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Citations for Vector Labs :
351 - 400 of 1824 citations for 6 Bromo 2 4 fluoro phenyl imidazo 1 2 a pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... containing 0.3% Tween-20 and blocked with PBS with 0.3% Triton-X100 and 2% normal hose serum (Vector Laboratories # S-2000 ...
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bioRxiv - Neuroscience 2020Quote: The next day sections were wash 6 × 10 min with wash buffer (1 × TBS, 0.05% triton X-100) and incubated with biotinylated antibody (Vector Laboratories) diluted 1:200 in secondary blocking buffer (1 × TBS pH 7.6 ...
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bioRxiv - Genetics 2021Quote: ... To reduce fluorescence fading most slides were mounted in Vectashield antifade mounting medium containing 4.6-diamidino-2-phenylindole (DAPI; Vector Laboratories). Slides stained with Alexa Fluor 660 were stained with DAPI and then mounted in the ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... blocked for 2 hours with a blocking buffer composed of 1xPBS, 0.1% Triton-X100, 2% BSA (A9418, SigmaAldrich) and 5% goat serum (S-1000, Vector Laboratories) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... They were then washed and incubated with biotinylated secondary antibody for 2 h at room temperature and developed with Elite ABC kit and 3,3-diaminobenzidine (Vector laboratories). Sections were mounted ...
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bioRxiv - Immunology 2019Quote: ... Slides were rinsed for 2 minutes and endogenous biotin was blocked using an Avidin/Biotin Blocking kit (Vector Laboratories, Inc.), according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... the sections were washed 3 times in PBS for 5 minutes each and mounted with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories) and sealed with nail polish ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite, PK-6000; Vector Laboratories) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody was detected using a biotinylated secondary antibody (Table 2) by avidin-biotin peroxidase method (ABC Kit, Vector Laboratories) using diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then exposed to diaminobenzidine for 2 minutes using the DAB Peroxidase HRP Substrate Kit (Vector Laboratories, SK-4100) then washed with 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in secondary antibody in 5% NGS for at least 2 overnights and mounted using Vectashield (Vector Laboratories H-1000). After each step ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 in blocking solution at 4 °C overnight followed by incubation with peroxidase-linked goat anti-rabbit IgG (1:10000, # PI-1000-1, Vector Laboratories). Chemiluminescent substrate (# 34095 ...
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bioRxiv - Neuroscience 2022Quote: ... After loading the micropipette with 4% Neurobiotin (Vector Laboratories, Burlingame, UK, dissolved in 1 M KCl), it was filled with a 1 M KCl solution ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4°C and stained with fluorescein-conjugated streptavidin (Vector Laboratories, Peterborough, UK, 1:500). Cells were counterstained with ProLong® Gold Antifade Reagent with DAPI for nuclei staining (Cell Signalling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 4 hours of staining with rhodamine conjugated PNA (1:250, Vector Labs RL 1072) and WGA alexa fluor 488 (1:250 ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were finally mounted with Vectashield Mounting Media with 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat# H-1200). Stainings were digitalized employing the Olympus BX61VS microscope and scanner software VS-ASW-S6 (Olympus ...
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bioRxiv - Genomics 2021Quote: ... Flies were dissected 10 to 40 days pi and organs viewed by fluorescence microscopy and imaged live or fixed in 2% paraformaldehyde and stained with DAPI in VECTASHIELD mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Developmental Biology 2021Quote: ... For IBA1 staining sections were subsequently incubated with a biotinylated F(ab’)2-Goat anti-Rabbit IgG (Vector Labs, CA, USA). All sections were then incubated with horseradish peroxidase (HRP)-conjugated streptavidin (Dako Agilent Pathology Solutions ...
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bioRxiv - Immunology 2019Quote: ... HER-2-positive cells were visualized using rabbit IgG VECTORSTAIN ABC Elite and DAB (3,3’-diaminobenzidine) Peroxidase Substrate Kits (Vector Laboratories, UK) and visualized using a bright field microscope.
