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Citations for Vector Labs :
301 - 350 of 357 citations for Tris 4 pyridin 4 ylethynyl phenyl amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each slide was coverslipped using a mounting medium that contained the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA) and stored at −20°C until image acquisition occurred.
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Neuroscience 2024Quote: ... Cover slips were added and sealed with Vectashield mounting medium plus 4’,6-diamindino-2-phenylindole (DAPI) to visualise nuclei (Vector Laboratories, Burlingame CA, USA). Slides were imaged with an upright confocal microscope (Zeiss LSM880)
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bioRxiv - Evolutionary Biology 2024Quote: ... Chromosomes and nuclei were stained with 2 ng/µl DAPI (4’,6-diamidino-2-2phenylindole) in Vectashield antifade medium (Vector Laboratories, Burlingame, CA, USA). Preparations were analysed with an Axiolmager M2 epifluorescent microscope (Carl Zeiss ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on a glass microscope slide using a 4:1 mixture of Citiflour™ AFI mountant solution to Vectashield® vibrance antifade mounting medium (Vector Laboratories, USA). The fluorescence images were taken using an Echo Revolve R4K (Upright & Inverted Capability ...
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bioRxiv - Neuroscience 2024Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were incubated with the primary antibody overnight at 4 °C and processed using the VECTASTAIN® Elite ABC-HRP Rabbit IgG Kit (Vector Laboratories, #PK-6101) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight with agitation at 4°C with Wisteria floribunda lectin (WFA, WFL, Biotinylated; Vector Laboratories, Newark, CA, B-1355-2) diluted in staining buffer (2% PBST and 2% NGS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryosections were blocked in PBS containing 10% donkey serum and 0.1% Triton X-100 for 2 h and incubated overnight at 4°C with rabbit anti-Iba-1 and goat anti-Arg1 antibodies or FITC-conjugated Lycopersicon esculentum (Tomato) Lectin (Vector Labs, FL-1171-1). Next ...
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bioRxiv - Cell Biology 2023Quote: ... antigen retrieval with either citrate-or Tris-based solution (Vector Laboratories), and blocking with 5% goat serum ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Developmental Biology 2021Quote: ... and then mounted with Vectashield® mounting media with 4’,6-diamidino-2-phenylindole (DAPI; H-1000 and H-1200; Vector laboratories, Inc, Burlingame, USA). Potential unspecific binding was evaluated by omission of the primary antibody in control sections that were otherwise processed similarly.
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Area X (n = 4) and (ii) HVC (n = 3; [14]. The sections were rinsed in PBS, subjected to antigen retrieval (Vector Laboratories, H-3300, pH 6) and treated with 2N HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in slow agitation with primary antibody and/or biotinylated Wisteria Floribunda lectin (WFA, 1:1000, Vector Labs, USA, Ref. B-1355-2). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... heated up in Tris-based buffer (Vector Laboratories, Cat# H-3301-250) in the pressure cooker to accomplish antigen unmasking ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed with 0.1 M PB (4 x 5 min) and incubated in biotinylated goat anti-mouse IgG antibody (Vector Laboratories: 1/200 in 2% NGS in 0.1M PB) for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed with high pH Tris-based Antigen unmasking solution (Vector Labs), then washed (H2O ...
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bioRxiv - Immunology 2020Quote: ... and underwent antigen retrieval by microwaving in Tris-based antigen unmasking solution (Vector Labs). REAL peroxidase Blocking agent (Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by Tris base (pH 9.0) dependent protein antigen retrieval (Vector Labs, Burlingame, CA) at 95°C ...
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bioRxiv - Pathology 2023Quote: ... rehydrated and antigen unmasking was realized with pH 9 Tris solution (H-3301, Vector Laboratories) 5 min three times in a microwave ...
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bioRxiv - Cell Biology 2021Quote: Tissue Slides were subjected to antigen retrieval with Tris buffer PH=8.0 (Vector Labs H-3301) in a pressure cooker ...
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bioRxiv - Cancer Biology 2024Quote: ... before undergoing antigen retrieval using citrate or tris based antigen unmasking solution (Vector laboratories, H3300, H3301). The slides were then blocked with 8% BSA (Fisher Bioreagents ...
