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Citations for Vector Labs :
2051 - 2100 of 2119 citations for Rat Angiopoietin 2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and stained with 1:20 diluted fluorescein-conjugated UEA-I (Lectin Kit I, Fluorescein, UEA-I-FL, #FKL2100; Vector Laboratories, Burlingame, CA, USA). The cells were washed with PBS containing 0.2% FBS and subjected to flow cytometry by a Moxi GO II cell analyzer (Orflo ...
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bioRxiv - Neuroscience 2022Quote: ... tissue sections incubated with biotinylated secondary antibody were washed and antigen/antibody complexes were visualized using Vectastain ABS peroxidase kit (Vector Laboratories, Burlingame, CA) followed by counterstaining with Harris hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were again rinsed five times in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by a 30-minute incubation in avidin-biotin-peroxidase complex (Vectastain Elite ABC-HRP Kit #PK-6100, Vector Labs, Burlingame, CA). Detection of antibody signal was revealed with 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 × 10 min with PBS and incubated in a tertiary of avidin and biotinylated horseradish peroxidase (1:1000; ABC kit, Vector Labs, PK-6100) and stained with a 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Chromogenic detection of biotinylated i.v lectin in FPPE tissues was automated on the Leica bond Rx using standard protocol F with the post primary mouse link and secondary detection steps substituted for a streptavidin-biotin-HRP step (Vectastain Elite ABC-HRP Peroxidase kit, Vector labs, #PK-6100).
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bioRxiv - Neuroscience 2024Quote: ... They were then washed three times in PBS and transferred to PBS containing the peroxidase conjugate from the Vectastain CBC kit (Vector Laboratories, Burlingame, CA). After a series of rinses in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... This solution was then removed and tissue slices were incubated in a solution containing the avidin-biotin peroxidase reaction solution (Vectastain Elite ABC Kit, Vector laboratories Inc., USA) for 24 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were processed following ImmPRESS HRP Universal Antibody (horse anti-mouse/rabbit IgG) Polymer Detection Kit and ImmPACT DAB EqV Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the slides were incubated with horse biotinylated anti-mouse/ rabbit IgG ABC-HRP peroxidase at room temperature for 1 hr using the VECTASTAIN Elite ABC Universal Kit (Vector Laboratories, PK-6200). The slides were then washed with 1X PBS for 10 min at room temperature and incubated with VECTASTATIN Elite ABC reagent (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in PBS (5 x 5 minutes) and incubated in avidin-biotin complex with horseradish peroxidase (per manufacturer’s instructions, Vectastain Elite ABC-HRP Kit, Vector Laboratories, Burlingame, CA, USA). After 4 x 5 minute washes in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The immunoreaction products were then visualized according to the avidin-biotin complex method (Hsu et al., 1981) using the Vectastain elite ABC kit (Vector Lab., Burlingame, CA) and 3’,3’-diaminobenzidine (MilliporeSigma ...
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bioRxiv - Pathology 2023Quote: ... 20 Sections were processed following ImmPRESS HRP Universal Antibody (horse anti-mouse/rabbit IgG) Polymer Detection Kit and ImmPACT DAB EqV Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA) protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed with PBS and incubated with the following blocking solutions for 1 hour at room temperature: Mouse Ig Blocking Reagent in Mouse on Mouse (MOM) kit from Vector Laboratories (PK-2200) for staining with antibodies against Cbl (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... The immunoreaction product was then visualized according to the avidin-biotin complex method (Hsu et al., 1981) using the Vectastin elite ABC kit (Vector Lab., Burlingame, CA) and 3’,3’-diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2023Quote: ... The immunoreaction products were then visualized according to the avidin-biotin complex method (Hsu et al., 1981) using the Vectastain elite ABC kit (Vector Lab., Burlingame, CA) and 3’,3’-diaminobenzidine (MilliporeSigma ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibody and the substrate for the detection are added from the Vectastain Universal Elite ABC Immunohistochemistry Kit (Cat # PK-8200, Vector Labs, Burlingame, CA). Slides were incubated in secondary antibody for 20 minutes and washed with PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in a solution containing avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories; 1:50) for 2hrs ...
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... labeled with rabbit anti-ionized calcium-binding adapter molecule (Iba)-1 antibody (Wako Chemicals, Neuss, Germany) and VectaStain Elite ABC-HRP kit (Cat.-No.: PK-6100, Vector Laboratories, Burlingame, USA), and visualized with diaminobenzidine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... a blocking serum is added from the same species from which the secondary antibody is derived (ImmPRESS TM Anti-Rabbit and Anti-Mouse Kit from Vector Labs, Burlington, USA). The blocking was done at room temperature for a minimum of 20 minutes.
