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Citations for Vector Labs :
401 - 450 of 488 citations for Mouse FAM20B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... the slides were incubated with secondary antibody using an ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) for 60 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... incubation was for 2 hours at RT and secondary antibody (1:2000 in 1% BSA, Biotinylated horse anti-mouse, Vector Labs) incubation was for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with secondary antibodies (anti-rabbit, 1:400; DI-1594; anti-mouse, 1:1000; fl-2000; Vector laboratories, USA) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... The next day sections were washed twice with TBST and incubated with biotinylated anti-rabbit or anti-mouse antibodies (Vector Laboratories) diluted 1:200 in blocking buffer for 1h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... we used ImmPRESS duet staining HRP/AP polymer kits with anti-rabbit IgG-brown and anti-mouse IgG red (MP-7714, Vector Laboratories) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 1:1000) antibodies and biotin-conjugated anti-mouse secondary antibody (1:200) before applying the DAB peroxidase substrate kit (Vector laboratories) and imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a biotin-conjugated anti-rabbit or anti-mouse antibody (1:250, Vectastain Elite ABC kit, Vector laboratories). Sections were counterstained with Nissl (0.09% thionin ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by rinsing the sections in PBS and incubating them in a secondary antibody solution (biotinylated anti-rabbit for Arc and DARPP-32 and biotinylated anti-mouse for DCX and TH; 1:200; BA-1000, BA-2000 Vector Laboratories) for 2hrs ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a biotin-conjugated anti-mouse secondary antibody (1:200) and revealed with the DAB peroxidase substrate kit (Vector laboratories) before imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in primary antibody solution containing monoclonal mouse anti-PV (1:1000, #235, Swant) and FITC-conjugated Wisteria Floribunda Agglutinin (WFA, 1:500, FL-135, Vector Laboratories) overnight at 4 ℃ ...
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bioRxiv - Developmental Biology 2024Quote: ... 10% goat serum was added to all slides and incubated for 1 hour at RT followed by additional 1 hour mouse blocking (MKB 2213, Vector labs) for Wnt5a and LRP1 detection ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody (1:10,000 M.O.M. Biotinylated Anti Mouse Cat # MKB-2225-.1 or 1:1000 Goat Anti Rabbit IgG, Vector Laboratories Cat # BA-1000) was incubated for 2 h with 20% Superblock (Fisher Scientific Cat # 37535) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by a 10 min incubation with secondary antibody (either ImmPress-mouse HRP, MP-7402, or ImmPress-rabbit HRP, MP-7401, Vector Laboratories) and fluorescent labelling for 10 min using tyramide signal amplification with Opal dyes in the sequence of 690 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody double labelling was through an ImmPress Duet double staining anti-mouse HRP (brown)/anti-rabbit AP (magenta) kit (MP7724, Vector Laboratories, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with 3% NDS-PB-TX and first incubated in biotinylated secondary anti-mouse antibody (1:200, Vector Labs, CAMK-IIα amplification) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed 3 times for 5 minutes in 0.1 M PB and placed in goat anti-rabbit OR anti-mouse biotinylated secondary antibodies respectively (1:500, BP-9200/9100; Vector Labs, CA) for 6 hours ...
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bioRxiv - Biochemistry 2021Quote: ... or mouse anti-c-myc (hybridoma supernatant diluted 1:10, clone 9E10) or with biotinylated lectin Canavalia ensiformis agglutinin (ConA) (Vector Laboratories, France) in 1 μg/ml in TTBS (0.05% Tween-20/TBS pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were next incubated first with a biotinylated goat anti-rabbit IgG or horse anti-mouse IgG (BA-1000 and BA-2000 respectively, Vector Laboratories, USA), then with an avidin-biotin-complex conjugated to horseradish peroxidase (PK-6100 ...
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bioRxiv - Cancer Biology 2022Quote: ... and HRP-polymer-conjugated secondary antibody (anti-Rabbit, Goat and Rat from Vector Labs; anti-Mouse from Biocare. The slides were developed with Impact DAB (Vector Labs) and counterstained with hematoxylin ...
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bioRxiv - Immunology 2021Quote: ... the secondary biotinylated goat anti-mouse Ab was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Color was developed by incubating the slides with freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently incubated with the respective primary and secondary antibodies (Table S3) using an ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) for 60 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories, BA-1000, BA-9200, 1:500). The signal was developed using a standard Vectastain ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Following, the secondary antibody (The ImmPRESS Universal Antibody Kit, anti-mouse/rabbit) and ImmPACT DAB (both purchased from Vector Laboratories, Burlingame, CA) was applied according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBST before being incubated for two hours in 1:250 dilution of horse-anti-mouse (Vector Laboratories, UK), or ...
