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Citations for Vector Labs :
151 - 200 of 259 citations for Tumor necrosis factor receptor superfamily member 3 TNFRSF3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... before incubating overnight at room temperature in 0.1 M Tris-HCl (pH 9.5) containing nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate at the recommended concentrations (Vector Laboratories). After washing in PBS and postfixing in 4% PFA ...
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bioRxiv - Cell Biology 2020Quote: ... the slide was rinsed with ice-cold ethanol/acetic acid (3:1) solution and mounted with DAPI solution (Vector Laboratories).
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed again in 1X PBS-TX (0.1% Triton X-100) at RT (3×30min) and mounted in Vectashield (Vector Labs) under #1.5 coverslips and imaged.
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bioRxiv - Cancer Biology 2020Quote: ... followed by 3-4 washes with PBST and mounting in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200). Images from two biologically independent experiments were captured at the same time using Zeiss Axio Widefield (20x/0.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase activity was quenched with 3% hydrogen peroxide and slides were incubated in blocking buffer (Vector Laboratories, S-2012) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed for 3 times with 1xPBS (10 min each) and mounted with Vectashield mounting media (Vector laboratories). All mounted slices were imaged using a Leica SP5 multi-photon microscope ...
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bioRxiv - Physiology 2019Quote: ... For immunocytochemistry macrophages fixed with 4% paraformaldehyde and permeabilized with 0.1% PBS-Triton X-100 were blocked with 3% Bovine Serum Albumin (BSA) plus 10% Normal Goat Serum (Vector Laboratories). Samples were probed with a primary antibody against MCT4 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... washed 3 times with PBS for 5 minutes and mounted to a glass slide in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Sections blocked for 30 mins in TBS-T (Trizma buffer saline with 0.1 % Triton X) with 3 % normal goat serum (Vector Laboratories). Sections were incubated with primary antibody rabbit anti c-Fos (1:4000 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed 3 times in PBST and incubated with biotinylated secondary antibodies in blocking buffer (1: 50, Vector Laboratories) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... endogenous peroxidase activity was blocked with a 3% H2O2 solution (VECTOR Antigen Unmasking Solution Citrate-based pH 6.0 H-3300, Vector labs) prior to blocking with 5% goat serum in TBS for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... the fibers were washed 3×10 min in PBS and mounted on glass slides in Vectashield (H-1000, Vector Laboratories) or imaged directly from the glass bottom dish ...
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bioRxiv - Molecular Biology 2022Quote: ... and the glass coverslips were fixed on glass slides with 3 µL of VECTASHIELD (Vector Laboratories, Burlingame, CA, United States).
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bioRxiv - Molecular Biology 2022Quote: ... Samples were stained with DAPI (1μg/ml) for 10 minutes then washed 3 x 5min with PBS and mounted in 2μl of Vectashield (Vector labs). Samples were kept at -20°C until imaged.
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in PBST (3 × 5 min.) and then incubated in 1% ABC solution (Vectastain, Vector Laboratories, Burlingame, CA) and washed again in PBST (3 × 5 min.) ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then washed 3 times with 0.5% Tween-20 before mounting onto glass slides using VECTASHEILD antifade mounting medium with DAPI (Vector Laboratories). Visualisation was done using a fluorescence microscope (Zeiss AxioObserver Z1).
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bioRxiv - Microbiology 2023Quote: ... for 20 minutes at room temperature followed by 3 × 5 minutes washing in PBS (pH 7.4) and then adding Nova Red Peroxide substrate for 10 minutes (Vector laboratories Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... Protein levels were determined by incubating the membrane with ClpL-specific antibodies (1:10,000 in TBS-T + 3% (w/v) bovine serum albumin) and an anti-rabbit alkaline phosphatase conjugate (Vector Laboratories) as the secondary antibody (1:20,000) ...
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bioRxiv - Neuroscience 2024Quote: ... Endogenous enzyme activity was quenched in either methanol containing 3% H2O2 or Bloxall solution (SP-6000-100, Vector Laboratories, US) to block both peroxidase and alkaline phosphate activity ...
