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Citations for Vector Labs :
101 - 136 of 136 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Antibody signal of Cytokeratin 8 was amplified using biotinylated anti-rat antibody (1:300, 0.5 mg, FisherScientific Vector Laboratories, CAS#NC9016344), followed by staining with Streptavidin ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... sections were incubated with peroxidase-conjugated secondary antibody (ImmPRESS goat anti-rat IgG; Vector laboratories, cat. no. MP-7444 ; 30min. RT). Finally ...
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bioRxiv - Neuroscience 2023Quote: 5µm thickness brain tissue sections from different groups of rats (n = 3 at each time point for each rat) were used for IHC using R.T.U Vectastain Universal Elite® ABC Kit (Vector Laboratories, USA) using standard protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were rinsed with Wash Solution before application of a blocking solution from an anti-rat ImmPRESS Detection kit (#MP7444-15, Vector Labs) for 20 min ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed for 3 x 5 minutes in 0.1 M PBS and mounted on glass slides using Vectashield medium (containing DAPI as required, Vector Labs, Peterborough, UK) and cover-slipped.
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3x in 0.1M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by boiling for 20 min in a 0.01 M sodium citrate (pH 6.0) solution and endogenous biotin was blocked using the Avidin/Biotin Blocking Kit (Vector Labs, SP-2001). Slides were incubated overnight with primary antibodies against anti-rabbit H3K4me3 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen retrieval was performed by microwaving the samples for 15 minutes in 0.01 M citrate buffer (pH = 6, Vector Laboratories, H-3300) in Millipore water ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed for 3 times in 0.1 M PBS and mounted on glass slides using Vectashield medium containing DAPI (Vector Labs, Peterborough, UK).
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bioRxiv - Neuroscience 2023Quote: ... samples were rinsed in 0.1 M tris-buffered saline (TBS) for 10 min prior to a 10 min incubation in BLOXALL (Vector Laboratories, SP-6000) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Neuroscience 2024Quote: Slices containing biocytin fills were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer overnight and processed with Vectastain ABC (avidin-biotinylated HRP complex) Elite Kit (Vector Labs, Burlingame, CA). The shape of the slice ...
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bioRxiv - Cancer Biology 2022Quote: ... and HRP-polymer-conjugated secondary antibody (anti-Rabbit, Goat and Rat from Vector Labs; anti-Mouse from Biocare. The slides were developed with Impact DAB (Vector Labs) and counterstained with hematoxylin ...
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bioRxiv - Developmental Biology 2019Quote: ... AF138, 1:1,000), anti-K8 (rat supernatant, Troma1), anti-K14 (Covance, AF64, 1:1,000) or UEA1 lectin (Vector Labs, X0922, 1:400). Secondary detection was performed with donkey anti-primary species ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary solution containing 1:2 ImmPRESS® (peroxidase) polymer horse anti-mouse IgG (rat absorbed) (Vector Laboratories) in TBS for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG, Vector Laboratories, PK-4004). Antibody binding was visualized with DAB-nickel (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... and goat anti-rat IgG (Jackson 112-035-143) at a dilution of 1:500 Cat: A110-109P along with ImmPACT DAB (Vector Labs, USA) to visualize the antigens ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed 3 x 10 min in 0.1 M TBS and transferred to a secondary antibody solution with 1:200 anti-mouse IgG (Vector Laboratories, Burlington, ON, Canada) in TBS+ for 4 h at 4ºC ...
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Corticocortical Connections of the Rostral Forelimb Area in Rats: A Quantitative Tract-Tracing StudybioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight in 0.1 M PBS with reagents “A” and “B” added according to Vectastain Elite Kit (Vector Laboratories, Burlingame, CA, #PK6100). Then ...
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bioRxiv - Molecular Biology 2020Quote: Five μm sections of fixed paraffin embedded kidney tissues (prepared as described for histopathology) were stained with Rhodamine labeled DBA (Vector Laboratory, # RL-1032) and Fluorescein labeled LTA (Vector Laboratory ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times in PBS and 2 times in 0.1 M phosphate buffer solution (10 min/wash) before being mounted and cover-slipped using Vectashield Vibrance w/DAPI (Vector Laboratories H-1800). Fluorescent conjugates of streptavidin were stored and prepared as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti rat (3052-08)) for 1 hour at room temperature and labelled structures were visualized using the ABC Vectastain kit (Vector laboratories, PK-6100). Finally ...
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bioRxiv - Neuroscience 2019Quote: ... using respective secondary antibodies (biotinylated donkey anti-rat IgG, donkey anti-mouse IgG, and donkey anti-rabbit IgG (1:500, Vector Laboratories, Burlingame, CA), and Alexa-488 donkey anti-rat IgG ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with Alexa Fluor conjugated anti-mouse and anti-rat antibodies (1:200 dilution) and mounted using the Vectashield without DAPI (Vector Laboratories, H-1000). Images were acquired as described above ...
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bioRxiv - Bioengineering 2020Quote: ... After the primary antibody, then treated with biotinlated donkey anti-rat IgG (LifeTech Thermo, A18743) followed by DAB staining using ABC kit (Vector Labs PK-6100) and DAB (Sigma D5637) ...
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bioRxiv - Cancer Biology 2020Quote: ... All rat primary antibodies were incubated for either 20 or 30 minutes followed by biotinylated rabbit anti-rat linker antibody (BA-4001, Vector Labs, Burlingame, CA) applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800 ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... primary monoclonal rat anti mouse Mac1 antibodies (AbD Serotec, Raleigh, NC, USA) were used along with secondary biotinylated goat anti-rat antibodies (Vector Laboratories, Burlingame, CA, USA). Each midbrain section was viewed at low power (10x objective) ...
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Microbiology 2020Quote: ... The detection of antibody binding was performed with anti-rabbit IgG HRP-conjugated polymer (Zytomed GmbH, Berlin, Germany) or anti-rat IgG HRP-conjugated polymer ImmPRESS™ (Vector Laboratories, Burlingame, CA, USA) and 3-amino-9-ethylcarbazole (AEC ...
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bioRxiv - Immunology 2023Quote: ... primary antibody in Leica Primary Antibody Diluent for 30 min was followed by application of a rabbit anti-rat secondary (Vector Laboratories, Catalog Number AI-4001) for 8 min ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary rabbit anti-rat IgG and tertiary goat anti-rabbit IgG with horseradish peroxidase (HRP; ImmPRESS® HRP) was purchased from Vector Laboratories (Newark, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed with 0.1 M PB (4 × 5 min) and incubated in biotinylated goat anti-mouse IgG antibody (Vector Laboratories: 1/200 in 2% NGS in 0.1M PB) for 30 min ...