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bioRxiv - Neuroscience 2023Quote: ... non-specific binding was blocked for 30 minutes using 2.5% normal horse serum from the ImmPRESS Anti-Mouse IgG / Anti-rabbit IgG Reagent Kit (Vector Laboratories, CA, USA). (iii ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed with PBST three times and then incubated with biotinylated goat anti-mouse IgG antibody (Vector Laboratories, cat# BA-9200) for 2 hours RT ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary solution containing 1:2 ImmPRESS® (peroxidase) polymer horse anti-mouse IgG (rat absorbed) (Vector Laboratories) in TBS for 30 minutes ...
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bioRxiv - Pathology 2024Quote: ... The sections were washed three times with PBS before being placed in blocking solution with a fluorescein horse anti-mouse IgG (H+L) antibody and a Texas red goat anti-rabbit IgG (H+L) antibody (1:200; Vector Laboratories, Inc.). They were left there for two hours at room temperature to find VEGF and CD31 ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed and incubated with anti-BrdU antibody (1:100) followed by anti-mouse Ig-fluorescein antibody (1:200) and mounted onto a glass slide using DAPI (Vectashield, Vector Laboratories, CA, USA). Images were captured using fluorescence microscope BX53 (Olympus Corporation ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed 3 x 10 min in 0.1 M TBS and transferred to a secondary antibody solution with 1:200 anti-mouse IgG (Vector Laboratories, Burlington, ON, Canada) in TBS+ for 4 h at 4ºC ...
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bioRxiv - Neuroscience 2019Quote: ... using respective secondary antibodies (biotinylated donkey anti-rat IgG, donkey anti-mouse IgG, and donkey anti-rabbit IgG (1:500, Vector Laboratories, Burlingame, CA), and Alexa-488 donkey anti-rat IgG ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with Alexa Fluor conjugated anti-mouse and anti-rat antibodies (1:200 dilution) and mounted using the Vectashield without DAPI (Vector Laboratories, H-1000). Images were acquired as described above ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in a 1/500 dilution of biotinylated mouse-anti-rabbit (Vector Laboratories, Burlingame, California) secondary antibody in diluent containing 2% NGS ...
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bioRxiv - Microbiology 2021Quote: ... while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories, California, United States) at 1:50 with 1% BSA and thereafter incubated with Vectastain Elite ABC reagent (Vectastain Elite ABC Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Specimens were then washed three times for 5min each in PBS and incubated with secondary anti-mouse/rabbit IgG (Vector Laboratories, MP-7500) or fluorescent-conjugated secondary antibodies (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies listed in Table 2 were detected using ImmPress HRP Universal antibody polymer detection kit (Horse Anti-mouse/rabbit IgG, Vector Labs, MP-7500), as previously described for mouse in50 ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... Washes were then performed 3 x 10 min in TBS-0.1% Tween 20 (TBST) prior to secondary antibody incubation with ImmPRESS HRP horse anti-mouse IgG (Vector Labs, Cat# MP-7402), for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Immunohistochemical staining of tissue sections with no anti-mouse IgG antibody and the use of anti-Rabbit IgG antibody (Vector Laboratories, Burlingame, CA) were used for negative control analysis (data not shown) ...
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bioRxiv - Neuroscience 2019Quote: ... sections were transferred to a 1:250 dilution of biotinylated horse-anti-mouse IgG (for sections reacted against Crym; Vector Labs, Peterborough, UK) or biotinylated horse-anti-rabbit IgG (for sections reacted against Gng2 ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were then rinsed through another series of 1x PBS washes and incubated in either biotinylated anti-mouse IgG or anti-goat IgG secondary antibody [1:200] (Vector Laboratories, Burlingame, CA). The signal was amplified using a Vectastain ABC kit (Vector Laboratories ...
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bioRxiv - Pathology 2020Quote: ... slides were rinsed in PBS and incubated in a horse anti-mouse/rabbit biotinylated IgG secondary antibody (VECTASTAIN Elite ABC Kit, Vector Labs, PK-6200). Sections were rinsed again ...
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bioRxiv - Cell Biology 2021Quote: ... or immunostained with rabbit anti-mouse polyclonal Ror2 (provided by Dr. Yasuhiro Minami, Kobe University) with DAB visualization (Vector Laboratories, Cat #SK-4100), and counterstained with hematoxylin and eosin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... and sections were blocked with 1.5% normal serum (PK-6102, horse serum, mouse IgG or PK-6101, goat serum rabbit IgG; Vector Laboratories, Burlingame, CA, USA) for 1 h followed by incubation overnight at 4°C with anti-VEGF monoclonal antibody (SC-7269 ...
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bioRxiv - Neuroscience 2022Quote: ... Excess antibody buffer was removed with PBSTx and followed by incubation in biotinylated goat anti-mouse secondary antibody (1:1000 Cat# BP-9200-50: Vector Laboratories, California USA) overnight at 4°C in PBSTx and 1% NGS ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody incubation was with horse anti-mouse IgG conjugated to biotin (1:100 in PBS; Vector Labs, catalog BA-2000-1.5) for 2 h at room temperature ...