Labshake search
Citations for Vector Labs :
1951 - 2000 of 2157 citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The fixed homogenate was stained with a 1:150 dilution of FITC-conjugated Dolichos biflorus agglutinin (Vector Laboratories cat# FL-1031) in 1X PBS and slowly rotated at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... gondii polyclonal antibody (gifted from Fausto Araujo, Palo Alto Medical Foundation, 1:1000) followed by biotinylated goat anti-rabbit IgG antibody (Vector Laboratories). ABC reagent and DAB substrate (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Chambers were first blocked with 1% Pluronic F-127 for 30 min and then with a mixture of 1 mg/mL β-casein and 0.1 mg/mL avidin DN (Vector Laboratories) for at least 30 min ...
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bioRxiv - Physiology 2024Quote: ... then rinsed several times in PB followed by incubation for two hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit PK6100, Vector Laboratories). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Physiology 2023Quote: ... Then the sections were treated in avidin/biotin-peroxidase complex (Vectastain Elite ABC Elite 1:500, Vector Laboratories Inc, Burlingame, US) and the GnRH-immunoreactivity was developed in 0.05% DAB /0.005% H2O2 in 0.05 M Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200; Vectastain standard kit, Vector Laboratories, RRID:AB_2336819). Finally ...
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bioRxiv - Immunology 2024Quote: ... Endogenous peroxidase and biotin were blocked with 1% hydrogen peroxide-methanol for 10 min and a biotin blocking kit (Vector Laboratories), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were washed for three times in TBS and incubated in 10% normal goat serum in TBS-T with a biotinylated secondary antibody from goat against rabbit IgG (1:1000, Vector Laboratories) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS-T and incubated in blocking solution containing biotinylated goat anti-rabbit secondary antibody (1:250, Vector Laboratories) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea) simplicifolia lectin 1 (5 μg/ml) (Vector Laboratories; L-1100). OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα ...
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bioRxiv - Immunology 2022Quote: ... the sections were washed and counterstained with DAPI for 1 min followed by incubation with autofluorescence quencher (Vector Labs #SP-8400). Sections were finally mounted with Vectashield Vibrance Antifade mounting media ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in ABC solution (Peroxidase Standard Vectastain Elite ABC-HRP Kit, Vector Laboratories, Catalog# PK-6100). After 2 x 5min washes in 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... vWAT sections were incubated overnight at 4°C with primary antibody (CD68, 1:100 in PBST) and visualised by probing with Horse Anti-Rabbit IgG Polymer Kit (MP-7401, Vector Labs) for 1 hour at 37°C and ImmPACT DAB Peroxidase Substrate Kit (SK-4105 ...
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bioRxiv - Cell Biology 2023Quote: Vascular perfusion was assessed by injecting 10 μl of DyLight-649 conjugated Lycopersicon Esculentum (Tomato) lectin (DL-1178-1, Vector Laboratories) intracardially (1mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then washed in 1X PBS and counterstained with Hoescht 33342 (1:2000 in 1X PBS) for 10 minutes All samples were then mounted in Vectashield (Vector Labs) and imaged on with a Nikon A1R laser scanning confocal microscope with appropriate filter sets and laser lines.
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at room temperature before the coverslips were washed and finally mounted onto glass slides with Vectashield (Vector Laboratories) mounting solution ...
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bioRxiv - Genetics 2023Quote: ... fixed in 3% paraformaldehyde for 20 min and permeabilised for 1 h with 0.1% triton-X-100 prior to blocking in 2.5% normal horse serum (Vector Laboratories, Peterborough, UK) for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in biotinylated goat anti-rabbit diluted in TBS-X (1:1,000; BA-1000; Vector Laboratories Ltd., Burlingame, USA) for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite ...
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bioRxiv - Cell Biology 2023Quote: ... and differentiation rate was estimated after 96 h by using Dolichos Biflorus Lectin conjugated to Fluorescein (DLB 1:200, FL-1031; Vector Laboratories) to stain the wall of the cysts in formation and α-Sag1 antibody to stain the tachyzoites ...
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bioRxiv - Neuroscience 2023Quote: ... α-d-galactose carbohydrate residues on sensory neurons was visualized using the Bandeiraea simplicifolia isolectin B4 (IB4) conjugated to rhodamine at a 1:250 concentration (RL-1102; Vector Laboratories). Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004 ...
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bioRxiv - Cancer Biology 2022Quote: ... Development of the biotinilated antibody signal was done by 1 h incubation with the Avidin/Biotin-HRP complex (Elite Vectastain ABComplex Kit, Vector Laboratories) following 1 h incubation with Streptavidin-647 (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed (0.1M PBS and 0.1M PB) and incubated with Vectastain Elite ABC-HRP Kit (1:200; Vector Laboratories PK-6100). Sections were developed for 5 min in DAB solution (3,3’diaminobenzidine ...
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bioRxiv - Cell Biology 2023Quote: ... Sections and myofibers that were stained with anti-Pax7 antibody (DSHB) also included a block with 1:50 Mouse on Mouse Ig Blocking Reagent (MKB-2213, Vector Labs).
