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Citations for Vector Labs :
201 - 250 of 1032 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were rinsed 3 times with PBS and mounted on glass slides using Vectorshield antifade mounting medium (H-1000, Vector Laboratories) and cover slipped.
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bioRxiv - Neuroscience 2022Quote: ... The sections were then washed 3 times with 1xPBS for 10 min each and mounted using Vectashield Mounting media (Vector laboratories). The slides were imaged using a Leica SP5 multiphoton confocal microscope and Olympus FluoView FV1000.
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bioRxiv - Bioengineering 2022Quote: ... Slides were then washed 3 times for 10 minutes each in PBS and coverslips were applied with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed 3 times in washing solution and stored in PBS or mounted with coverslips using Vectashield Antifade Mounting Medium (Vector Laboratories).
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... endogenous peroxidase activity was blocked by treating sections with 3% H2O2 and antigens were retrieved with Antigen Unmasking Solution (Vector Laboratories). Tissues were blocked in 10% serum with 0.25% Tween 20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Another 3 x 10 minute washes in PBST were completed before mounting brains on a microscope slide with vectashield (Vector Laboratories) for imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed in PBST-2 for 3 × 20 minutes at room temperature in the dark and mounted on a microscope slide using Vectashield mounting media (Vector Labs). To maintain the 3-dimensional structure of the brain ...
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bioRxiv - Physiology 2024Quote: ... slides were rinsed 3 times in Tris-Buffered Saline (TBS) and then incubated in BLOXALL Endogenous Enzyme Blocking Solution (cat# SP-6000, Vector Laboratories) for 15 min in dark to quench endogenous peroxidase ...
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bioRxiv - Physiology 2024Quote: ... Slides were then dried for 3 min at RT and circled with a Hydrophobic Barrier Pen (Vector Laboratories, GZ-93951-68) before 30 min protein digestion (protease IV ...
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bioRxiv - Microbiology 2024Quote: ... The cysts were washed 3 times with 1x PBS and mounted in VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, Newark, CA). Samples were illuminated using 380nm (CFW) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed 3 times with 1x PBS and mounted in VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, Newark, CA). Samples were illuminated using 380 nm (CFW) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed and stained with biotinylated anti-mouse IgG (Table 3) in PBST 1 % BSA for 1 h at RT before washing and addition of ABC Elite amplification (Vector Labs) for 30 min at RT according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Intestine sections were rehydrated and antigens were unmasked for 30 min in a steamer with sodium citrate (10 mM, pH 6) (Ki67, GFP, p57, PCNA, cleaved caspase 3) or High pH (H-3301, Vector Laboratories) (Olfm4 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Genetics 2022Quote: ... Brains were then washed a final 3 times in PBS and mounted in Vectashield mounting medium (Vector Labs, H-1000-10) for imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then rinsed 3 times for 10 minutes each with PBS and were subsequently treated with DAPI (Vector Labs) at a 1:5,000 dilution for 5 minutes and washed in PBS 3 times ...
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections with PBS (3 x 10 min washes) and incubated the sections with avidin-biotin-peroxidase complex (ABC, ABC Elite Kit, #PK6100, Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Frozen sections were thawed to room temperature and equilibrated for 3 min in PBS before being circled by an ImmEdge hydrophobic pen (Vector Laboratories). Sections were blocked for 45 minutes in 5% BSA in PBS and then incubated with primary antibodies in 5% BSA overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were again washed 3 times (15 minutes) in TBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories) for 2 hours at room temperature before additional washes in TBS (2 times ...
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bioRxiv - Microbiology 2024Quote: ... Preparations were then washed 3 times with PBS to remove excess secondary antibody and the slides were mounted in Vectashield (#H-1000, Vector Laboratories). Images were obtained using a LSM980 Airyscan 2 microscope (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were then rinsed using 1x PBS for 3 times before mounting on glass slides with the Vectorshield antifade mounting medium (H-1000, Vector Laboratories). Slides were left at room temperature overnight before sealing with nail oil and imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed with TBST (3 ×15 min) and mounted on glass slides with VECTASHIELD Vibrance antifade mounting medium with DAPI (Cat# 1800, Vector laboratories).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 days at 4°C in avidin-biotinylated horseradish peroxidase complex (Vectastain ABC Elite kit, Vector Laboratories) in TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Transverse spinal cord and brain sections (30 μm) were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with 3% NDS-PB-TX and first incubated in biotinylated secondary anti-mouse antibody (1:200, Vector Labs, CAMK-IIα amplification) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3x in 0.1M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min ...
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bioRxiv - Cancer Biology 2022Quote: ... in phosphate buffer 0.2 % Triton supplemented with10 % horse serum and rinsed 3 more times 10 min before being mounted in Vectashield (Vector Labs, H-1000). For Phalloidin staining ...
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bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA) followed by Vectastain Elite ABC HRP (Vector labs ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100). Slides were rinsed with water and counterstained with Hematoxylin (RICCA Chemical Company ...
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bioRxiv - Cancer Biology 2021Quote: ... and cuticles were washed 3 times in PBSS before mounting on glass slides in Vectashield mounting media without DAPI (Vector Laboratories, Inc.). Tumours were stained overnight at 4°C with DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed for 3 times in 0.1 M PBS and mounted on glass slides using Vectashield medium containing DAPI (Vector Labs, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections were first washed with a series of PBS and 0.5% PBS-Triton X-100 (PBS-T) before applying a 3% normal goat serum (NGS, #1000, Vector Laboratories, Burlingame, CA) blocking solution for 1 hour at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... For blotting experiments gels were transferred onto nitrocellulose and unspecific binding blocked using 5% BSA and 2% polyvinylpyrrolidone (PVP) for blot detection with α-DAF or biotinylated Sambucus nigra agglutinin (SNA) (Vector Laboratories B-1305-2), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... Nuclei were stained using VECTASHIELD mountant with 5’,6-diamidino-2-phenylindole nuclear stain (DAPI; Vector Laboratories). Samples were then mounted onto glass slides and visualised using an Axiophot microscope or Evos Cell imaging system (Thermo Fischer ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816; secondary: biotinylated goat anti-rabbit IgG, 1:200, 16 minutes; Vector labs, #PK6101), or an anti-c-MYC antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...