Labshake search
Citations for Vector Labs :
351 - 396 of 396 citations for Vitamin D Depleted Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... free-floating sections were washed with PBS 3 times and blocked in 5% normal goat serum (NGS, Vector Laboratories, S-1000-20) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were washed with 1X Phosphate Buffered Saline-Tween (PBS-T) and treated with BLOXALL endogenous blocking solution for 10 minutes and blocked with 2.5% Normal horse serum (Vector Labs, Burlingame, CA) for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by blocking of nonspecific binding sites using normal goat serum (10%) and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA). Sections were incubated with phospho-SMAD2/3 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: ... The sections were washed with 0.1 m PBS three times for 15 min each and blocked for 1 hour with a solution of 10% normal goat serum (Vector Laboratories, USA) and 0.3% Triton X-100 (T8787 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a biotinylated anti-human IgG in TBST with 1% goat serum also incubated for 30 minutes at room temperature (Vector Labs, Burlingame, CA). Then ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Immunology 2019Quote: ... They were treated with 30% H2O2 in TBS for 30 minutes at room temperatures and then blocked with 15% normal goat serum (Vector Laboratories, Peterborough, U.K.) in 0.1% TBS-T (0.1% of Triton X-100 in 1× TBS ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 4% paraformaldehyde 20 minutes (5 min in methanol for anti-GluRIIA) and subsequently blocked in 5% Normal goat serum (Vector laboratories #S-1000) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... consisting of PBS containing 1% (w/v) Triton X-100 (A1388, AppliChem) and 10% (v/v) normal goat serum (S-1000, Vector Labs, Burlingame, CA) for 1 h at RT ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated at room temperature with 3% (v/v) H2O2 for 10 min to quench endogenous peroxidase activity and blocked by 5% (v/v) normal goat serum (Vector Laboratories, #S-1000) in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS (5 × 5 min) and incubated in biotinylated anti-rabbit secondary antibody (1:1000 in PBS with 0.1% bovine serum albumin, Vector Laboratories, Inc., Burlingame, CA) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with 3% H2O2 for 10 min and then blocked with 0.1% Triton X-100 in TBS containing 2% BSA and 2% normal goat serum (NGS, Vector Laboratories, Burlingame, CA, USA) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Endogenous peroxidases were quenched and endogenous mouse IgG in blood serum was blocked using the Mouse-on-Mouse blocking reagent (Vector Laboratories, Burlingame, CA). Subsequently ...
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bioRxiv - Neuroscience 2019Quote: ... sections were reacted for 1 hour in an avidin/biotin-peroxidase complex solution with 1% normal serum (ABC Elite, Vector Laboratories, Peterborough, UK). Sections were then washed twice in 0.05M Tris buffer and the label was visualized with 3,3-diaminobenzidine kit (DAB Substrate Kit ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS-TX (0.5% Triton X-100 in PBS) 6 x 20 min then blocked in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA, USA) for 1 h before being incubated on a shaker overnight with primary antibodies (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Neuroscience 2022Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Molecular Biology 2023Quote: ... non-specific binding sites were blocked with Tris-phosphate buffered saline (TPBS) supplemented with 10% normal goat serum (Vector Labs, Newark, CA, USA) and 3% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... fresh frozen brain slices on glass coverslips were incubated in PBS solution containing 10% heat inactivated goat serum (Vector Laboratories, Ref S-100) and 0.3% Triton X-100 for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed again with PBS and blocked with 2.5% Normal Goat Serum for 20 minutes at room temperature (ImmPRESS Reagent, Vector Lab, cat#: MP-7451) and then incubated with the anti-rabbit ImmPRESS IgG peroxidase reagent (Vector Lab ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 × 5 min in PBS followed by blocking in 20% normal goat serum (NGS; Vector Labs S-1000, Burlingame, CA, USA) in PBS for 1 hr at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for two hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were immerged in 3% H2O2 for 5 minutes to block endogenous peroxidase activity and non-specific binding was blocked with diluted normal horse serum for 20 minutes (Vectastain Universal Elite ABC kit, Vector Laboratories, Burlingame, California, USA). Sections were incubated 1 hour at room temperature with the following primary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... or goat anti-mouse IgG Alexa Fluor 647 (Thermo, A32728) were diluted 1:200 in blocking buffer and 1% normal goat serum (Vector Laboratories, S-1000-20) and incubated for 2 hours at room temperature in a dark container ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Neuroscience 2023Quote: ... We incubated free floating sections of the brain in H2O2 (1:50) for 30 minutes then blocked it with 10% Normal Horse Serum (Vector Laboratories, Burlingame, CA, USA) for one hour ...
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bioRxiv - Genetics 2023Quote: ... slides were blocked for one hour in 10% goat serum and incubated for one hour at room temperature using a M.O.M kit (Vector Labs Cat# BMK-2202; Newark, CA).
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated in Tris-buffered saline/0.1% Tween-20 (TBS-T) containing 2.5% goat serum (included in ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Kit, Vector Laboratories, MP-MP-7451-15) to block non-specific binding ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed in PBS two times for 10 minutes and then primary antibodies were applied overnight in 10% normal horse serum (Vector Labs, #S-2000-20), 0.5% Triton-X-10 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... hBO sections were washed once with 1x PBS and blocked in blocking buffer containing 10% normal goat serum (NGS, Vector labs: S-1000-20), 0.1% BSA and 0.3% Triton-X in 1× PBS for one hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... a biotinylated form of the α-D-galactose specific isolectin I-B4 from Griffonia simplicifolia (GSB4; 1:500; B-1205; Vector Laboratories, Burlingame, CA, USA; 1:500) for activated microglia detection ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1 h at RT and then stained with EC specific biotinylated isolectin B4 (Vector lab, B-1205-.5, 1:100 dilution) overnight at 4 °C and followed by staining with FITC- streptavidin (Invitrogen ...