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bioRxiv - Neuroscience 2023Quote: ... Images were analyzed and edited with ImageJ 2.0.0. (NIH). HRP-labelled sections were revealed (approx. 1 min) using the Diaminobenzidine (DAB) Substrate Kit (Vector Laboratories, SK-4100). The enzymatic reaction was stopped with ddH2O and samples were counterstained with Harry’s hematoxylin (15 s ...
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bioRxiv - Neuroscience 2023Quote: ... BA-2000)/ goat anti-guinea pig (BA-7000)/ goat anti-rabbit (BA-1000) or horse anti-goat (BA-9500) biotinylated secondary antibodies overnight (1:500, Vector Laboratories) followed by extensive washes in TBS (3×10 min ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a biotin-conjugated anti-rabbit or anti-mouse antibody (1:250, Vectastain Elite ABC kit, Vector laboratories). Sections were counterstained with Nissl (0.09% thionin ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with Alexa Flour-conjugated secondary antibody for 1 hr at room temperature and mounted in Vectashield mounting medium containing DAPI (Vector Laboratories). Nikon A1 Confocal microscope (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were washed with PBS (10 min, three times) and incubated with biotin-labeled goat anti-rabbit secondary antibody (1:500; Vector Labs) at room temperature for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by rinsing the sections in PBS and incubating them in a secondary antibody solution (biotinylated anti-rabbit for Arc and DARPP-32 and biotinylated anti-mouse for DCX and TH; 1:200; BA-1000, BA-2000 Vector Laboratories) for 2hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary AlexaFluor conjugated antibodies (ThermoScientific, 1:400) were used to detect isotype-specific primary antibodies and mounted with Vectashield reagent (Vector Laboratories).
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bioRxiv - Neuroscience 2024Quote: ... We then washed the sections three times with PBS (10 min each) and incubated them for 1 hour in avidin-biotin-complex (Vectastain ABC Elite Kit; Vector Laboratories). We washed sections four times in PBS (5 min each ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated with 594 (or 488) labeled secondary antibodies (1:200) and DAPI-containing mounting solution VECTASHIELD (Vector Laboratories). The slices were visualized by using a Nikon inverted microscope Eclipse Ti-U equipped with a digital camera ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... a gift from Dr. Philippe Ciofi) (Griffond, Deray et al. 1993) followed by biotinylated goat anti-rabbit IgG (1:200, Vector Laboratories). Brain sections were further incubated in avidin-biotin peroxidase solution before reacting with Nickel DAB developing solution ...
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bioRxiv - Genomics 2024Quote: Permeabilization with 0.1% Tween-20 in PBS was performed prior to a 1 hr blocking with a blocking solution containing 10% goat serum (Vector Laboratories) and 3% BSA (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... was diluted 1:500 and applied for 30 min at RT followed by biotinylated secondary rabbit anti-goat antibody 1:400 (BA-5000; Vector Laboratories) for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... was carried out according to manufacturer’s protocol after fixation of organoids with 0.1% paraformaldehyde in PBS for 1 hour at room temperature followed by blocking with Avidin/Biotin (Vector Labs SP-2001). Biotin-Maackia Amurensis lectin was labeled with streptavidin-PE conjugate (Thermo Fisher SA10041 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Triton-X-100) four times for 20 min each and kept in Vectashield antifade mounting medium with DAPI (Vector laboratories; H1200), at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... On the following day sections were incubated in biotinylated goat anti-rabbit secondary antibody (1:500, #BA-1000, Vector laboratories, RRID: AB_2313606) and avidin-biotin complex (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was rinsed in TBS-X and reacted with the respective biotinylated secondary antibody (1:200, Vector Laboratories Ltd., Peterborough, UK) for 60 min at room temperature in 1% BSA in TBS-X ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... Rinsing with PBS was followed by incubation in a solution containing biotin-conjugated horse anti-goat IgG (1:200 (Vector Laboratories, USA) in 3 % horse serum + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed 3 times for 5 minutes in 0.1 M PB and placed in goat anti-rabbit OR anti-mouse biotinylated secondary antibodies respectively (1:500, BP-9200/9100; Vector Labs, CA) for 6 hours ...
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bioRxiv - Biochemistry 2021Quote: ... or mouse anti-c-myc (hybridoma supernatant diluted 1:10, clone 9E10) or with biotinylated lectin Canavalia ensiformis agglutinin (ConA) (Vector Laboratories, France) in 1 μg/ml in TTBS (0.05% Tween-20/TBS pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were stained using Wisteria floribunda agglutinin coupled to fluorescein (WFA; 1:100; cat# FL-1351, RRID: AB_2336875, Vector Laboratories, CA, USA) for 12h (at 4°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxide blocking was performed in 1% water for 15 minutes before blocking in 2.5% normal horse serum (Vector Laboratories -MP-7401) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue sections were rinsed again in TS and then incubated in biotinylated WFA (Vector Laboratories, Cat# B-1355, RRID: AB_2336874, 1:50) for 2 h at room temperature ...