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bioRxiv - Cell Biology 2020Quote: ... Muscle tissues were then placed into a microcentrifuge tube containing PBT with 2 % final concentration of normal goat serum (NGS; Vector Labs) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... two times for 2 min each before reacting with diaminobenzidine in the presence of nickel (DAB Peroxidase Substrate Kit, Vector Laboratories) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories).
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bioRxiv - Genomics 2022Quote: ... sections were incubated with Alexa dye–conjugated secondary antibodies diluted in blocking serum for 2 hours before being washed again and mounted with Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories). Alexa Fluor® 488 Donkey IgG ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed (three times in 1xPBS for 10 min) and blocked for 2 hours with 10% normal goat serum (NGS, Vector Laboratories), 0.2% BSA (Carl Roth ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dehydrated by incubating for 2 min in increasing concentrations of EtOH (70%, 95%, and 100%) before mounting with Vectashield with DAPI (Vector laboratories). Images of at least 150 nuclei were acquired at 20X using a Zeiss Imager.M2 epifluorescence microscope equipped with a Axiocam 503 mono camera ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then incubated for 2 hours at room temperature with horseradish-peroxidase-labelled secondary antibody (Goat Anti-Rabbit IgG Antibody; Vector Laboratories) diluted 1:10,000 in 5% dried non-fat milk powder in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed in 1x PBS three times for 2 minutes and covered with Vectashield mounting medium without DAPI (Vector Labs). Five imaging fields in the gray matter of the same case were captured using a fluorescent microscope (Keyence BZ-X710) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... pH 6), followed by an incubation with rabbit anti-Iba1 (WAKO, Japan; 1:2500) and subsequently a biotinylated secondary antibody (Vector laboratories, USA). Visualization ensued after using the ABC complex solution (Vector laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were blocked for 1 h in 0.3% PBS-Triton-X-100 with 6% normal goat serum (NGS; Vector Labs, S-1000), followed by incubation for 72 h at 4 ºC with anti-cFos rabbit primary antibody (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... then mounted on slides in hard set mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (Vectashield, H-1500, Vector Laboratories, Burlingame, CA). A confocal system (TCS SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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bioRxiv - Immunology 2023Quote: ... we used the lectin from Datura stramonium at a staining concentration of 10 µg/ mL (Biotinylated DSL, Vector laboratories, B-1185-2). 2 × 105 cells per condition were collected and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 30-80 MΩ) lowered vertically and filled with a solution of potassium acetate (0.5M) and Neurobiotin (2%, Vector Laboratories, Burlingame, CA, USA). The signal was amplified and low-pass filtered (10kHz ...
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were mounted on micro-scope slides using 4:1 Citifluor:Vectashield solution (VWR and Vector Laboratories) and stored at −20 °C ...
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bioRxiv - Neuroscience 2024Quote: ... before a 4-hour incubation in 1:500 biotinylated Goat anti-Rabbit IgG (H + L) (Vector Labs) secondary in 2.5% NGS in 1x TBS-0.3% Triton at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... TC neurons in 250 micron brain slices or RGCs in flat-mount retinas were loaded by passive diffusion with 2% neurobiotin (Vector Labs, Burlingame, CA) and/or 100 μM CF568 dye (Biotium ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies listed in Table 2 were detected using ImmPress HRP Universal antibody polymer detection kit (Horse Anti-mouse/rabbit IgG, Vector Labs, MP-7500), as previously described for mouse in50 ...
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bioRxiv - Neuroscience 2021Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Molecular Biology 2019Quote: ... DAB staining was developed under a light microscope for between 2-10 min as per the manufacturer’s instructions with the ImmPACT DAB peroxidase substrate kit (Vector Labs, Cat# SK-4105). Slides were then counterstained with Hematoxylin Solution ...