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Citations for Vector Labs :
201 - 250 of 2406 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the sections were mounted with hard-set DAPI (4′, 6-diamidino-2-phenylindole –hard set; Cat# H-1500, Vector Laboratories Ltd., Peterborough, UK). The slides were left overnight in the dark at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Physiology 2023Quote: ... The coverslips were washed in PBS before being mounted onto a microscope slide with 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield, Vector Laboratories, Burlingame, CA) mounting medium.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated with the sections for one hour before washing using PBS and mounting using Vectashield Antifade Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2022Quote: ... Cells incubated with MitoSOX™ were then fixed with 3.7% paraformaldehyde and applied with an anti-fading mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Live or fixed cell images were captured at an excitation/emission (in nm ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then cover slipped with Vectashield mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) for nuclear staining (Vector Laboratories, H-1800) and kept at 4L°C until imaging ...
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bioRxiv - Neuroscience 2024Quote: ... and the cover-slips applied with a drop of Vectashield-containing DAPI (4’,6-diamidino-2-phenylinodole; catalog number: H-1200; Vector Laboratories, Burlingame, CA). Sections were visualized on a Zeiss AxioImager M2 microscope equipped with an AxioCam MRm and the ApoTome imaging system ...
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bioRxiv - Immunology 2024Quote: ... diluted in 0.1% Triton X-100 in PBS and then mounted with Vectashield Mounting Medium containing 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA). Confocal microscopy (performed on a Leica TCS SP8 or Leica Stellaris 8 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissue sections were counterstained with methyl green (Vector Laboratories), dehydrated in an ethanol series (95% ...
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bioRxiv - Neuroscience 2020Quote: ... for 24 h at 4°C followed by goat biotinylated anti-rabbit antibody (1:250, Vector Laboratories, BA-1000) for 2h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were incubated overnight at 4°C with Lectin DyLight™ 649 (1:100, Vector Laboratories, DL-1178) and donkey-anti-mouse IgG AF488 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at 4°C in 1:300 biotinylated goat anti-rabbit IgG (Vector Labs, BA-1000) and 1:200 donkey anti-mouse ultrasmall immunogold (Aurion ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... at 4°C overnight and then with an appropriate biotinylated secondary antibody (1:200; Vector Laboratories, Burlingame, CA, USA). The signal was detected with VECTASTAIN Elite ABC-HRP Kit (PK-6100 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in 1:100 biotinylated goat anti-rabbit IgG (Vector Labs, BA-1000) in TBS containing 1% NGS ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 4-6 times over 2 hours in PBT at room temperature and mounted with VECTASHIELD Mounting Media (Vector Labs, Burlingame, CA, USA) using glass slides between two bridge glass coverslips ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each slide was coverslipped using a mounting medium that contained the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA) and stored at −20°C until image acquisition occurred.
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bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).
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bioRxiv - Neuroscience 2024Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight with agitation at 4°C with Wisteria floribunda lectin (WFA, WFL, Biotinylated; Vector Laboratories, Newark, CA, B-1355-2) diluted in staining buffer (2% PBST and 2% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... Cover slips were added and sealed with Vectashield mounting medium plus 4’,6-diamindino-2-phenylindole (DAPI) to visualise nuclei (Vector Laboratories, Burlingame CA, USA). Slides were imaged with an upright confocal microscope (Zeiss LSM880)
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bioRxiv - Neuroscience 2024Quote: ... sections were washed and mounted on slides using a Vectashield mounting medium with 4′,6-diamidino-2-phenyl indole (DAPI) (H-1200; Vector Laboratories, Inc., CA, USA) and a coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with blocking solution (4% normal goat serum [Vector Laboratories, S-2000] in PBS-T [PBS with Triton X-100 0.1%] ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were blocked for 2 to 3 hrs with 2 % heat inactivated goat serum (Vector Laboratories) and 4 mg/ml BSA (AppliChem ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in PBST (3 × 5 min.) and then incubated in 1% ABC solution (Vectastain, Vector Laboratories, Burlingame, CA) and washed again in PBST (3 × 5 min.) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryosections were blocked in PBS containing 10% donkey serum and 0.1% Triton X-100 for 2 h and incubated overnight at 4°C with rabbit anti-Iba-1 and goat anti-Arg1 antibodies or FITC-conjugated Lycopersicon esculentum (Tomato) Lectin (Vector Labs, FL-1171-1). Next ...
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bioRxiv - Developmental Biology 2021Quote: ... and then mounted with Vectashield® mounting media with 4’,6-diamidino-2-phenylindole (DAPI; H-1000 and H-1200; Vector laboratories, Inc, Burlingame, USA). Potential unspecific binding was evaluated by omission of the primary antibody in control sections that were otherwise processed similarly.
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bioRxiv - Neuroscience 2020Quote: ... treated for 1 h with blocking buffer containing 0.3% Triton X-100 and 4% normal horse serum (NHS, Vector Laboratories) prepared in PBS ...
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bioRxiv - Physiology 2022Quote: ... Permeabilized retinas were incubated overnight at 4°C with biotinylated Isolectin B4 (IB4; Vector Laboratories, B-1205, diluted 1:500). Retina were imaged using a Zeiss LSM 780 GaAsp inverted laser scanning fluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were then destained by a gradient of ethanol plus a 2% hydrochloride wash and blocked with extra avidin/biotin (Vector Laboratories, Burlingame, CA, USA) and a Fab fragment block (Jackson Laboratory ...
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bioRxiv - Cancer Biology 2022Quote: ... The slides were then destained by a gradient of ethanol plus a 2% hydrochloride wash and blocked with extra avidin/biotin (Vector Laboratories, Burlingame, CA, USA) and a Fab fragment block (Jackson Laboratory ...
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bioRxiv - Neuroscience 2022Quote: ... The recording electrode (tip filled with 4% Neurobiotin (Vector Laboratories, Burlingame, California) in 1 M potassium chloride ...
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bioRxiv - Pathology 2023Quote: ... mounted using 4′,6-diamidino-2phenylindole (DAPI: Vector Laboratories, Burlingame, CA, USA), and examined employing a laser confocal microscope (LSM 710 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently polyclonal α-PilA antibodies (4) and UEA-fluorescein (Vector Laboratories) were used to detect PilA and HPS respectively following standard protocols (4 ...
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Co-release of GABA and ACh from medial olivocochlear neurons fine tunes cochlear efferent inhibitionbioRxiv - Physiology 2024Quote: ... a second blocking for ∼2 h at room temperature with a Mouse blocker Reagent (2-3 drops in 2.5 ml PBS, MKB-2213-1, Vector Laboratories) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Immunology 2023Quote: ... This was followed by incubation in with biotinylated goat anti-rabbit and donkey anti-guineapig antibodies (Table S5) for 4 h at room temperature then in avidin–biotinylated horseradish peroxidase complex (Elite ABC; 1:300; Vector Laboratories) diluted in PBS for 3 h at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...