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Citations for Vector Labs :
2251 - 2300 of 2309 citations for 5 1 3 Dioxolan 2 yl 2 3 fluorobenzoyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were blocked with BSA in PBS-Tween and antibodies were incubated at the following dilutions: anti-Biotin (1:1000, Vector Labs, SP-3000), anti-RNA polymerase II CTD repeat YSPTSPS [phospho S5] [4H8] (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:30 was applied for 15 minutes followed by biotinylated secondary rabbit anti-rat antibody diluted 1:400 (BA-4001; Vector Laboratories, Burlingame, CA) for 10 minutes and then ready-to-use Bond polymer refine detection kit with 3,3’- diaminobenzidine (DAB ...
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bioRxiv - Pathology 2024Quote: ... antibodies were coadministered overnight and were labeled with horse anti-rabbit or anti-goat Dylight 488 and 594 (Vector Laboratories, 1:300 dilution) secondary antibodies for 30 min ...
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bioRxiv - Pathology 2024Quote: ... followed by incubation with biotinylated secondary antibodies (anti-rabbit or anti-rat, 1:200) and avidin-biotin complex (ABC, Vector Laboratories; CA, USA). Signal was detected using 3,3-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2024Quote: ... or anti- tyrosine hydroxylase antibody (Pel-Freez, 40101, 1:1000) using avidin-biotin complex (ABC) system (Vectastain ABC Elite Kit, Vector Laboratories, Burlingame, CA). The immunocomplexes were visualized with the chromogen 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2024Quote: ... using a primary concentration of 1:200 and a pre-dilute secondary anti-Rb horseradish peroxidase (HRP) polymer (Vector Laboratories, Newark, California, USA) developed using 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2024Quote: ... the brain of mice surviving infection with PruΔMYR1::LucHA::mCherry tachyzoites were homogenised in 1 mL PBS and 300 µL of the sample were stained with FITC-conjugated Dolichos Biflorus Agglutinin (DBA; 1: 200; Vector Laboratories #RL-1031) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed in PBS 1X and incubated for 45 minutes with goat anti-Rabbit IgG secondary biotinylated antibody (1:500, Vector Laboratories, BA-1000) followed by an incubation with Cy3-conjugated streptavidin (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... at a concentration of 1:200 and a fluorescent-conjugated lectin (WFA) which binds carbohydrate structures and excites at 495 nm (Vector Laboratories, cat. # 1351) at a concentration of 2 ng/mL in blocking solution (2 hr ...
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bioRxiv - Neuroscience 2024Quote: ... The next day sections were incubated at room temperature for one hour with a biotinylated secondary antibody (1:200, Vector Laboratories, Burlingame, CA), washed briefly in PBS ...
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Immunology 2024Quote: ... The tissues were counterstained with 1 µg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Fragments of the lung were fixed at room temperature in 2% PFA in PBS (pH = 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... After washing in 0.1% Triton-X-100 in TBS the sections were incubated for one hour at room temperature with Streptavidin Dy Light Alexa antibody (1:500; Vector Labs, Cat. # SA-5488) in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed in 0.5% TX-100 and incubated for 1.5 hours in anti-mouse biotinylated secondary antibody (1:400; Vector Laboratories; cat # BA-9200; RRID: AB_2336171). Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... the brain sections were re-stained the Nb-loaded neuron using the avidin-biotin-horseradish peroxidase complex (Vectastain Elite ABC; Vector Laboratories, 1:200 dilution) with diaminobenzidine and nickel as demonstrated previously [23,54] ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Molecular Biology 2021Quote: ... the slides were incubated with a biotin-conjugated secondary antibody for 1 h and with VECTASTAIN ABC Reagent (Cat. #PK- 4000; Vector Laboratories, Burlingame, CA, USA) for 30 min at 20°C ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... we treated the brain sections with the Fluorescein (FITC) labeled Wisteria Floribunda Lectin (WFA, WFL) (1:500 dilution in 1X PBS, Vector labs, cat#FL-1351) for 2 hours at RT before mounting them in clear mounting medium (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... We incubated free floating sections of the brain in H2O2 (1:50) for 30 minutes then blocked it with 10% Normal Horse Serum (Vector Laboratories, Burlingame, CA, USA) for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... Signal amplification was completed by incubation with streptavidin conjugated with Alexa-Fluor™-488 (1:500 in 0.1% Triton/TBS; Vector Labs, No. SA-5488). Sections were washed in TBS as described above and mounted using aqueous mounting medium containing DAPI (Fluoroshield ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with biotinylated horse anti-mouse IgG secondary antibody (1:200, PK-6102, Vectastain Elite ABC HRP kit, Vector Laboratories, USA, RRID: AB_2336821) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were probed with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature and mounted using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). Images were taken with the LSM 880 confocal microscope (Zeiss).
