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Citations for Vector Labs :
551 - 600 of 1927 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.4 containing 0.1% Tween 20) and squashed on a glass slide with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Newark, California, USA). Observations were performed using an Olympus BX61 microscope (N = 45-193 individuals per strain).
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed again with PBS and blocked with 2.5% Normal Goat Serum for 20 minutes at room temperature (ImmPRESS Reagent, Vector Lab, cat#: MP-7451) and then incubated with the anti-rabbit ImmPRESS IgG peroxidase reagent (Vector Lab ...
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bioRxiv - Pathology 2024Quote: ... or anti-mouse (1:2,500, PI-2000-1, Vector Labs) secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2) ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC was performed on 4-μm sections with IHC was performed using Avidin/Biotin Blocking Kit (Vector Laboratories, SP-2001), Avidin-Biotin Complex kit (Vector Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... with VECTASHIELD® mounting medium containing 0.5 μg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Vector Laboratories, H-1200). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Cancer Biology 2022Quote: ... the sections were incubated with blocking buffer (PBS, 0.25% Triton-X-100, 10% goat serum, 4 drops/ml streptavidin (Vector Laboratories)) ...
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bioRxiv - Developmental Biology 2022Quote: ... nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in VECTASHIELD Mounting Medium (Vector Laboratories Inc., Burlingame, CA, USA) after washing ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed and stained for 30 min at 4°C with 5 μg/ml FITC- WGA (Vector Laboratories). After the wash ...
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bioRxiv - Immunology 2021Quote: ... Slides were counterstained and mounted with VECTASHIELD HardSet Antifade Mounting Medium with DAPI (4’,6-diamidino-2-phenylindole, Vector Laboratories). Staining was evaluated by confocal microscopy (TCS SP8 X ...
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bioRxiv - Developmental Biology 2019Quote: ... The brains were squashed between a slide and a coverslip before staining with 4′, 6-diamidino-2-phenylindole (DAPI, 0.5 μg/mL) and were mounted in Vectashield mounting medium (Vector Laboratories). To determine the frequency of metaphasic cells that show chromosomal aberrations (CABs ...
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bioRxiv - Developmental Biology 2019Quote: ... Ovaries were stained with 4′, 6-diamidino-2-phenylindole (DAPI, 0.5 μg/ml) and were mounted in Vectashield mounting medium (Vector Laboratories). Slides were analyzed under a Leica DM6000B wide-field fluorescence microscope equipped with a Hamamatsu ORCA-ER CCD camera and a HC PL FLUOTAR 20x/0.50NA objective ...
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bioRxiv - Molecular Biology 2019Quote: ... Slides were stained with 4′, 6-diamidino-2-phenylindole (DAPI, 0.5 µg/ml) and were mounted in Vectashield mounting medium (Vector Laboratories).
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bioRxiv - Biochemistry 2021Quote: ... The slides were washed 2X and mounted using 20µl of Vectashield mounting media containing 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI) (Vector Laboratories). Chromosome surface spreads were immunostained with rat –-HA at 1:200 (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) (500 nM) before mounting on slides using Vectashield (Vector Laboratories) to reduce photobleaching.
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bioRxiv - Neuroscience 2023Quote: ... The sections were then mounted with vector medium containing 4’,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (Vector Laboratories, USA). All images were examined on an Olympus confocal microscope (Olympus ...
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bioRxiv - Biophysics 2023Quote: ... The co-culture was incubated overnight at 4 °C with 10 mg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... Larvae were then washed twice in 1xSSC and 0.1xSSC 10Mm DDT before mounting with VECTASHIELD antifade mounting medium containing DAPI (4’,6-diamindio-2-phenylinole) (Vector laboratories). L4 were visualised using brightfield microscopy for length measurements ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with a mounting medium containing 4’,6-diamidino-2-phenylindole dihydrochloride solution (DAPI) (H-1200, Vector Laboratories).
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bioRxiv - Evolutionary Biology 2023Quote: ... Then slides were PBS and immersed into Vectashield with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA, USA). Slides were analyzed using a fluorescence light microscope Axio Imager D1 (Carl Zeiss ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were incubated with primary antibody overnight at 4°C then exposed to biotinylated anti-rabbit secondary antibody (Vector Labs, BA-1000 ...
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bioRxiv - Genomics 2023Quote: The aldehyde groups resulting in a 40 μl mix were then biotinylated using a mixture containing 4 μl of NaOAc (1M, pH 6.0) and 4 μl of Biotin (Long Arm) Hydrazide (100 mM, catalog num. SP-1100, Vector Laboratories). The 48 μl resulting mixture was next incubated for 30 minutes at 40°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was completed for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The dried slides were mounted with Vectashield (containing 4, 6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, P/N H-1200). Images were acquired with a DFC360 FX monochrome camera (Leica Microsystems ...
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bioRxiv - Biophysics 2024Quote: ... The co-culture was incubated overnight at 4 °C with 10 µg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Physiology 2023Quote: ... Samples were then washed three times for 15 minutes and mounted in Vectashield containing 1.5 µg/ml 4’,6-diamidino-2-phenylindole (DAPI; Vector Laboratories) and imaged using a Leica SP8 confocal.
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were then washed four times in 0.2 % PBST for 15 min each and stored at 4 °C in VectaShield (Vector Laboratories).
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bioRxiv - Molecular Biology 2023Quote: ... The slides were mounted with VECTASHIELD Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, H-1800) and were imaged using a Zeiss AxioScan Z.1 slide scanner (Carl Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in 3 x 5 min in TBST and mounted with Vectashield® mounting medium (Cat. No. H-1000; Vector Laboratories, USA).
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for two hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... As a last step the slides were washed in 1x PBS-T twice for 15 min at room temperature and mounted by adding 10-20 µl Vectashield mounting medium containing DAPI (Vector laboratories, #H-1200-10). The pictures were taken with a Leica TCS SP5 confocal microscope (pinhole 60.05 µm ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were blocked for 20 minutes with two drops of Vector Biotin Block (Avidin/Biotin Blocking kit. SP-2001. Vector Laboratories, Inc. Burlingame, CA). Samples were washed twice with PBS with Protein Block Serum-Free Ready-To-Use for 30 minutes at R.T ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were immerged in 3% H2O2 for 5 minutes to block endogenous peroxidase activity and non-specific binding was blocked with diluted normal horse serum for 20 minutes (Vectastain Universal Elite ABC kit, Vector Laboratories, Burlingame, California, USA). Sections were incubated 1 hour at room temperature with the following primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... Antigen retrieval was performed by heating slides to 93 ± 2 °C for 20 min in high-pH antigen unmasking solution (H-3301, Vector Labs, Burlingame, CA, USA), followed by blocking in 5% bovine serum albumin (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated in Tris-buffered saline/0.1% Tween-20 (TBS-T) containing 2.5% goat serum (included in ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Kit, Vector Laboratories, MP-MP-7451-15) to block non-specific binding ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS two times for 10 minutes and then primary antibodies were applied overnight in 10% normal horse serum (Vector Labs, #S-2000-20), 0.5% Triton-X-10 (Sigma ...