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Citations for Vector Labs :
401 - 450 of 535 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation of the sections in biotinylated anti-rabbit/mouse secondary antibody (1:1,000 dilution, Vector Laboratories, Burlingame, CA) and then in avidin-biotin-peroxidase complex (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was washed by PBS-TX (0.3% Triton X-100) and then incubated with 1:1000 dilution of biotinylated anti-mouse antibody (Vector Labs) with agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# 111-035-144] and Peroxidase-conjugated horse anti-mouse IgG (H+L) antibody [(1:5,000) (Vector laboratories, PI-2000)].
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bioRxiv - Neuroscience 2024Quote: ... before washing in PBS supplemented with 0.25 % Triton X and incubation in secondary antibody (ImmPRESS Reagent Anti-Mouse Ig, or Anti-Rabbit Ig, Vector Laboratories) for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Alexa Fluor donkey anti-goat) or conjugated to horseradish peroxidase (anti-rabbit and anti-mouse IgG [H+L], 1:8.000; Vector Laboratories).
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Co-release of GABA and ACh from medial olivocochlear neurons fine tunes cochlear efferent inhibitionbioRxiv - Physiology 2024Quote: ... a second blocking for ∼2 h at room temperature with a Mouse blocker Reagent (2-3 drops in 2.5 ml PBS, MKB-2213-1, Vector Laboratories) was used ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibody conjugates used for western blotting were HRP-linked horse anti-mouse IgG (Vector Laboratories, PI-2000, 1:10,000), goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... Prior to incubation with anti-rabbit or anti-mouse secondary HRP-conjugated antibodies (Vector Laboratories, PI□1000 and PI□2000), and afterwards membranes were washed for 30 min in PBS□T ...
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bioRxiv - Biochemistry 2020Quote: ... equivalent amounts of solubilized proteins from the supernatant (input) were added to 75 μl of WGA-bound agarose beads (Vector Laboratories) and incubated o/n at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... for 90 seconds in a low power microwave and 16 minutes in a steamer followed by protein blocking with 10% normal goat serum (S-1000, Vector Lab) and 1% bovine serum albumin (BP1600-100 ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV cross-reactive N protein 1C7C7 MAb (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified 53BP1 Tudor domain was labeled at exposed primary amine groups with NHS-biotin using ChromaLINK NHS-Biotin protein labeling kit (Vector Laboratories). 1 equivalent of chromalink biotin was incubated with the 53BP1 Tudor domain for 2 h and buffer exchanged into fresh PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 400-500 ±g of protein was then added to 50 µl of Wheat Germ Agglutinin (WGA) agarose bound beads (Vector Laboratories) filled to a volume of 300 µl with Lectin Binding Buffer (20 mM Tris pH 8 ...
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bioRxiv - Pathology 2023Quote: ... The cleared fractions of skeletal muscle samples and total protein extracts of the brain were incubated with 500 μl of succinylated wheat germ agglutinin (WGA)–agarose beads (Vector Labs) at 4°C for 16 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... for 15 min at room temperature and immunostained using the N protein 1C7C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Genetics 2021Quote: ... slides were washed 3 times in PBS-T for 5 min each and incubated with the biotinylated horse anti-mouse IgG antibody (AK-5002, Vector laboratories) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... or Alexa 647 donkey anti-mouse (1:200, Thermo A31571) and then mounted with the VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratory).
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the samples were washed with 1× TBS followed by incubation for 1 h at room temperature with a biotinylated secondary antibody (PK-6102, horse serum, mouse IgG or PK-6101, goat serum rabbit IgG; 1:3000 dilution; Vector Laboratories) in blocking buffer ...
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bioRxiv - Bioengineering 2020Quote: ... % secondary antibody (biotinylated horse anti-mouse IgG antibody – Col I and Col II; biotinylated goat anti-rabbit IgG antibody - ALP, Vector Laboratories) diluted in 2 (v/v ...
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bioRxiv - Genetics 2020Quote: ... Sections were incubated with primary antibody (supplementary table S4) overnight at 4°C and with universal biotinylated horse anti-mouse/rabbit immunoglobulin G secondary antibody (Vector Laboratories) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated for 5 hours at room temperature in VectaFluor DyLight 594 anti-mouse secondary antibody (3 drops in 5mL of 0.1% BSA, DK-8818; Vector Labs, CA). When incubation was completed ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200, Vector Laboratories, Burlingame, CA, USA), and then incubated for 1h in an avidin-biotin peroxidase complex (Vectastain ABC Elite PK6100 ...
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bioRxiv - Neuroscience 2020Quote: ... Corresponding secondary antibodies (1:500 goat anti-rabbit/goat anti-mouse biotinylated secondary antibody (Vector Laboratories, BA-1000 and BA-9200) were added ...
