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Citations for Vector Labs :
301 - 350 of 840 citations for 4 2 CHLORO 3 PYRIDYL 4 OXOBUTYRONITRILE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... They were then blocked with 10% goat serum in PBS-Triton 0.1% (T-PBS) containing 4 drops/ml of Avidin solution (of the Avidin/Biotin Blocking Kit, Vector Labs SP-2001) for 1 hour and then incubated overnight with the primary antibody (Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... De-waxed and rehydrated sections were heated up and then pressure cooked for 4 minutes in antigen unmasking solution (H-3300, Vector Laboratories Inc). Once cooled ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were developed using ImmPACT-3 3’-diaminobenzidine (DAB; Vector Labs), sequentially dehydrated as previously described (Stoyka et al. ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fixed with 4% paraformaldehyde and stained with 10 mg/mL rhodamine-labeled Griffonia simplicifolia lectin I (GS-1lectin; Vector Laboratories; Burlingame, CA). Five randomly selected fields were imaged per gel to an area of 3×3 mm and a depth of ∼500 µm using a Zeiss LSM 700 confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with primary antibodies overnight at 4°C and with secondary antibodies for 1 hr at room temperature (RT) before ABC-HRP (Vector Laboratories #PK-7100) treatment and DAB (Vector Laboratories #sk-4105 ...
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bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were subsequently fixed in 4% PFA and stained for ALP using the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories SK-5300) and Von Kossa using a 4% silver nitrate solution and exposing cultures to 2400 kJ of ultraviolet light using a UV Stratalinker.
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: ... The proximal end of the femur was first dipped in phosphate-buffered saline with 0.5% Triton-X (PBST) for 2-3 seconds and immediately transferred to a Vaseline® well of neurobiotin 488 tracer (Vector Labs, USA) solution prepared in 1M NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on a glass microscope slide using a 4:1 mixture of Citiflour™ AFI mountant solution to Vectashield® vibrance antifade mounting medium (Vector Laboratories, USA). The fluorescence images were taken using an Echo Revolve R4K (Upright & Inverted Capability ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were incubated with the primary antibody overnight at 4 °C and processed using the VECTASTAIN® Elite ABC-HRP Rabbit IgG Kit (Vector Laboratories, #PK-6101) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryosections were blocked in PBS containing 10% donkey serum and 0.1% Triton X-100 for 2 h and incubated overnight at 4°C with rabbit anti-Iba-1 and goat anti-Arg1 antibodies or FITC-conjugated Lycopersicon esculentum (Tomato) Lectin (Vector Labs, FL-1171-1). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Immunology 2020Quote: ... 3-diaminobenzidine (DAB) (Vector Laboratories). Mouse spleen ...
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bioRxiv - Pathology 2020Quote: ... 3’-diaminobenzidine (DAB; Vector Laboratories) was used as a substrate according to the manufacturer’s instructions and hematoxylin was used as a counter-stain ...
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoreactive signals were developed by incubating sections in 3-3’-diaminobenzadine (DAB) reagent (Vector Laboratories). Sections were mounted on glass slides which were serially dehydrated in ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... with 3% donkey serum (Vector Labs), 0.5% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3’-diaminobenzidine (DAB) kit (Vector Laboratories). The percentage of positive cells was rated per high-power field (HPF ...
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bioRxiv - Cancer Biology 2021Quote: ... 3’-diaminobezine detection kit (Vector Laboratories) as previously described 23 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 -diaminobenzidine (DAB) kit (Vector Labs) was applied to visualize the signal ...
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bioRxiv - Neuroscience 2023Quote: ... 3’-diaminobenzidine tetrahydrochloride (DAB; Vector Labs). Immunostained sections were subsequently mounted on Superfrost plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 3’-diaminobenzidine tetrahydrochloride (DAB; Vector Labs), sections were mounted on Superfrost plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 3’-diaminobenzidine tetrahydrochloride (DAB; Vector Labs), sections were mounted on Superfrost plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 3’-diaminobenzidine tetrahydrochloride (DAB; Vector Labs), sections were mounted on Superfrost Plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 3’-diaminobenzidine tetrahydrochloride (DAB; Vector Labs), sections were mounted on Superfrost plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 3’-diaminobenzidine (DAB kit; Vector Laboratories). After placing the immunostained tissues on glass slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody detection was performed using a 3’,3’-diaminobenzidine (DAB) Peroxidase Substrate kit (Vector Laboratories, Burlingame, CA) that resulted in a brown precipitate ...
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bioRxiv - Immunology 2022Quote: ... Rhodamine/Cy-3 coupled lectins (Vector Labs) were diluted in lectin buffer to a concentration of 20ug/ml.
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bioRxiv - Cancer Biology 2024Quote: ... 3′-diaminobenzidine (DAB) (Vector Labs, SK-4105) as substrate according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... 3-amino-9-ethylcarbazole (Vector Laboratories, Inc.) was used as a substrate for visualization ...
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bioRxiv - Neuroscience 2024Quote: ... Successful degradation of PNNs was determined by colorimetric 3-3’-Diaminobenzidine staining with recognition by biotinylated WFA (Vector Laboratories) through use of the VECTASTAIN Elite ABC-HRP avidin-biotin complex (Vector Labs ...