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Citations for Vector Labs :
501 - 548 of 548 citations for 7 Bromo 4 hydrazino 2 propylquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... then mounted on slides in hard set mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (Vectashield, H-1500, Vector Laboratories, Burlingame, CA). A confocal system (TCS SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary solution containing 1:2 ImmPRESS® (peroxidase) polymer horse anti-mouse IgG (rat absorbed) (Vector Laboratories) in TBS for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... we used the lectin from Datura stramonium at a staining concentration of 10 µg/ mL (Biotinylated DSL, Vector laboratories, B-1185-2). 2 × 105 cells per condition were collected and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS and incubated in biotinylated goat anti-rabbit (Vector labs, 1:500 in PBS-TX for 2 hrs). Sections were washed in PBS and then incubated in avidin-conjugated peroxidase (Vector labs ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody labeling was amplified with biotinylated goat anti-rabbit IgG for 2 hr (1:500 in PBS, BA-1000, RRID: AB_2313606; Vector Laboratories; Burlingame, CA) followed by Vectastain ABC Solution for 1 hr (1:500 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Then a secondary biotinylated goat anti-rabbit antibody was applied in PBST / 2% NGS (1:200; Vectastain, Vector Laboratories, Burlingame, CA, USA) (75 min ...
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bioRxiv - Neuroscience 2023Quote: ... 30-80 MΩ) lowered vertically and filled with a solution of potassium acetate (0.5M) and Neurobiotin (2%, Vector Laboratories, Burlingame, CA, USA). The signal was amplified and low-pass filtered (10kHz ...
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... Incubation in the primary antibodies was followed by 2-hour incubation in anti-rabbit secondary biotinylated antibodies (1:200, Vector Laboratories, Burlingame, CA), and then in avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Neuroscience 2019Quote: ... TC neurons in 250 micron brain slices or RGCs in flat-mount retinas were loaded by passive diffusion with 2% neurobiotin (Vector Labs, Burlingame, CA) and/or 100 μM CF568 dye (Biotium ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in a 1/500 dilution of biotinylated mouse-anti-rabbit (Vector Laboratories, Burlingame, California) secondary antibody in diluent containing 2% NGS ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies listed in Table 2 were detected using ImmPress HRP Universal antibody polymer detection kit (Horse Anti-mouse/rabbit IgG, Vector Labs, MP-7500), as previously described for mouse in50 ...
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bioRxiv - Neuroscience 2021Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Molecular Biology 2019Quote: ... DAB staining was developed under a light microscope for between 2-10 min as per the manufacturer’s instructions with the ImmPACT DAB peroxidase substrate kit (Vector Labs, Cat# SK-4105). Slides were then counterstained with Hematoxylin Solution ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 μl/ml avidin and 6 μl/ml biotin; 2 hours at room temperature; Vector Laboratories, CA, USA), washed in PB ...
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bioRxiv - Microbiology 2020Quote: ... Cells were counterstained with VECTASHIELD® Antifade Mounting Medium containing 1.5 µg ml- 1 4,6-diamidino-2-phenylindole (Vector Laboratories, Burlingame, CA, USA). Images were captured on a BX61 microscope equipped with a DP80 camera (Olympus Australia ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were mounted onto gelatinized slides and left to dry (at least 2 hours) before being coverslipped with VectaShield (Vector Laboratories, Burlingame, CA). The sections were imaged with 10x objective and 10x eyepiece magnification on a Leica LSI 3000 microscope ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed with 0.1 M TBS (5 × 5) and the stain was allowed to develop for 2 minutes using an ImmPACT novaRED Substrate Kit (Vector Laboratories, SK-4805). Tissue sections were then washed for 5 min in tap water ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit IgG or Alexa Fluor 546 goat anti-rabbit IgG conjugates for 2 hours and mounted in VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Fluorescence images were taken using an inverted Nikon Eclipse TE2000-U microscope ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.4 containing 0.1% Tween 20) and squashed on a glass slide with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Newark, California, USA). Observations were performed using an Olympus BX61 microscope (N = 45-193 individuals per strain).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 2 × 30s in 1M tris-HCl solution and then incubated in a DAB reaction solution (Vector Labs, SK-4100) for 5-15 min ...