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bioRxiv - Neuroscience 2023Quote: ... slices were rinsed several times using 100 mM PBS and subsequently incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling slides in a Coplin jar filled with Citrate-Based Antigen Unmasking Solution (1:100 dilution; Vector Laboratories, Cat# H-3300). After cooling to room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Tadpoles are washed with PBT for 1 hour and then washed in PBS for another hour before mounting in Vectashield (Vector Laboratories, Burlingame, CA, USA). Z-stacks were acquired at 1μm for visualizing epidermal MCC’s and 3μm for visualizing entire brain regions.
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... The following day, the sections were incubated with secondary antibodies (1 h, room temperature) and mounted with Vectashield® Mounting Medium (Vector Laboratories, #H-1000). Image acquisition was performed using a Leica SP8 confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed with PBS and incubated for 1 h at room temperature with a protein-blocking solution (VECTASTAIN ABC kit, Vector Laboratories, Burlingame, CA, USA). Excess blocking solution was drained ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on a glass microscope slide using a 4:1 mixture of Citiflour™ AFI mountant solution to Vectashield® vibrance antifade mounting medium (Vector Laboratories, USA). The fluorescence images were taken using an Echo Revolve R4K (Upright & Inverted Capability ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then administered a lethal dose of sedative (sodium pentobarbital) followed by an intravenous injection of DyLight 594-conjugated tomato lectin (100 µl; Vector Laboratories, #DL-1177-1). Mice that received stereotactic surgery were only given injections of tomato lectin (no prior anesthesia/administration of dextran) ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was reduced using endogenous peroxidase and alkaline phosphatase blocking solution (Blockxol, cat# SP-6000) followed by blocking for 1 hr at room temperature using animal-free blocker (Vector Laboratories, cat# SP-5035). Then the sections were stained with primary antibody diluted in SignalStain Antibody Diluent (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were treated with AB solution for 30 min at R/T (Avidin-Biotinylated Horseradish Peroxidase H from Vectastain-ABC KIT of Vector Laboratories 1:200 in PBT).
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bioRxiv - Developmental Biology 2021Quote: Immunohistochemistry was performed as previously described (Frei et al., 2011) using anti-HAND2 antibody (A-12, Sc-398167, 1:100) and secondary universal antibody (Vectastain, Vector laboratories Inc., Burlingame, CA, USA), according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... MA) was used as a marker of hypoxia and lectin (1 mg/kg; DyLight 594 labeled lycopersicon Esculentum tomato, Vector Laboratories, DL-1177, Burlingame, CA) as a marker of ischaemia [17] ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with primary antibody overnight at room temperature and detected using the appropriate biotinylated secondary antibody (1:200, Vector Labs, BA-1100, BA-2000) and alkaline phosphatase-Vector Red according to manufacturer instructions (Vector Labs) ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (40 µm) through the mPFC were sectioned using a sliding microtome and stained with WFA (1:500, Vector Laboratories, FL-1351, RRID AB_2336875) and rabbit anti-PV antibody (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...