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bioRxiv - Microbiology 2021Quote: ... Antigen unmasking was performed using Tris-based antigen unmasking solution pH 9.0 (Vector Laboratories ref # H-3301) by boiling the slides in the unmasking solution for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... then for 5 min at 10% power in Tris-based antigen unmasking solution (Vector Laboratories H-3301) in a standard microwave oven ...
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bioRxiv - Microbiology 2022Quote: ... Antigen unmasking was performed using Tris-based antigen unmasking solution pH 9.0 (Vector Laboratories ref # H-3301) by boiling the slides in the unmasking solution for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... Antigen unmasking was performed using Tris-based antigen unmasking solution pH 9.0 (Vector Laboratories ref # H-3301) by boiling the slides in the unmasking solution for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... tissue slides were incubated overnight at 60°C in Tris-based antigen unmasking solution (Vector Laboratories, Burlingame, CA) before permeabilization in 3% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval of FFPE tissue sections was performed with Tris buffer at pH 8.0 (Vector Labs H-3301) in a pressure cooker for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... before probing with appropriate affinity-purified rabbit hCAP-H2 antibody in 1% BSA in TBS (25 mM Tris [pH 7.5] and 0.15M NaCl) followed by anti-rabbit horseradish peroxidase (Vector Laboratories) in TBS-T ...
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bioRxiv - Immunology 2021Quote: ... Tissues were then subjected to antigen retrieval in either Tris- or citric acid-based antigen unmasking solutions (Vector Laboratories), blocked in 10% normal goat serum ...
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bioRxiv - Cancer Biology 2023Quote: ... and antigenic sites were unmasked using either Tris-EDTA (10mM Tris-HCl, pH 9.0; 1mM EDTA; 0.05% Tween-20) or citric acid-based unmasking solution (Vector Laboratories) in Pascal pressure chamber (Dako) ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed by heating the paraffin-embedded tissue sections in Tris-EDTA buffer (Vector Laboratories, CA, USA). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... Antigen unmasking was performed using Tris-based antigen unmasking solution (pH 9.0) by boiling the slides in the unmasking solution for 20 min (Vector Laboratories). Quenching of endogenous peroxidase was performed using 0.3% hydrogen peroxide solution (Abcam Cat # ab64218) ...
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bioRxiv - Developmental Biology 2021Quote: ... heat-induced epitope retrieval was performed by immersing slides in Tris-EDTA buffer (Cellgro) pH 9.0 or citrate buffer (Vector Laboratories) pH 6.0 in a 95°C water bath for 35min ...
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bioRxiv - Developmental Biology 2022Quote: ... In case antigen retrieval was needed slides were heated to 90°C in a Tris-based buffer (Vector Laboratories, H-3301) The slides were allowed to cool for at least 30 min and washed in 1xPBST before incubation with primary antibodies The respective primary antibodies were diluted in 1% NGS overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were washed with Tris buffered saline and then incubated with biotinylated anti-rat IgG (10 μg/ml, BA-4001, Vector laboratories) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... antigen retrieval was performed by boiling slides in citrate-based or Tris-based antigen unmasking buffer (Vector Labs H3300 and H3301) for 45 minutes ...
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bioRxiv - Physiology 2024Quote: ... slides were rinsed 3 times in Tris-Buffered Saline (TBS) and then incubated in BLOXALL Endogenous Enzyme Blocking Solution (cat# SP-6000, Vector Laboratories) for 15 min in dark to quench endogenous peroxidase ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 min of running water before antigen retrieval in Tris EDTA buffer pH 9.0 (Vector Laboratories H-3301-250) or Citrate-based pH 6.0 (Vector Laboratories H-330-250 ...
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bioRxiv - Immunology 2020Quote: ... and nonspecific epitopes (species-specific serum diluted 1:5 in Tris-buffered saline and 5% bovine serum albumin, or blocking serum from species specific ImmPRESS® kit; Vector Laboratories) and incubated with primary antibody (Table 2 ...