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Citations for Vector Labs :
501 - 550 of 2098 citations for 7 chloro 5 2 fluorophenyl 4 hydroxy 3H 1 4 benzodiazepin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were finally mounted with Vectashield Mounting Media with 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat# H-1200). Stainings were digitalized employing the Olympus BX61VS microscope and scanner software VS-ASW-S6 (Olympus ...
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bioRxiv - Genomics 2021Quote: ... Flies were dissected 10 to 40 days pi and organs viewed by fluorescence microscopy and imaged live or fixed in 2% paraformaldehyde and stained with DAPI in VECTASHIELD mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Developmental Biology 2021Quote: ... For IBA1 staining sections were subsequently incubated with a biotinylated F(ab’)2-Goat anti-Rabbit IgG (Vector Labs, CA, USA). All sections were then incubated with horseradish peroxidase (HRP)-conjugated streptavidin (Dako Agilent Pathology Solutions ...
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bioRxiv - Immunology 2019Quote: ... HER-2-positive cells were visualized using rabbit IgG VECTORSTAIN ABC Elite and DAB (3,3’-diaminobenzidine) Peroxidase Substrate Kits (Vector Laboratories, UK) and visualized using a bright field microscope.
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bioRxiv - Cell Biology 2020Quote: ... Muscle tissues were then placed into a microcentrifuge tube containing PBT with 2 % final concentration of normal goat serum (NGS; Vector Labs) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... two times for 2 min each before reacting with diaminobenzidine in the presence of nickel (DAB Peroxidase Substrate Kit, Vector Laboratories) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories).
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bioRxiv - Genomics 2022Quote: ... sections were incubated with Alexa dye–conjugated secondary antibodies diluted in blocking serum for 2 hours before being washed again and mounted with Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories). Alexa Fluor® 488 Donkey IgG ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed (three times in 1xPBS for 10 min) and blocked for 2 hours with 10% normal goat serum (NGS, Vector Laboratories), 0.2% BSA (Carl Roth ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dehydrated by incubating for 2 min in increasing concentrations of EtOH (70%, 95%, and 100%) before mounting with Vectashield with DAPI (Vector laboratories). Images of at least 150 nuclei were acquired at 20X using a Zeiss Imager.M2 epifluorescence microscope equipped with a Axiocam 503 mono camera ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then incubated for 2 hours at room temperature with horseradish-peroxidase-labelled secondary antibody (Goat Anti-Rabbit IgG Antibody; Vector Laboratories) diluted 1:10,000 in 5% dried non-fat milk powder in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed in 1x PBS three times for 2 minutes and covered with Vectashield mounting medium without DAPI (Vector Labs). Five imaging fields in the gray matter of the same case were captured using a fluorescent microscope (Keyence BZ-X710) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were washed three times with 1x PBS at RT before either staining with 3uM DAPI (4’,6-diamidino-2-phenylindole) in 1x PBS for 10 min or mounting in Vectashield fluorescent mounting medium with DAPI (Vector Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The cells were then postfixed in cold methanol and mounted using Vec-taShield containing 4ʹ,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Images were acquired by a DMI6000B microscope and a DFC360 FX monochrome camera (both Leica Mi-crosystems).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Cancer Biology 2024Quote: Coated gold slides were silanized by VECTABOND® Reagent (Vector Laboratories, SP-1800-7) per the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... blocked with 5% BSA/5% normal goat serum (Vector Laboratories) in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... then mounted on slides in hard set mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (Vectashield, H-1500, Vector Laboratories, Burlingame, CA). A confocal system (TCS SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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bioRxiv - Immunology 2023Quote: ... we used the lectin from Datura stramonium at a staining concentration of 10 µg/ mL (Biotinylated DSL, Vector laboratories, B-1185-2). 2 × 105 cells per condition were collected and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 30-80 MΩ) lowered vertically and filled with a solution of potassium acetate (0.5M) and Neurobiotin (2%, Vector Laboratories, Burlingame, CA, USA). The signal was amplified and low-pass filtered (10kHz ...
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Neuroscience 2022Quote: ... diluted 1:1000 in the presence of 5% normal horse serum (Vector Laboratories inc, Burlingame, CA), Next day ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The prepared slides were stained and mounted with 7 µl DAPI in Vectashield (Vector Lab) and metaphase I chromosomes visualised using a Nikon 90i Eclipse fluorescent microscope with NIS Elements software ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by washes in 2xSSC pH 7 and then mounted with Vectashield (Vector Laboratories, H1200). Images were acquired using a Zeiss Cell Observer.
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bioRxiv - Systems Biology 2021Quote: ... #415190-9041-000) were treated with UV light for 1 h and coated with APES (3-aminopropyltriethoxysilane) using Vectabond reagent (Vector Laboratories, Burlingame, CA, USA, cat. #SP-1800-7) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... TC neurons in 250 micron brain slices or RGCs in flat-mount retinas were loaded by passive diffusion with 2% neurobiotin (Vector Labs, Burlingame, CA) and/or 100 μM CF568 dye (Biotium ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies listed in Table 2 were detected using ImmPress HRP Universal antibody polymer detection kit (Horse Anti-mouse/rabbit IgG, Vector Labs, MP-7500), as previously described for mouse in50 ...
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bioRxiv - Neuroscience 2021Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Molecular Biology 2019Quote: ... DAB staining was developed under a light microscope for between 2-10 min as per the manufacturer’s instructions with the ImmPACT DAB peroxidase substrate kit (Vector Labs, Cat# SK-4105). Slides were then counterstained with Hematoxylin Solution ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 μl/ml avidin and 6 μl/ml biotin; 2 hours at room temperature; Vector Laboratories, CA, USA), washed in PB ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were mounted onto gelatinized slides and left to dry (at least 2 hours) before being coverslipped with VectaShield (Vector Laboratories, Burlingame, CA). The sections were imaged with 10x objective and 10x eyepiece magnification on a Leica LSI 3000 microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit IgG or Alexa Fluor 546 goat anti-rabbit IgG conjugates for 2 hours and mounted in VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Fluorescence images were taken using an inverted Nikon Eclipse TE2000-U microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 2 × 30s in 1M tris-HCl solution and then incubated in a DAB reaction solution (Vector Labs, SK-4100) for 5-15 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.4 containing 0.1% Tween 20) and squashed on a glass slide with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Newark, California, USA). Observations were performed using an Olympus BX61 microscope (N = 45-193 individuals per strain).
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bioRxiv - Neuroscience 2023Quote: The procedure was similar to that of single-cell recordings except that the micropipette was filled with 2% Neurobiotin (SP-1120, Vector Laboratories, CA., USA) in 0.5 M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...