Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA). Mouse primary antibodies were visualized applying the VECTASTAIN® ABC HRP Kit (Peroxidase ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Detection of primary antibody was performed using the Super-sensitive–Polymer HRP system (Biogenex) and staining visualized using purple chromogen VIP (Vector Labs) and hematoxylin as a nuclear counterstain ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... using Dako Link48 plus as a service with the primary antibodies described in the Key Resources Table and species-appropriate ImmPRESS Polymer Detection Kit (Vector Laboratories) and developed with 3,3’-Diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... This procedure was modified for mouse primary antibodies for use with the Mouse on Mouse Immunodetection Kit (BMK-2202; Vector Laboratories) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS and blocked and stained using appropriate primary antibodies and reagents from the Vectastain Elite ABC kit (Vector Labs). Diaminobenzidine exposure was titrated to optimal contrast ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified 53BP1 Tudor domain was labeled at exposed primary amine groups with NHS-biotin using ChromaLINK NHS-Biotin protein labeling kit (Vector Laboratories). 1 equivalent of chromalink biotin was incubated with the 53BP1 Tudor domain for 2 h and buffer exchanged into fresh PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated with Alexa-Fluor-488-conjugated donkey anti-human IgG for 1.5 hours at room temperature and mounted with Vectashield containing DAPI (Vector Laboratories).
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bioRxiv - Neuroscience 2021Quote: ... re-blocked and probed with the relevant primary and secondary antibodies (bound to HRP) then developed with DAB + Nickel (SK4100, Vector Laboratories, UK). Chromogen development reactions were halted with distilled water ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Antibodies were as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently incubated with the respective primary and secondary antibodies (Table S3) using an ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) for 60 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... and permeabilization in 0.2% Triton (20 min on ice) by sequential incubation with primary and Texas red (TR)-labeled secondary antibodies (Vector Laboratories, Burlingame, Calif.) for 30 min each (all solutions in PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Anti-mouse monoclonal pS202/pT205 1:1000 (AT8 ...
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bioRxiv - Cell Biology 2022Quote: ... Engineered cartilage sheet sections were also treated with pronase and primary anti-Collagen Type II (DSHB II-II6B3) followed by VectaFluor R.T.U Antibody Kit DyLight® 488 (Vector Labs DI-2788) following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... was used as a secondary detection for primary antibodies raised in rabbit (CD4 and CD68) and Horse anti-goat IgG polymer reagent (Impress HRP)(Vector Laboratories 30036) for primary antibody MPO raised in goat ...
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bioRxiv - Microbiology 2023Quote: ... The wells were then incubated in a primary antibody blocking solution containing rabbit anti-GAP45 or biotinylated dolichos (Vector labs B-1035) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2019Quote: ... Incubation in the primary antibodies was followed by 2-hour incubation in anti-rabbit secondary biotinylated antibodies (1:200, Vector Laboratories, Burlingame, CA), and then in avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary visualization of the primary antibodies was completed with ImmPRESS Horse anti-rabbit Ig Kit (Catalog No. MP-7401-50, Vector Laboratories, Burlingame, CA) or ImmPRESS Horse anti-mouse Ig Kit (Catalog No ...
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bioRxiv - Microbiology 2023Quote: ... The wells were then incubated in a primary antibody blocking solution containing rabbit anti-GAP45 or guinea pig anti-CDPK1 or biotinylated dolichos (Vector labs B-1035) for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies with their respective concentrations were as follows Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (1:25, Vector Laboratories, #FL-1201), mouse monoclonal anti-3G5 IgM (1:10 ...
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bioRxiv - Cell Biology 2023Quote: ... tissue sections were incubated with the secondary biotinylated antibody corresponding to the animal species of the primary antibody (BA-1000; Vector Laboratories, Burlingame, CA) and exposed to 3,30-diaminobenzidine (SK-4105 ...
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bioRxiv - Neuroscience 2023Quote: ... staining to visualize activated microglia was performed as previously described [48] using Iba1 primary antibody (Wako, 1:500) with Vectastain Rabbit Elite ABC-HRT Kit (Vector Labs, PK-6101). Second ...
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bioRxiv - Cancer Biology 2023Quote: ... The blocking solution was blotted off and primary antibodies were diluted in 3% BSA with mouse-on-mouse kit (Vector Laboratories, #MKB-2213-1), and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS two times for 10 minutes and then primary antibodies were applied overnight in 10% normal horse serum (Vector Labs, #S-2000-20), 0.5% Triton-X-10 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a biotinylated anti-human IgG in TBST with 1% goat serum also incubated for 30 minutes at room temperature (Vector Labs, Burlingame, CA). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with primary antibody overnight at room temperature and detected using the appropriate biotinylated secondary antibody (1:200, Vector Labs, BA-1100, BA-2000) and alkaline phosphatase-Vector Red according to manufacturer instructions (Vector Labs) ...
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bioRxiv - Neuroscience 2023Quote: ... for the primary and Anti-glutamate receptor 1 (rabbit polyclonal) Goat Anti-rabbit HRP (Novus Biologicals Cat# NB7160, 1:250; Vector Laboratories, Burlingame, CA, USA) for the secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... primary antibody in Leica Primary Antibody Diluent for 30 min was followed by application of a rabbit anti-rat secondary (Vector Laboratories, Catalog Number AI-4001) for 8 min ...
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were mounted with Vectashield (Vector Laboratories) containing DAPI and imaged using an Olympus BX61 microscope equipped with DAPI and FITC-sensitive filters and a Hamamatsu ORCA-ER camera.
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bioRxiv - Cell Biology 2020Quote: ... Sperm cells were mounted (Vectashield, Vector Laboratories) and imaged with confocal microscopes (Zeiss LSM710 Elyra P1 and Olympus Fluoview 1000).
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bioRxiv - Cell Biology 2021Quote: ... Cells were mounted in Vectashield (Vector Laboratories) prior to application of a coverslip which was sealed using nail vanish ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were mounted in Vectashield (Vector Laboratories) containing 1ug/ml DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... cells were mounted with Vectashield (Vector Laboratories) with 4′,6-diamidino-2-phenylindole (DAPI).
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bioRxiv - Molecular Biology 2022Quote: ... cells were mounted in VECTASHIELD (Vector Laboratories).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were mounted in Vectashield (Vector Laboratories) and allowed to cure overnight ...
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bioRxiv - Microbiology 2022Quote: Cell membranes of A431D cells were labeled using 5μg/mL rhodamine labeled wheat germ agglutinin (Vector Labs; RL-1022) in HBSS (Hank’s balanced salt solution ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were counterstained with hematoxylin (Vector Labs, H3404) to detect total cells ...