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bioRxiv - Neuroscience 2023Quote: The procedure was similar to that of single-cell recordings except that the micropipette was filled with 2% Neurobiotin (SP-1120, Vector Laboratories, CA., USA) in 0.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were then incubated over night at 4°C on rotating wheel (or 2 h at RT) with a secondary antibody coupled to a fluorescent dye (DyLight® 549 (VECTOR lab # DI 1549) (1:400 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Antigen retrieval was performed by heating slides to 93 ± 2 °C for 20 min in high-pH antigen unmasking solution (H-3301, Vector Labs, Burlingame, CA, USA), followed by blocking in 5% bovine serum albumin (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Bioengineering 2023Quote: ... Migrated cells located at the bottom side of the transwell membrane were permeabilized with 0.1% Triton X-100 in PBS and the transwell membrane was coverslipped with mounting medium containing DAPI (Vector Labs, Cat # H-1800-2). For each transwell ...
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bioRxiv - Immunology 2023Quote: ... The brain lysate was 1:10 diluted in the PBS and stained with rhodamine-labeled dolichos biflorus agglutinin (DBA-red, Vector Laboratories, RL-1032-2). GFP and DBA-red double-positive cysts were counted and diluted to 5 cysts per 200 uL of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells stained with DAPI followed by wash in 2x SSC buffer before being mounted using VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories H-1700-2).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was incubated at least 2 hr with 30 μl package volume of Wheat-germ (WGA) -conjugated agarose beads (Vector Laboratories, Inc., Burlingame, CA) on a rotating mixer at 4°C and washed 3 times with lysis buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... glasses with the labeled cells were placed onto the Superfrost Plus adhesion slides (Epredia, EPBRSF41296SP) in 20 µl VECTASHIELD Vibrance Antifade Mounting Media (Vector Laboratories, H-1700-2) and stored at +4℃ in the dark.
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bioRxiv - Microbiology 2023Quote: ... Parasites were then stained with an anti-VSG-2 monoclonal antibody (Hovel-Miner et al., 2012) conjugated to Alexa647 and mounted in Vectashield (Vector Laboratories, H-1800-10) containing 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Genetics 2024Quote: ... or Cy5 (1:1000; USBIO 518482) conjugated Lotus Tetragonolobus Lectin (LTA) and Rhodamine conjugated Dolichos Biflorus Agglutinin (DBA) (1:1000; Vector Laboratories RL-1032-2), was added to co-stain proximal tubules and collecting ducts ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 mL/mL avidin and 6 mL/mL biotin; 2 hr at room temperature; Vector Laboratories, CA, USA, #PK-6100), washed in PB ...
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bioRxiv - Cancer Biology 2021Quote: ... After a 5 min wash with PBS the slide was incubated for 30 min with the Vectastain Elite ABC reagent followed by a 2-10 min incubation with a DAB peroxidase substrate solution (Vector Laboratories, Cat. No. SK-400) according to the manufacturer’s instructions ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... The slides stained with rabbit anti-SARS-CoV-2 S polyclonal antibody or rabbit anti-ACE2 antibody were further incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, cat. no. BP 1100). All slides were then incubated with HRP-conjugated streptavidin (Vector Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplementary Table 2 and the following reagents: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50). Following the application of TSA fluorophores (listed in Supplementary Table 2 ...
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bioRxiv - Genetics 2022Quote: ... and either directly seeded on 10cm cell culture dishes or further enriched with Lotus Tetragonolobus Lectin (LTL; Vector labs biotynilated beads, cat. no. B-1325-2) LTL or Dolichos Biflorus Agglutinin (DBA ...