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Citations for Vector Labs :
301 - 350 of 354 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... PB and E samples were subjected to SDS-PAGE and Western blotting followed by biotin detection using the Vectastain ABC AmP Reagent and Duolux chemiluminescent substrate (Vector Labs) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2019Quote: ... then incubated with ImmPress HRP Polymer Detection Reagents (depending on species of primary; rabbit, MP-7401; mouse, MP-6402-15; goat, MP-7405; all Vector Laboratories) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The ImmPRESS Polymer Reagent was incubated at room temperature for 30 minutes and the signal was developed with ImmPact DAB (SK-4105, Vector Laboratories). The tissue was then rinsed with tap water for 5min to stop the reaction ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were cut to a thickness of 0.5 μm using a diamond knife on an Ultracut microtome (Reichart-Jung) and collected on multiwell slides (ICN Biomedicals) coated with Vectabond reagent (Vector Laboratories). Calcofluor staining and immunolocalisation with anti-HG antibodies JIM7 and LM20 was conducted as previously described (Lee et al. ...
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bioRxiv - Physiology 2022Quote: ... After reducing non-specific binding with 30 min of Animal-Free Blocker incubation followed by 1 h incubation with Mouse on Mouse Blocking Reagent (Vector Laboratories), sections were incubated with primary antibodies (Proinsulin ...
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bioRxiv - Cell Biology 2019Quote: ... The sections were rinsed in PBS before incubation for 30 minutes with diluted biotinylated “universal” secondary antibody and 30 minutes with the ready-to-use Vectastain Elite ABC Reagent (Vector Laboratories), followed by a 5 minutes-incubation with 3,3’-diaminobenzidine (DAB) ...
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bioRxiv - Genetics 2019Quote: ... washed in PBS and then incubated in 2.5% horse blocking serum (ImmPRESSpress anti-rabbit IgG reagent Kit, Vector Laboratories, Burlingame, CA) for 1 hour at room temperature ...
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bioRxiv - Genetics 2019Quote: ... incubated with the ImmPRESS™ Reagent for 30mins at room temperature before addition of ImmPACT DAB chromogen (SK-4105, Vector Laboratories). Sections were then counterstained with Hematoxylin ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody (ImmPRESS Reagent Kit, MP-7401, Vector Laboratories, Germany). AEC-Plus (K3469 ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissues were incubated in biotinylated secondary antibodies for 30 minutes at room temperature and treated with VECTASTAIN Elite ABC Reagent (Vector Laboratories) per the manufacturer’s instructions ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Coverslips (24×30 mm and 22×22 mm #1.5) were washed and treated with VECTABOND® Reagent (Vector Laboratories, Newark, CA) and then coated with 25% polyethylene glycol (PEG) ...
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bioRxiv - Neuroscience 2022Quote: ... the serum in the blocking and antibody steps was replaced with reagents from the Mouse-on-Mouse (M.O.M.) Basic Immunodetection Kit following vendor instructions (Vector Labs #BMK-2202). These antibodies were mouse anti-viperin/Cig5 ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS and blocked and stained using appropriate primary antibodies and reagents from the Vectastain Elite ABC kit (Vector Labs). Diaminobenzidine exposure was titrated to optimal contrast ...
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bioRxiv - Cell Biology 2023Quote: ... Sections and myofibers that were stained with anti-Pax7 antibody (DSHB) also included a block with 1:50 Mouse on Mouse Ig Blocking Reagent (MKB-2213, Vector Labs).
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bioRxiv - Cell Biology 2024Quote: ... nuclei were labeled with DAPI (as in section Reagents and treatments) and samples were mounted with Vectashield (H-1000-10, Vector Laboratories).
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bioRxiv - Developmental Biology 2020Quote: ... immunohistochemistry was carried out using heat-induced epitope retrieval buffers (A. Menarini) and Vectastain avidin-biotin-HRP reagents (Vector Lab PK-6100). Anti-BAP1 antibody (Cell signalling ...
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bioRxiv - Neuroscience 2019Quote: ... Triton-followed by 1 h incubation with 0.5% (v/v) Mouse on Mouse (M.O.M.™) Blocking Reagent (Vector Laboratories, #MKB-2213-1). Sections were then stained with primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody (ImmPRESS Reagent Kit, MP-7401, Vector Laboratories, Germany) with AEC+ (K346 ...
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bioRxiv - Cell Biology 2021Quote: ... before peroxidase staining was conducted for 30 min with a peroxidase staining kit (VECTASTAIN® ABC Reagent, cat # PK-4000, Vector Laboratories) as described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 1-hr in kit-specific blocking reagent (ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Vector Laboratories, MP-7451), and incubated for 24-hr at 4°C with primary antibody (Table S1) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 5 times again and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat. # H-1400). Thalamocortical slice imaging was performed on an Andor Dragonfly microscope described below.
