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Citations for Vector Labs :
2051 - 2094 of 2094 citations for 7 CHLOROTHIENO 2 3 F 1 3 BENZODIOXOLE 6 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Molecular Biology 2021Quote: ... the slides were incubated with a biotin-conjugated secondary antibody for 1 h and with VECTASTAIN ABC Reagent (Cat. #PK- 4000; Vector Laboratories, Burlingame, CA, USA) for 30 min at 20°C ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling slides in a Coplin jar filled with Citrate-Based Antigen Unmasking Solution (1:100 dilution; Vector Laboratories, Cat# H-3300). After cooling to room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Tadpoles are washed with PBT for 1 hour and then washed in PBS for another hour before mounting in Vectashield (Vector Laboratories, Burlingame, CA, USA). Z-stacks were acquired at 1μm for visualizing epidermal MCC’s and 3μm for visualizing entire brain regions.
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bioRxiv - Neuroscience 2022Quote: ... Signal amplification was completed by incubation with streptavidin conjugated with Alexa-Fluor™-488 (1:500 in 0.1% Triton/TBS; Vector Labs, No. SA-5488). Sections were washed in TBS as described above and mounted using aqueous mounting medium containing DAPI (Fluoroshield ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... we treated the brain sections with the Fluorescein (FITC) labeled Wisteria Floribunda Lectin (WFA, WFL) (1:500 dilution in 1X PBS, Vector labs, cat#FL-1351) for 2 hours at RT before mounting them in clear mounting medium (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... We incubated free floating sections of the brain in H2O2 (1:50) for 30 minutes then blocked it with 10% Normal Horse Serum (Vector Laboratories, Burlingame, CA, USA) for one hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were incubated with primary antibody overnight at 4°C then exposed to biotinylated anti-rabbit secondary antibody (Vector Labs, BA-1000, 1:400 dilution, 30Dmin at room temperature followed by avidin-biotin complex formation (Vector Laboratories, # PK-6100), staining with DAB substrate (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Physiology 2023Quote: ... Muscle sections were incubated in 1:1000 Wheat germ agglutinin (WGA– fluorescein 5 μg/mL; Cat# FL-1021-5, Vector Laboratories Ltd., Peterborough, UK) for 10 min followed by three washes of PBS-T for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were probed with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature and mounted using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). Images were taken with the LSM 880 confocal microscope (Zeiss).
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bioRxiv - Neuroscience 2023Quote: ... slices were rinsed several times using 100 mM PBS and subsequently incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with biotinylated horse anti-mouse IgG secondary antibody (1:200, PK-6102, Vectastain Elite ABC HRP kit, Vector Laboratories, USA, RRID: AB_2336821) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was reduced using endogenous peroxidase and alkaline phosphatase blocking solution (Blockxol, cat# SP-6000) followed by blocking for 1 hr at room temperature using animal-free blocker (Vector Laboratories, cat# SP-5035). Then the sections were stained with primary antibody diluted in SignalStain Antibody Diluent (Cell Signaling Technology ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... The following day, the sections were incubated with secondary antibodies (1 h, room temperature) and mounted with Vectashield® Mounting Medium (Vector Laboratories, #H-1000). Image acquisition was performed using a Leica SP8 confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed with PBS and incubated for 1 h at room temperature with a protein-blocking solution (VECTASTAIN ABC kit, Vector Laboratories, Burlingame, CA, USA). Excess blocking solution was drained ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then administered a lethal dose of sedative (sodium pentobarbital) followed by an intravenous injection of DyLight 594-conjugated tomato lectin (100 µl; Vector Laboratories, #DL-1177-1). Mice that received stereotactic surgery were only given injections of tomato lectin (no prior anesthesia/administration of dextran) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were treated with AB solution for 30 min at R/T (Avidin-Biotinylated Horseradish Peroxidase H from Vectastain-ABC KIT of Vector Laboratories 1:200 in PBT).
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bioRxiv - Developmental Biology 2021Quote: Immunohistochemistry was performed as previously described (Frei et al., 2011) using anti-HAND2 antibody (A-12, Sc-398167, 1:100) and secondary universal antibody (Vectastain, Vector laboratories Inc., Burlingame, CA, USA), according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... MA) was used as a marker of hypoxia and lectin (1 mg/kg; DyLight 594 labeled lycopersicon Esculentum tomato, Vector Laboratories, DL-1177, Burlingame, CA) as a marker of ischaemia [17] ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (40 µm) through the mPFC were sectioned using a sliding microtome and stained with WFA (1:500, Vector Laboratories, FL-1351, RRID AB_2336875) and rabbit anti-PV antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with primary antibody overnight at room temperature and detected using the appropriate biotinylated secondary antibody (1:200, Vector Labs, BA-1100, BA-2000) and alkaline phosphatase-Vector Red according to manufacturer instructions (Vector Labs) ...
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bioRxiv - Immunology 2023Quote: Single chain HIV-1 gp140 SOSIP variant strain BG505 [52] was purified over agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5). Column was washed with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Immunohistochemistry for F4/80+ was performed using a rat antimouse F4/80+ antibody (1:100 dilution, AbD Serotec®, Raleigh, NC, USA) subsequently incubated with the appropriate secondary biotinylated antibody (Vector Laboratories Inc., Burlingame, CA, USA) and visualized with Immpact AEC peroxidase (Vector Laboratories Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was then replaced with imaging 2i medium (FluoroBrite DMEM [Thermo Fisher Scientific, A1896701] containing VectaCell Trolox Antifade Reagent for Live Cell Imaging (Vector Laboratories, CB-1000; 1:1,000). After image acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 3 times in PBS and then incubated 1 hour at RT with either ImmPRESS® HRP Horse Anti-Mouse IgG Polymer (Vector Laboratories Cat# MP-7402, RRID:AB_2336528) or ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer (Vector Laboratories Cat# MP-7401 ...
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bioRxiv - Neuroscience 2024Quote: ... as previously described.18 Pan-vascular labeling was achieved through staining with DyLight-594 labeled Lycopersicon Esculentum (Tomato) Lectin (Vector labs, cat. no.: DL-1177-1), which was added to both primary and secondary incubations at 1:100 concentration ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS for 1 h at room temperature and mounted in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories H-1002-10 and H-1000-10). Images used for analysis were captured using a Nikon E800 fluorescent microscope with a 10x objective ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed twice for 20 min in 2XSSC at 42°C and subsequently incubated with fluorescein anti-biotin antibody (Vector Laboratories, SP-3040; Dilution 1:100) for at least 45min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed and incubated for 2h at room temperature in biotinylated horse anti-mouse antibody (1:500, Vector Laboratories Inc., BA-2001, Burlington, ON, Canada). Then ...