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bioRxiv - Cell Biology 2024Quote: ... Cultures were subsequently fixed in 4% PFA and stained for ALP using the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories SK-5300) and Von Kossa using a 4% silver nitrate solution and exposing cultures to 2400 kJ of ultraviolet light using a UV Stratalinker.
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bioRxiv - Microbiology 2021Quote: ... After blocking of endogenous peroxidases sections were incubated with ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Laboratories, MP-7401-15) for 1h at RT and then with DAB substrate (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DIG-labelled probes were visualized using anti-DIG AP-conjugated antibodies and the Vector Red Alkaline Phosphatase (AP) Substrate Kit (Vector Laboratories, Burlingame, CA, US); biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... For staining samples were incubated with PBS + 1/60 Biotin + 1/60 Avidin (VECTASTAIN® Elite® ABC HRP Kit, Vector Laboratories, PK-6100) for 30 min followed by DAB staining (Abcam ...
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bioRxiv - Microbiology 2021Quote: Paraffin-embedded tissues were sectioned at 5 µm and subjected to IHC using Vectastain Elite ABC Peroxidase (HRP) Kit (Vector Laboratories Cat # PK-6102) as previously described [40] ...
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bioRxiv - Pathology 2021Quote: ... we performed immunohistochemical staining using the avidin-biotinylated enzyme complex method and the VECTASTAIN Elite ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, CA, USA). The tissue sections were deparaffinized with xylene and ethanol ...
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bioRxiv - Microbiology 2020Quote: ... stained with anti-E antibody and visualized with a VECTASTAIN Elite ABC anti-mouse IgG kit with a VIP substrate (Vector Laboratories, Burlingame, CA USA). The entire wells of 48-well plates were photographed by Nikon DSLR camera D810 ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were blocked for 20 minutes with two drops of Vector Biotin Block (Avidin/Biotin Blocking kit. SP-2001. Vector Laboratories, Inc. Burlingame, CA). Samples were washed twice with PBS with Protein Block Serum-Free Ready-To-Use for 30 minutes at R.T ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound antibodies was performed with biotinylated secondary goat anti-rabbit antibody and ABC-Kit using the peroxidase substrate method DABImmPact (all from Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with biotin-conjugated anti-rabbit antibody or biotin-conjugated anti-mouse antibody (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, USA). After triple washing steps ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were immerged in 3% H2O2 for 5 minutes to block endogenous peroxidase activity and non-specific binding was blocked with diluted normal horse serum for 20 minutes (Vectastain Universal Elite ABC kit, Vector Laboratories, Burlingame, California, USA). Sections were incubated 1 hour at room temperature with the following primary antibody ...
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bioRxiv - Neuroscience 2019Quote: ... All stainings were visualized using respective secondary antibodies conjugated with HRP or ABC-system (Vectastain Elite ABC HRP Kit, Vector Laboratories Cat# PK-6100) and 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed in PBS and incubated for 1 hour at room temperature in avidin-biotin complex (ABC Elite Kit, 1:500, Vector Laboratories, Burlington, ON, Canada) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplemental Table 1b and the following reagents: ImmPRESS(r) HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50), ImmPRESS(r ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue sections were incubated overnight at 4°C with primary rabbit anti-TH antibodies followed by staining with biotinylated anti-rabbit IgG and an avidin-biotin peroxidase complex (ABC) standard kit (Vector Laboratories, Burlingame, CA, USA). Signals were detected by incubating sections with 0.5 mg/ml 3,3′-diaminobenzidine (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peroxidase-conjugated avidin and the secondary biotinylated antibodies rabbit anti-mouse IgG and rabbit fblanti-goat used in immunohistochemical studies were from VECTASTAIN® Elite ABC-Kit (Vector Laboratories, Burlingame, CA)
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bioRxiv - Pathology 2023Quote: ... the sections were incubated for 30 min with a secondary antibody (peroxidase-labeled anti-mouse/anti-rabbit IgG (ImmPRESS kit, Vector Labs, Burlingame, CA, USA)) ...
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bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Genetics 2023Quote: ... slides were blocked for one hour in 10% goat serum and incubated for one hour at room temperature using a M.O.M kit (Vector Labs Cat# BMK-2202; Newark, CA).
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibody followed by 3,3’-Diaminobenzidine (DAB) staining using the ImmPRESS™ Excel Amplified HRP peroxidase Polymer Staining Kit (Vector Laboratories MP-7602-15), according to the manufacturer’s protocol ...