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Citations for Vector Labs :
151 - 200 of 799 citations for 6 tert Butyl 4 chlorothieno 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and then mounted with Vectashield® mounting media with 4’,6-diamidino-2-phenylindole (DAPI; H-1000 and H-1200; Vector laboratories, Inc, Burlingame, USA). Potential unspecific binding was evaluated by omission of the primary antibody in control sections that were otherwise processed similarly.
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were blocked for 2 to 3 hrs with 2 % heat inactivated goat serum (Vector Laboratories) and 4 mg/ml BSA (AppliChem ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The preparations were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI; 2 μg/ml) in Vectashield (Vector Laboratories, Peterborough, UK). Fluorescence signals were analysed using an Axioimager Z2 epifluorescence microscope (Zeiss ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 μl/ml avidin and 6 μl/ml biotin; 2 hours at room temperature; Vector Laboratories, CA, USA), washed in PB ...
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bioRxiv - Neuroscience 2022Quote: ... 3-diaminobenzidine plus nickel (DAB; Vector Laboratories Kit; 2-5 min reaction). Sections were analyzed with an Olympus BX51 upright microscope ...
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bioRxiv - Neuroscience 2022Quote: ... 3-diaminobenzidine plus nickel (DAB; Vector Laboratories Kit; 2-5 min reaction). Sections were washed in 0.1M cacodylate buffer and postfixed with 1% osmium and 1.5% potassium ferrocyanide in cacodylate buffer for 1 hr at RT after sections were dehydrated in an ascending gradient of ethanol (ETOH ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 mL/mL avidin and 6 mL/mL biotin; 2 hr at room temperature; Vector Laboratories, CA, USA, #PK-6100), washed in PB ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) was used (H 1200, Vector Laboratories, Burlingame, CA, USA, Lot # 2E0815). All tissue sections from experimental and control animals were stained at the same time and with the same antibody lot.
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bioRxiv - Neuroscience 2021Quote: ... Slides were mounted using Vectashield Antifade mounting medium with 4ʹ,6-diamidino-2-phenylindole (DAPI; Vector Laboratories). These studies were carried out with IRB approval from Houston Methodist Hospital (Pro00010377).
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bioRxiv - Biochemistry 2023Quote: ... postfixed in cold methanol and mounted using VectaShield containing 4ʹ,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Images were acquired by a DMI6000B microscope and a DFC360 FX monochrome camera (both Leica Microsystems).
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bioRxiv - Bioengineering 2024Quote: ... Nuclei were stained using VECTASHIELD mountant with 5’,6-diamidino-2-phenylindole nuclear stain (DAPI; Vector Laboratories). Samples were then mounted onto glass slides and visualised using an Axiophot microscope or Evos Cell imaging system (Thermo Fischer ...
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
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bioRxiv - Cell Biology 2022Quote: ... Intestine sections were rehydrated and antigens were unmasked for 30 min in a steamer with sodium citrate (10 mM, pH 6) (Ki67, GFP, p57, PCNA, cleaved caspase 3) or High pH (H-3301, Vector Laboratories) (Olfm4 ...
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bioRxiv - Genomics 2021Quote: ... Chromosomes were counterstained by 10 μg/ml 4Ͱ,6-diamidino-2-phenylindole (DAPI) in Vectashield antifade mounting medium (Vector Laboratories). Images were captured using an epifluorescence microscope BX61 (Olympus ...
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bioRxiv - Immunology 2023Quote: ... for 2 hours at 4°C and mounted with VECTASHIELD HardSet Antifade Mounting Medium (Vector Laboratories) followed by imaging with BZ-X800 microscope (Keyence) ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and markers used were biotinylated WFA (4 µg/ml, B-1355-2, Vector Laboratories), guinea pig polyclonal anti-NeuN antibody (1-2 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... before incubating overnight at room temperature in 0.1 M Tris-HCl (pH 9.5) containing nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate at the recommended concentrations (Vector Laboratories). After washing in PBS and postfixing in 4% PFA ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 3-4 washes with PBST and mounting in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200). Images from two biologically independent experiments were captured at the same time using Zeiss Axio Widefield (20x/0.8 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were washed three times with 1x PBS at RT before either staining with 3uM DAPI (4’,6-diamidino-2-phenylindole) in 1x PBS for 10 min or mounting in Vectashield fluorescent mounting medium with DAPI (Vector Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The cells were then postfixed in cold methanol and mounted using Vec-taShield containing 4ʹ,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Images were acquired by a DMI6000B microscope and a DFC360 FX monochrome camera (both Leica Mi-crosystems).
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bioRxiv - Developmental Biology 2021Quote: ... Slides were subsequently counterstained for 3 min with 2 μg/ml DAPI containing Vectashield mounting solution (Vector Laboratories) and sealed with nail varnish ...
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bioRxiv - Neuroscience 2021Quote: ... 2 % NiCl2 and 4 % DAB in Tris-buffer using a DAB Substrate Kit (Vector Laboratories, Burlingame, USA). NiCl2 enhances sensitivity and intensity of DAB precipitation product ...
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bioRxiv - Neuroscience 2021Quote: ... in PB for 2 days at 4°C (ABC reagent, VectaStain Elite ABC-HRP kit, Vector laboratories). After the 2 day incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Neuroscience 2023Quote: ... Neurobiotin (6 mM, Vector Labs) was added to the internal pipette solution to locate and reconstruct the recorded neurons in subdivisions of BNST after experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Microbiology 2020Quote: ... 4,6-diamidino-2-phenylindole (DAPI) was added to the embedding medium (4:1 Citifluor AF1 (Citifluor) and Vectashield (Vector Laboratories)) ...
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bioRxiv - Genetics 2020Quote: ... Avidin D blocking reagent (Vector Laboratories) was applied to the slide for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and Rhodamine Avidin D (Vector laboratories) for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were developed using ImmPACT-3 3’-diaminobenzidine (DAB; Vector Labs), sequentially dehydrated as previously described (Stoyka et al. ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Citrate-buffer at pH 6 (Vector Laboratories) antigen retrieval method was performed on samples required for collagen 1 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody overnight at 4 °C and revealed with a biotinylated goat anti-rabbit IgG secondary antibody (1:1,000, 2 hat RT, Vector Laboratories, VECTBA1000), the VECTASTAIN ABC Elite HRP kit (Vector Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Slices were then washed overnight at 4 °C in a biotinylated Wisteria Floribunda Agglutinin (WFA, 1:2000, Vector Laboratories, B-1355-2) followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Immunology 2020Quote: ... 3-diaminobenzidine (DAB) (Vector Laboratories). Mouse spleen ...
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bioRxiv - Pathology 2020Quote: ... 3’-diaminobenzidine (DAB; Vector Laboratories) was used as a substrate according to the manufacturer’s instructions and hematoxylin was used as a counter-stain ...
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bioRxiv - Cancer Biology 2023Quote: ... treated with biotinylated anti-avidin D (Vector Laboratories) for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with biotinylated anti-avidin D (Vector Laboratories) for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoreactive signals were developed by incubating sections in 3-3’-diaminobenzadine (DAB) reagent (Vector Laboratories). Sections were mounted on glass slides which were serially dehydrated in ethanol ...
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bioRxiv - Genomics 2023Quote: ... 4x posthybridization washes in 50% formamide in 2xSSC and immunodetection with fluorescein avidin D and biotinylated antiavidin D system (Vector Laboratories) were carried out ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...