Labshake search
Citations for Vector Labs :
501 - 550 of 566 citations for 6 BROMO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... ovaries were fixed in 4% PFA and then mounted in Vectashield® mounting media with DAPI counterstain (Vector Laboratories, Burlingame, CA). Ovaries were then imaged using Differential Interference Contrast on a Zeiss Inverted Observer Z1 at 100X magnification.
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
-
bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
-
bioRxiv - Cell Biology 2019Quote: ... non-transfected organisms encysting for zero to 12 hr were incubated for 2 hr at 4°C with 2.5 μg GST-CBM49 conjugated to Alexa Fluor 594 and 12.5 μg of WGA (Vector Laboratories, Burlingame, CA) conjugated to Alexa Fluor 488 in 100 μl HBSS ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...
-
bioRxiv - Neuroscience 2022Quote: ... primary antibody overnight at 4 °C and revealed with a biotinylated goat anti-rabbit IgG secondary antibody (1:1,000, 2 hat RT, Vector Laboratories, VECTBA1000), the VECTASTAIN ABC Elite HRP kit (Vector Laboratories ...
-
bioRxiv - Immunology 2023Quote: ... This was followed by incubation in with biotinylated goat anti-rabbit and donkey anti-guineapig antibodies (Table S5) for 4 h at room temperature then in avidin–biotinylated horseradish peroxidase complex (Elite ABC; 1:300; Vector Laboratories) diluted in PBS for 3 h at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2% Tween-20 in PHEM buffer and mounted in Vectashield (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
-
bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
-
bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 in blocking solution at 4 °C overnight followed by incubation with peroxidase-linked goat anti-rabbit IgG (1:10000, # PI-1000-1, Vector Laboratories). Chemiluminescent substrate (# 34095 ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated first with a Biotinylated Goat Anti-Rabbit IgG Antibody (over-night at 4°C-Vector Laboratories), and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The polyps were kept for at least o/n at 4°C in a Vectashield mounting medium with DAPI (Vector Laboratories) before being mounted and imaged on a Leica SP8 confocal system ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were labeled with DAPI (1:5000 in PBSTx) overnight at 4°C and mounted with Vectasheild (CAT H-1000-10; Vector Laboratories).
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 × 5 min in PBS followed by blocking in 20% normal goat serum (NGS; Vector Labs S-1000, Burlingame, CA, USA) in PBS for 1 hr at RT ...
-
Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... then washed 3 times in TBS before applying secondary antibody for 30 minutes at room temperature (Cat# MP-7451, Vector Laboratories, Burlingame, CA, USA). Slides were washed twice for 5 minutes each before applying DAB for 2 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 × 5 min in TBS buffer and then incubated for 100 min in a solution of streptavidin Dylight 649 (Vector laboratories, SA5649, 1:100) in TBS buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
-
bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... Following one (cell lines) or 3 days expression (primary hippocampal cultures) cells were fixed using paraformaldehyde and coverslips mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA) with or without DAPI (cell lines) ...
-
bioRxiv - Physiology 2022Quote: ... cells were washed 3 times with PBS on ice and bound biotinyl tyramide was visualized with fluorescent streptavidin (Vector Laboratories, Inc., Burlingame, CA, USA) at 1:1000 in PBS for 30 minutes at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... The blocking solution was blotted off and primary antibodies were diluted in 3% BSA with mouse-on-mouse kit (Vector Laboratories, #MKB-2213-1), and incubated at 4°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... beads were stored at 4°C in PBS to reduce background before mounting in VECTASHIELD antifade mounting media (Vector Laboratories H-1000). Images were acquired on a confocal laser scanning microscope (Leica SP5) ...
-
bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C in blocking solution and biotinylated anti-rabbit IgG secondary antibody (1:3000, Vector Laboratories BA-1000) at RT for 1h ...
-
bioRxiv - Biophysics 2021Quote: ... The cells were subsequently fixed in 4% paraformaldehyde for 15 minutes and the coverslips were mounted with Vectashield + DAPI (Vector Laboratories, USA) on a glass slide ...
-
bioRxiv - Neuroscience 2022Quote: ... Delipidated sections in PBS were blocked with BSA 4 % and incubated with a 50 ug/ml solution of lectin conjugated with fluorescein (Vector Laboratories, FL1171), 24 h at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed in 0.01 PBS and incubated overnight at 4 °C with Alexa biotinylated goat anti-rabbit 488 (1:2000, Vector Laboratories, Burlingame, CA). After rinses ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2 % Tween-20 in PHEM buffer and mounted using Vectashield mounting media (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Slices were then washed overnight at 4 °C in a biotinylated Wisteria Floribunda Agglutinin (WFA, 1:2000, Vector Laboratories, B-1355-2) followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200 ...
-
bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
-
bioRxiv - Cell Biology 2019Quote: ... fixed in 4% paraformaldehyde 20 minutes (5 min in methanol for anti-GluRIIA) and subsequently blocked in 5% Normal goat serum (Vector laboratories #S-1000) in PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
-
bioRxiv - Bioengineering 2020Quote: ... gels were fixed with 4% paraformaldehyde and stained with 10 mg/mL rhodamine-labeled Griffonia simplicifolia lectin I (GS-1lectin; Vector Laboratories; Burlingame, CA). Five randomly selected fields were imaged per gel to an area of 3×3 mm and a depth of ∼500 µm using a Zeiss LSM 700 confocal microscope ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
-
bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
-
bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with primary antibodies overnight at 4°C and with secondary antibodies for 1 hr at room temperature (RT) before ABC-HRP (Vector Laboratories #PK-7100) treatment and DAB (Vector Laboratories #sk-4105 ...
-
bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
-
bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...