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bioRxiv - Neuroscience 2023Quote: ... non-specific binding was blocked for 30 minutes using 2.5% normal horse serum from the ImmPRESS Anti-Mouse IgG / Anti-rabbit IgG Reagent Kit (Vector Laboratories, CA, USA). (iii ...
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bioRxiv - Cancer Biology 2023Quote: ... A cHL FFPE section was mounted on a No.1 glass coverslip pre-treated with VECTABOND Reagent (Vector laboratories, SP-1800-7) as described above ...
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bioRxiv - Neuroscience 2023Quote: ... and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG, Vector Laboratories, PK-4004). Antibody binding was visualized with DAB-nickel (VWR ...
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bioRxiv - Immunology 2023Quote: ... was used as a secondary detection for primary antibodies raised in rabbit (CD4 and CD68) and Horse anti-goat IgG polymer reagent (Impress HRP)(Vector Laboratories 30036) for primary antibody MPO raised in goat ...
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bioRxiv - Genomics 2021Quote: ... and afterwards with a secondary peroxidase-conjugated anti-rabbit/-mouse IgG antibody (MP-7401, ImmPress Reagent Kit for FLI1, p-MYBL2 and SOX6, PK6200, Vectastain Elite ABC HRP Kit for PAX7; Vector Laboratories, Burlingame, USA). Target detection was performed using DAB+chromogen (Agilent Technologies ...
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Corticocortical Connections of the Rostral Forelimb Area in Rats: A Quantitative Tract-Tracing StudybioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight in 0.1 M PBS with reagents “A” and “B” added according to Vectastain Elite Kit (Vector Laboratories, Burlingame, CA, #PK6100). Then ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Endogenous peroxidases were quenched and endogenous mouse IgG in blood serum was blocked using the Mouse-on-Mouse blocking reagent (Vector Laboratories, Burlingame, CA). Subsequently ...
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bioRxiv - Cancer Biology 2019Quote: ... Reactive sites were identified with biotinylated antimouse and anti-rabbit secondary antibodies and peroxidase ABC reagents (Vector-Elite, Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s instructions and peroxidase activity was visualized with DAB+ reagents (DakoCytomation) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were again rinsed five times in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed with PBS and incubated with the following blocking solutions for 1 hour at room temperature: Mouse Ig Blocking Reagent in Mouse on Mouse (MOM) kit from Vector Laboratories (PK-2200) for staining with antibodies against Cbl (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in a solution containing avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories; 1:50) for 2hrs ...
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The slides were then washed with 1X PBS for 10 min at room temperature and incubated with VECTASTATIN Elite ABC reagent (Vector Laboratories, PK-6200) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-conjugated anti-goat or anti-rabbit secondary antibodies were each applied at 1:500 dilution and incubated with Vector elite Reagent (Vector Laboratories, CA, USA) for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed again with PBS and blocked with 2.5% Normal Goat Serum for 20 minutes at room temperature (ImmPRESS Reagent, Vector Lab, cat#: MP-7451) and then incubated with the anti-rabbit ImmPRESS IgG peroxidase reagent (Vector Lab ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Bioengineering 2024Quote: ... Then sections were incubated for 30 minutes at room temperature with anti-rabbit IgG peroxidase secondary antibodies (ImmPRESS reagent, Vector Laboratories, Burlingame, CA). Afterwards the sections were treated with hydrogen peroxide block (ab94710 ...
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bioRxiv - Molecular Biology 2021Quote: ... the slides were incubated with a biotin-conjugated secondary antibody for 1 h and with VECTASTAIN ABC Reagent (Cat. #PK- 4000; Vector Laboratories, Burlingame, CA, USA) for 30 min at 20°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Neuroscience 2021Quote: ... 30 μm thick slices containing either the VTA or NAc core were collected and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat #H-1400) and imaged using an Olympus FV1000 confocal microscope (Olympus Scientific Solutions).
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplemental Table 1b and the following reagents: ImmPRESS(r) HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50), ImmPRESS(r ...
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bioRxiv - Microbiology 2021Quote: ... was used to stain nucleic acids following washing away unbound secondary antibodies and samples were covered with VectaShield anti-fade reagent (Vector Laboratories, VWR, Cat# 101098-048) and a coverslip ...
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bioRxiv - Cancer Biology 2021Quote: ... After a 5 min wash with PBS the slide was incubated for 30 min with the Vectastain Elite ABC reagent followed by a 2-10 min incubation with a DAB peroxidase substrate solution (Vector Laboratories, Cat. No. SK-400) according to the manufacturer’s instructions ...