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Citations for Vector Labs :
301 - 350 of 1052 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were washed twice in PBS (5 min) and mounted using Vectashield without DAPI (Vector Laboratories Inc., Burlingame, CA, USA) for immediate visualization by microscopy.
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 immunohistochemistry (IHC) was performed on 5 μm sections using a mouse on mouse immunodection kit (Vector Laboratories BMK-2202). Briefly ...
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bioRxiv - Pathology 2023Quote: ... The sections were washed with PBST (3x, 5 min) and developed using a DAB substrate kit (Vector Laboratories, Burlingame, CA, USA). Counterstaining was performed with hematoxylin ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the fixed specimens were washed thrice for 5 min in 1x phosphate buffered saline (1xPBS; pH 7.4) and mounted using Vectashield mounting medium (H-1000, Vector Laboratories, CA). FITC-albumin and 10 kD dextran specimens were imaged using a ZEISS LSM900 confocal microscope with ZEISS Zen acquisition software (blue edition ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 0.5 μg/mL DAPI in PBS for 5 min prior to drying with ethanol and were mounted using Vectashield mounting medium without DAPI (Vector Laboratories). Images were acquired and analysed using a Leica fluorescence microscope ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were washed three times in PBTx for 15 min followed by five 5-minute washes in PBS and mounted in Vectashield antifade mounting medium containing DAPI (Vector Laboratories). Samples were imaged on an Olympus FV3000 Confocal Laser Scanning Microscope and processed in Imaris.
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
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bioRxiv - Biochemistry 2022Quote: ... Fixative was washed out with a continuous flow of 50 μl blocking buffer (5% normal goat serum S1000 in low-salt CSFxB; Vector Labs), then reactions were incubated in that buffer for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Organs were washed three times in PBS for 5 min per wash and mounted in Vectashield (Cat# H-1000, Vector Laboratories).
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bioRxiv - Neuroscience 2024Quote: ... The sections were again washed three times 5 min and then mounted with Vectashiled HardSet Antifade Mounting Medium (#H-1400, Vector Laboratories) on gelatin-coated slides ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 min of running water before antigen retrieval in Tris EDTA buffer pH 9.0 (Vector Laboratories H-3301-250) or Citrate-based pH 6.0 (Vector Laboratories H-330-250 ...
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bioRxiv - Biochemistry 2024Quote: ... 250 μM CuSO4 and 2.5 mM sodium ascorbate were mixed as a reaction pre-mix with 100 μM of alkyne-label: TAMRA-alkyne (Vector Labs), biotin-PEG4-alkyne (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were shortly stained with 5 µg DAPI (Applichem A1001) and mounted on glass slides using Vectashield (Vector Labs H-1000). The distribution of Flag-eIF2A was analysed on a confocal microscope (Leika TCS SP8 ...
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bioRxiv - Neuroscience 2024Quote: ... samples were cleared in 70% glycerol in PBS for 5 min at room temperature and then mounted in Vectashield (Vector Labs). Confocal microscope images were taken under 10x or 63x magnification using a Zeiss LSM800.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed three times in PBST-PVA for 5 min and mounted in Vectashield with DAPI (H-1200-10, Vector Laboratories) under #1 coverslips supported by Dow Corning high-vacuum silicone grease (Z273554-1EA ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Cancer Biology 2024Quote: ... Sialic acid expression was determined using biotinylated SNA (α-2,6 sialic acid) and MALII (α-2,6 sialic acid) lectins purchased from Vector Laboratories. 1 × 105 6419c5 cells were incubated with 2 µg/mL of biotinylated lectin for 45 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained at room temperature with 5 μg/mL fluorescein-conjugated wheat-germ agglutinin (WGA, Vector Laboratories catalog no. FL-1021) for 15 minutes to detect exposed chitin or 50 μg/mL fluorescein-conjugated concanavalin A (ConA ...
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bioRxiv - Neuroscience 2021Quote: Free-floating parallel brain sections were washed with PBS + 0.5% Triton X-100 (PBS-T) and then blocked with 5% normal goat serum (NGS, #S1000, Vector laboratories, Burlingame, CA) in PBS-T for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Antibodies were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2020Quote: ... The peroxidase reaction was produced by incubating the sections for 5 to 12 minutes in a DAB substrate solution (SK-4100; Vector Labs, CA). Reacted tissue was then mounted on gelatin coated slides ...
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bioRxiv - Cell Biology 2020Quote: ... DAPI was applied as a nuclear counter stain at 0.3 μM for 5 minutes prior to mounting with medium (Vectashield soft, Vector Labs, Peterborough, UK) and placing the coverslips upside down onto microscope slides ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 5 times again and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat. # H-1400). Thalamocortical slice imaging was performed on an Andor Dragonfly microscope described below.
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed twice in PBS for 5 min and mounted on a drop of VECTASHIELD solution (Vector Laboratories, H-1200-10). Images were acquired using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Genetics 2024Quote: ... Cover slips were subsequently washed with Wash buffer B for 5 minutes and mounted onto the slides with Vectashield® Mounting Medium (H-1000, Vector Laboratories). Images were taken at the Imaging Facility ...
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bioRxiv - Microbiology 2024Quote: ... the specimens were washed in PBS for 5 min each and cover with glass slides using mounting medium (Vector Laboratories, United States). For imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Anti-mouse monoclonal pS202/pT205 1:1000 (AT8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were washed with 1x PBS for 5 min and antigen retrieval was performed with Antigen Unmasking Solution Citrate-based pH 6.0 (Vector Laboratories H-3300) in a Decloaking Chamber™ (Biocare Medical ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Immunohistochemistry on 5 µm sections from frozen or paralin-embedded tissues was performed using the biotin-peroxidase method (Vector laboratories, Burlingame, CA). IHC staining and scoring were evaluated by a trained pathologist.
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bioRxiv - Neuroscience 2024Quote: ... followed by washing in ultrapure water for 5 min and mounted with Vectashield Vibrance® Antifade Mounting Medium (# ZKO8O3, Vector Labs, US) with or without DAPI (0.001 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... The incubation with the primary antibody is done for 5 hours followed by biotinylated mouse secondary (Vector Labs, MOM Kit BMK-2202) in 5.75μg/mL ...
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bioRxiv - Physiology 2024Quote: ... Cardiac sections were then washed in PBST and labeled with FITC conjugated Wheat-Germ Agglutinin (1:500; Vector Labs #FL-1021-5) and Goat anti-Mouse IgM Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was rinsed again three times (5 minutes each) in 0.01M PBS before immersing in an avidin-biotin complex (ABC; Vector labs, PK-4000) in the same concentration PBS for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...