Labshake search
Citations for Vector Labs :
1951 - 1990 of 1990 citations for 6 4 methylpiperazin 1 yl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling slides in a Coplin jar filled with Citrate-Based Antigen Unmasking Solution (1:100 dilution; Vector Laboratories, Cat# H-3300). After cooling to room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Tadpoles are washed with PBT for 1 hour and then washed in PBS for another hour before mounting in Vectashield (Vector Laboratories, Burlingame, CA, USA). Z-stacks were acquired at 1μm for visualizing epidermal MCC’s and 3μm for visualizing entire brain regions.
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bioRxiv - Neuroscience 2022Quote: ... Signal amplification was completed by incubation with streptavidin conjugated with Alexa-Fluor™-488 (1:500 in 0.1% Triton/TBS; Vector Labs, No. SA-5488). Sections were washed in TBS as described above and mounted using aqueous mounting medium containing DAPI (Fluoroshield ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... we treated the brain sections with the Fluorescein (FITC) labeled Wisteria Floribunda Lectin (WFA, WFL) (1:500 dilution in 1X PBS, Vector labs, cat#FL-1351) for 2 hours at RT before mounting them in clear mounting medium (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... We incubated free floating sections of the brain in H2O2 (1:50) for 30 minutes then blocked it with 10% Normal Horse Serum (Vector Laboratories, Burlingame, CA, USA) for one hour ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were incubated with primary antibody overnight at 4°C then exposed to biotinylated anti-rabbit secondary antibody (Vector Labs, BA-1000, 1:400 dilution, 30Dmin at room temperature followed by avidin-biotin complex formation (Vector Laboratories, # PK-6100), staining with DAB substrate (Vector Laboratories ...
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bioRxiv - Immunology 2023Quote: ... The brain lysate was 1:10 diluted in the PBS and stained with rhodamine-labeled dolichos biflorus agglutinin (DBA-red, Vector Laboratories, RL-1032-2). GFP and DBA-red double-positive cysts were counted and diluted to 5 cysts per 200 uL of PBS ...
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with biotinylated horse anti-mouse IgG secondary antibody (1:200, PK-6102, Vectastain Elite ABC HRP kit, Vector Laboratories, USA, RRID: AB_2336821) for 30 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Muscle sections were incubated in 1:1000 Wheat germ agglutinin (WGA– fluorescein 5 μg/mL; Cat# FL-1021-5, Vector Laboratories Ltd., Peterborough, UK) for 10 min followed by three washes of PBS-T for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were probed with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature and mounted using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). Images were taken with the LSM 880 confocal microscope (Zeiss).
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bioRxiv - Neuroscience 2023Quote: ... slices were rinsed several times using 100 mM PBS and subsequently incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... The following day, the sections were incubated with secondary antibodies (1 h, room temperature) and mounted with Vectashield® Mounting Medium (Vector Laboratories, #H-1000). Image acquisition was performed using a Leica SP8 confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed with PBS and incubated for 1 h at room temperature with a protein-blocking solution (VECTASTAIN ABC kit, Vector Laboratories, Burlingame, CA, USA). Excess blocking solution was drained ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was reduced using endogenous peroxidase and alkaline phosphatase blocking solution (Blockxol, cat# SP-6000) followed by blocking for 1 hr at room temperature using animal-free blocker (Vector Laboratories, cat# SP-5035). Then the sections were stained with primary antibody diluted in SignalStain Antibody Diluent (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then administered a lethal dose of sedative (sodium pentobarbital) followed by an intravenous injection of DyLight 594-conjugated tomato lectin (100 µl; Vector Laboratories, #DL-1177-1). Mice that received stereotactic surgery were only given injections of tomato lectin (no prior anesthesia/administration of dextran) ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were treated with AB solution for 30 min at R/T (Avidin-Biotinylated Horseradish Peroxidase H from Vectastain-ABC KIT of Vector Laboratories 1:200 in PBT).
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bioRxiv - Developmental Biology 2021Quote: Immunohistochemistry was performed as previously described (Frei et al., 2011) using anti-HAND2 antibody (A-12, Sc-398167, 1:100) and secondary universal antibody (Vectastain, Vector laboratories Inc., Burlingame, CA, USA), according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... MA) was used as a marker of hypoxia and lectin (1 mg/kg; DyLight 594 labeled lycopersicon Esculentum tomato, Vector Laboratories, DL-1177, Burlingame, CA) as a marker of ischaemia [17] ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (40 µm) through the mPFC were sectioned using a sliding microtome and stained with WFA (1:500, Vector Laboratories, FL-1351, RRID AB_2336875) and rabbit anti-PV antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with primary antibody overnight at room temperature and detected using the appropriate biotinylated secondary antibody (1:200, Vector Labs, BA-1100, BA-2000) and alkaline phosphatase-Vector Red according to manufacturer instructions (Vector Labs) ...
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bioRxiv - Immunology 2023Quote: Single chain HIV-1 gp140 SOSIP variant strain BG505 [52] was purified over agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5). Column was washed with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Immunohistochemistry for F4/80+ was performed using a rat antimouse F4/80+ antibody (1:100 dilution, AbD Serotec®, Raleigh, NC, USA) subsequently incubated with the appropriate secondary biotinylated antibody (Vector Laboratories Inc., Burlingame, CA, USA) and visualized with Immpact AEC peroxidase (Vector Laboratories Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was then replaced with imaging 2i medium (FluoroBrite DMEM [Thermo Fisher Scientific, A1896701] containing VectaCell Trolox Antifade Reagent for Live Cell Imaging (Vector Laboratories, CB-1000; 1:1,000). After image acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 3 times in PBS and then incubated 1 hour at RT with either ImmPRESS® HRP Horse Anti-Mouse IgG Polymer (Vector Laboratories Cat# MP-7402, RRID:AB_2336528) or ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer (Vector Laboratories Cat# MP-7401 ...
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bioRxiv - Neuroscience 2024Quote: ... as previously described.18 Pan-vascular labeling was achieved through staining with DyLight-594 labeled Lycopersicon Esculentum (Tomato) Lectin (Vector labs, cat. no.: DL-1177-1), which was added to both primary and secondary incubations at 1:100 concentration ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS for 1 h at room temperature and mounted in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories H-1002-10 and H-1000-10). Images used for analysis were captured using a Nikon E800 fluorescent microscope with a 10x objective ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed twice for 20 min in 2XSSC at 42°C and subsequently incubated with fluorescein anti-biotin antibody (Vector Laboratories, SP-3040; Dilution 1:100) for at least 45min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed and incubated for 2h at room temperature in biotinylated horse anti-mouse antibody (1:500, Vector Laboratories Inc., BA-2001, Burlington, ON, Canada). Then ...