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bioRxiv - Genetics 2024Quote: ... blocked with 5% BSA plus 3% goat serum in PBS followed by a blocking with M.O.M.® (Mouse on Mouse) Blocking Reagent (Vector Laboratories), and incubated overnight at 4°C with anti-Pax7 and anti-laminin antibodies (Supplementary Table 6) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a biotinylated anti-human IgG in TBST with 1% goat serum also incubated for 30 minutes at room temperature (Vector Labs, Burlingame, CA). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed 3 times with cold PBS and resuspended in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Cells were imaged at 100X magnification using a Leica DM IRBE microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS for 3 times and mounted with VECTASHIELD antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, H-1200; Vector Laboratories). Images were taken by SP8 confocal microscopy (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour at room temperature in 3% normal donkey serum (NDS, Vector Laboratories, Inc., Burlingame, CA, USA) and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Immunology 2022Quote: ... On day 3 secondary antibodies were added and visualised with the Vector Red Alkaline Phosphatase Substrate Kit (Vector Laboratories #SK-5100). For neutrophil elastase (NE ...
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bioRxiv - Cell Biology 2019Quote: ... The slide was then rinsed by ice-cold ethanol/acetic acid (3:1) and mounted in Vectashield with DAPI (Vector Laboratories) to observe the DNA.
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bioRxiv - Genomics 2019Quote: ... The samples were washed again 3 times as mentioned above before mounting the eye-antennal discs on slide with Vectashield (Vector Laboratories). For imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated for 5 hours at room temperature in VectaFluor DyLight 594 anti-mouse secondary antibody (3 drops in 5mL of 0.1% BSA, DK-8818; Vector Labs, CA). When incubation was completed ...
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bioRxiv - Neuroscience 2021Quote: ... and later in 0.1 M PB (3 × 20 min) and mounted on glass slides with Vectashield mounting medium (Vector Lab, Inc.). The characterizations of primary antibodies used in humans ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed 3 times in PBS and the secondary antibody (biotinylated horse anti-rabbit; 1:250, Vector Laboratories, Burlingame, CA) was added and slides were incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were rinsed 3 times with PBS and mounted on glass slides using Vectorshield antifade mounting medium (H-1000, Vector Laboratories) and cover slipped.
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bioRxiv - Neuroscience 2022Quote: ... The sections were then washed 3 times with 1xPBS for 10 min each and mounted using Vectashield Mounting media (Vector laboratories). The slides were imaged using a Leica SP5 multiphoton confocal microscope and Olympus FluoView FV1000.
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bioRxiv - Bioengineering 2022Quote: ... Slides were then washed 3 times for 10 minutes each in PBS and coverslips were applied with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were washed 3 times for 5 min with PBS-T and incubated with 60 μg/ml PSA-FITC (FL 1051, Vector Laboratories) in PBS for 30 min in dark at room temperature for acrosome labelling ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS three times for 5 min each before being placed in a blocking solution containing 3% normal goat serum (Vector Laboratories) for 1 hr ...
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bioRxiv - Physiology 2019Quote: ... at a dilution of 1:25 for 20 min using agitation followed by 3 washes with PBT and 1 wash in PBS before mounting in Vectashield with DAPI (#H-1200, Vector Laboratories). Analysis of the tissue and image acquisition was carried out using a Zeiss LSM 710 confocal laserscanning microscope and processed using Fiji and Adobe Photoshop.
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed 3 times in washing solution and stored in PBS or mounted with coverslips using Vectashield Antifade Mounting Medium (Vector Laboratories).
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... endogenous peroxidase activity was blocked by treating sections with 3% H2O2 and antigens were retrieved with Antigen Unmasking Solution (Vector Laboratories). Tissues were blocked in 10% serum with 0.25% Tween 20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed and stained with biotinylated anti-mouse IgG (Table 3) in PBST 1 % BSA for 1 h at RT before washing and addition of ABC Elite amplification (Vector Labs) for 30 min at RT according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed an additional 3 × 5 minutes with PBS before adding mounting medium containing DAPI counterstain (Vector labs, H-1200) and applying glass coverslips ...
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bioRxiv - Cell Biology 2022Quote: ... Intestine sections were rehydrated and antigens were unmasked for 30 min in a steamer with sodium citrate (10 mM, pH 6) (Ki67, GFP, p57, PCNA, cleaved caspase 3) or High pH (H-3301, Vector Laboratories) (Olfm4 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Another 3 x 10 minute washes in PBST were completed before mounting brains on a microscope slide with vectashield (Vector Laboratories) for imaging ...
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bioRxiv - Genetics 2022Quote: ... Brains were then washed a final 3 times in PBS and mounted in Vectashield mounting medium (Vector Labs, H-1000-10) for imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then rinsed 3 times for 10 minutes each with PBS and were subsequently treated with DAPI (Vector Labs) at a 1:5,000 dilution for 5 minutes and washed in PBS 3 times ...