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Citations for Vector Labs :
251 - 300 of 609 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The aldehyde groups resulting in a 40 μl mix were then biotinylated using a mixture containing 4 μl of NaOAc (1M, pH 6.0) and 4 μl of Biotin (Long Arm) Hydrazide (100 mM, catalog num. SP-1100, Vector Laboratories). The 48 μl resulting mixture was next incubated for 30 minutes at 40°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was completed for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...
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bioRxiv - Genetics 2020Quote: ... Sections were incubated with primary antibody (supplementary table S4) overnight at 4°C and with universal biotinylated horse anti-mouse/rabbit immunoglobulin G secondary antibody (Vector Laboratories) for 1 hour ...
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bioRxiv - Physiology 2021Quote: ... Ears were then gently shaken (120 rpm) at 4°C overnight in 20 μg/ml Dylight 488 strepavidin (SA-5488, Vector Laboratories) and 0.05 mg/ml DAPI (D9542 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
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bioRxiv - Physiology 2021Quote: ... Frozen bone sections were incubated for 4 min at room temperature in Alkaline Phosphatase Substrate Solution ImmPACT Vector Red (Vector Laboratories). After washing with buffer ...
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bioRxiv - Microbiology 2021Quote: ... ovaries were fixed in 4% PFA and then mounted in Vectashield® mounting media with DAPI counterstain (Vector Laboratories, Burlingame, CA). Ovaries were then imaged using Differential Interference Contrast on a Zeiss Inverted Observer Z1 at 100X magnification.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 in blocking solution at 4 °C overnight followed by incubation with peroxidase-linked goat anti-rabbit IgG (1:10000, # PI-1000-1, Vector Laboratories). Chemiluminescent substrate (# 34095 ...
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bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
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bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated first with a Biotinylated Goat Anti-Rabbit IgG Antibody (over-night at 4°C-Vector Laboratories), and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... The polyps were kept for at least o/n at 4°C in a Vectashield mounting medium with DAPI (Vector Laboratories) before being mounted and imaged on a Leica SP8 confocal system ...
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bioRxiv - Immunology 2023Quote: ... This was followed by incubation in with biotinylated goat anti-rabbit and donkey anti-guineapig antibodies (Table S5) for 4 h at room temperature then in avidin–biotinylated horseradish peroxidase complex (Elite ABC; 1:300; Vector Laboratories) diluted in PBS for 3 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2% Tween-20 in PHEM buffer and mounted in Vectashield (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an hour in Mouse-on-Mouse (MOM) Blocking Buffer (one drop MOM IgG in 4 ml PBS-T) (Vector Labs). Following the MOM block ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1 hour and then incubated overnight with the primary antibody (Table 1) containing 4 drops/ml of Biotin Solution (of the Avidin/Biotin Blocking Kit, Vector Labs) at RT ...
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bioRxiv - Systems Biology 2024Quote: ... the sections were washed again in PBS-T and incubated in Mouse-on-Mouse (MOM) Blocking Buffer (one drop MOM IgG in 4 ml PBS-T) (Vector Labs) for another hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies for 48 h at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 days at 4°C in avidin-biotinylated horseradish peroxidase complex (Vectastain ABC Elite kit, Vector Laboratories) in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were again washed 4 times with PBS-T followed by addition of avidin D-horseradish peroxidase (HRP) (Vector Laboratories) diluted 1:1,000 in PBS-T with 1% FBS ...
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bioRxiv - Bioengineering 2020Quote: ... beads were stored at 4°C in PBS to reduce background before mounting in VECTASHIELD antifade mounting media (Vector Laboratories H-1000). Images were acquired on a confocal laser scanning microscope (Leica SP5) ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C in blocking solution and biotinylated anti-rabbit IgG secondary antibody (1:3000, Vector Laboratories BA-1000) at RT for 1h ...
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bioRxiv - Biophysics 2021Quote: ... The cells were subsequently fixed in 4% paraformaldehyde for 15 minutes and the coverslips were mounted with Vectashield + DAPI (Vector Laboratories, USA) on a glass slide ...
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bioRxiv - Neuroscience 2022Quote: ... Delipidated sections in PBS were blocked with BSA 4 % and incubated with a 50 ug/ml solution of lectin conjugated with fluorescein (Vector Laboratories, FL1171), 24 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed in 0.01 PBS and incubated overnight at 4 °C with Alexa biotinylated goat anti-rabbit 488 (1:2000, Vector Laboratories, Burlingame, CA). After rinses ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2 % Tween-20 in PHEM buffer and mounted using Vectashield mounting media (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then blocked with 10% goat serum in PBS-Triton 0.1% (T-PBS) containing 4 drops/ml of Avidin solution (of the Avidin/Biotin Blocking Kit, Vector Labs SP-2001) for 1 hour and then incubated overnight with the primary antibody (Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were washed 4 times with PBST and mounted using VectaShield Hardset Antifade mounting medium (Vector Laboratories, Cat#H-1700-10). Images were taken using a Keyence BZ-810 all-in-one fluorescence microscope or a Zeiss LSM 700 inverted confocal microscope.
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bioRxiv - Neuroscience 2024Quote: ... After another washing session (6 × 20 minutes at 4°C) the retinae were embedded in mounting media (Vectashield, Vector Laboratories Inc., USA) on a glass slide ...
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bioRxiv - Developmental Biology 2024Quote: ... De-waxed and rehydrated sections were heated up and then pressure cooked for 4 minutes in antigen unmasking solution (H-3300, Vector Laboratories Inc). Once cooled ...
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fixed with 4% paraformaldehyde and stained with 10 mg/mL rhodamine-labeled Griffonia simplicifolia lectin I (GS-1lectin; Vector Laboratories; Burlingame, CA). Five randomly selected fields were imaged per gel to an area of 3×3 mm and a depth of ∼500 µm using a Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...