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bioRxiv - Neuroscience 2020Quote: ... 30 min in biotinylated goat anti-mouse or anti-rabbit IgG (1:200, PA-920 or BA-1000; Vector Laboratories, USA); 30 min avidin-biotin complex solution (PK-7100 ...
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bioRxiv - Microbiology 2021Quote: ... Infected cells were visualized by labeling with anti-flavivirus E-glycoprotein antibody using a mouse VECTASTAIN ABC-horseradish peroxidase (HRP) kit (PK-4002; Vector Laboratories) and a DAB peroxidase substrate kit (SK-4100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were incubated with antibodies against CD45 or MHC Class I for 30 mins at RT (Supplementary Table 7) and stained with biotinylated anti-mouse secondary antibody (Vector Lab) before counterstaining with haematoxylin ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then washed thrice in phosphate buffered saline prior to 30 min incubation with biotin-conjugated Goat Anti-Mouse IgG (H+L) (1:500, BP-9200, Vector Laboratories). Endogenous peroxidase was blocked and detection was performed using VECTASTAIN® ABC-HRP Kit with signal development carried out using 3,3-diaminobenzidine ...
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bioRxiv - Immunology 2020Quote: ... tissues were washed twice for 5 minutes in wash buffer and stained using the ImmPRESS universal (Anti-Mouse/Anti-Rabbit) kit (Vector labs).
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bioRxiv - Genetics 2020Quote: ... goat anti-rabbit IgG conjugated with either DyLight 488 or DyLight 549 and horse anti-mouse IgG conjugated with either DyLight 488 or DyLight 549 (Vector Laboratories). The lectin PNA labeled with fluorescein or rhodamine was obtained from Vector Laboratories.
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bioRxiv - Cancer Biology 2021Quote: ... the slides were incubated with secondary antibody using an ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) for 60 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The next day sections were washed twice with TBST and incubated with biotinylated anti-rabbit or anti-mouse antibodies (Vector Laboratories) diluted 1:200 in blocking buffer for 1h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... we used ImmPRESS duet staining HRP/AP polymer kits with anti-rabbit IgG-brown and anti-mouse IgG red (MP-7714, Vector Laboratories) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by rinsing the sections in PBS and incubating them in a secondary antibody solution (biotinylated anti-rabbit for Arc and DARPP-32 and biotinylated anti-mouse for DCX and TH; 1:200; BA-1000, BA-2000 Vector Laboratories) for 2hrs ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with secondary antibodies (anti-rabbit, 1:400; DI-1594; anti-mouse, 1:1000; fl-2000; Vector laboratories, USA) for 2 h ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by a 10 min incubation with secondary antibody (either ImmPress-mouse HRP, MP-7402, or ImmPress-rabbit HRP, MP-7401, Vector Laboratories) and fluorescent labelling for 10 min using tyramide signal amplification with Opal dyes in the sequence of 690 ...
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bioRxiv - Developmental Biology 2024Quote: ... 10% goat serum was added to all slides and incubated for 1 hour at RT followed by additional 1 hour mouse blocking (MKB 2213, Vector labs) for Wnt5a and LRP1 detection ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a biotin-conjugated anti-mouse secondary antibody (1:200) and revealed with the DAB peroxidase substrate kit (Vector laboratories) before imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in primary antibody solution containing monoclonal mouse anti-PV (1:1000, #235, Swant) and FITC-conjugated Wisteria Floribunda Agglutinin (WFA, 1:500, FL-135, Vector Laboratories) overnight at 4 ℃ ...
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bioRxiv - Bioengineering 2022Quote: ... incubation was for 2 hours at RT and secondary antibody (1:2000 in 1% BSA, Biotinylated horse anti-mouse, Vector Labs) incubation was for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 1:1000) antibodies and biotin-conjugated anti-mouse secondary antibody (1:200) before applying the DAB peroxidase substrate kit (Vector laboratories) and imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Genetics 2024Quote: ... goat anti-mouse for GFAP staining (1:1000) and rabbit anti-rat for CD68 staining (1:1000) (all from Vector laboratories). A 3,3’-diaminobenzidine reporter was used.
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bioRxiv - Microbiology 2024Quote: ... Triton X-100 in PBS for 15 min at RT and immunostained using an influenza virus anti-NP mouse monoclonal antibody (MAb) HT103 (1:100) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ImmPRESS® HRP Horse Anti-rabbit (MP-77452) or the Goat Anti-mouse (MP-7401) IgG Polymer kits from Vector Laboratories was used for all IHC staining ...
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bioRxiv - Neuroscience 2024Quote: ... made in horse, 1:250; anti-mouse made in horse, 1:250; anti-rabbit made in horse, 1:250, Vector Laboratories). Then ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed in PBS 1X three times for 10 min each and then incubated with biotinylated goat anti-mouse IgG or goat anti-rabbit IgG and visualized using an ABC reagent kit (Vector Laboratories) according to the manufacturer’s recommendations ...