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Citations for Vector Labs :
151 - 200 of 259 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Protein levels were determined by incubating the membrane with ClpL-specific antibodies (1:10,000 in TBS-T + 3% (w/v) bovine serum albumin) and an anti-rabbit alkaline phosphatase conjugate (Vector Laboratories) as the secondary antibody (1:20,000) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections that contained the MLPO region (or rostrocaudal series of sections for the projection mapping study) were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times for 15min in 0.02M PBS and mounted between slide and coverslip with Vectashield medium containing DAPI (Vector Laboratories). Negative controls ...
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Co-release of GABA and ACh from medial olivocochlear neurons fine tunes cochlear efferent inhibitionbioRxiv - Physiology 2024Quote: ... a second blocking for ∼2 h at room temperature with a Mouse blocker Reagent (2-3 drops in 2.5 ml PBS, MKB-2213-1, Vector Laboratories) was used ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed 3 times with cold PBS and resuspended in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Cells were imaged at 100X magnification using a Leica DM IRBE microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS for 3 times and mounted with VECTASHIELD antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, H-1200; Vector Laboratories). Images were taken by SP8 confocal microscopy (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour at room temperature in 3% normal donkey serum (NDS, Vector Laboratories, Inc., Burlingame, CA, USA) and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Immunology 2022Quote: ... On day 3 secondary antibodies were added and visualised with the Vector Red Alkaline Phosphatase Substrate Kit (Vector Laboratories #SK-5100). For neutrophil elastase (NE ...
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated for 5 hours at room temperature in VectaFluor DyLight 594 anti-mouse secondary antibody (3 drops in 5mL of 0.1% BSA, DK-8818; Vector Labs, CA). When incubation was completed ...
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bioRxiv - Neuroscience 2021Quote: ... and later in 0.1 M PB (3 × 20 min) and mounted on glass slides with Vectashield mounting medium (Vector Lab, Inc.). The characterizations of primary antibodies used in humans ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed 3 times in PBS and the secondary antibody (biotinylated horse anti-rabbit; 1:250, Vector Laboratories, Burlingame, CA) was added and slides were incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were rinsed 3 times with PBS and mounted on glass slides using Vectorshield antifade mounting medium (H-1000, Vector Laboratories) and cover slipped.
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bioRxiv - Neuroscience 2022Quote: ... The sections were then washed 3 times with 1xPBS for 10 min each and mounted using Vectashield Mounting media (Vector laboratories). The slides were imaged using a Leica SP5 multiphoton confocal microscope and Olympus FluoView FV1000.
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bioRxiv - Bioengineering 2022Quote: ... Slides were then washed 3 times for 10 minutes each in PBS and coverslips were applied with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were washed 3 times for 5 min with PBS-T and incubated with 60 μg/ml PSA-FITC (FL 1051, Vector Laboratories) in PBS for 30 min in dark at room temperature for acrosome labelling ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS three times for 5 min each before being placed in a blocking solution containing 3% normal goat serum (Vector Laboratories) for 1 hr ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed 3 times in washing solution and stored in PBS or mounted with coverslips using Vectashield Antifade Mounting Medium (Vector Laboratories).
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... endogenous peroxidase activity was blocked by treating sections with 3% H2O2 and antigens were retrieved with Antigen Unmasking Solution (Vector Laboratories). Tissues were blocked in 10% serum with 0.25% Tween 20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Another 3 x 10 minute washes in PBST were completed before mounting brains on a microscope slide with vectashield (Vector Laboratories) for imaging ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and mounted onto glass slides with DAPI nuclei stain VECTASHIELD mountant (Vector Laboratories). Samples were visualised on Evos Cell imaging system (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed in PBST-2 for 3 × 20 minutes at room temperature in the dark and mounted on a microscope slide using Vectashield mounting media (Vector Labs). To maintain the 3-dimensional structure of the brain ...
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bioRxiv - Physiology 2024Quote: ... slides were rinsed 3 times in Tris-Buffered Saline (TBS) and then incubated in BLOXALL Endogenous Enzyme Blocking Solution (cat# SP-6000, Vector Laboratories) for 15 min in dark to quench endogenous peroxidase ...
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bioRxiv - Physiology 2024Quote: ... Slides were then dried for 3 min at RT and circled with a Hydrophobic Barrier Pen (Vector Laboratories, GZ-93951-68) before 30 min protein digestion (protease IV ...
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bioRxiv - Microbiology 2024Quote: ... The cysts were washed 3 times with 1x PBS and mounted in VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, Newark, CA). Samples were illuminated using 380nm (CFW) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed 3 times with 1x PBS and mounted in VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, Newark, CA). Samples were illuminated using 380 nm (CFW) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed and stained with biotinylated anti-mouse IgG (Table 3) in PBST 1 % BSA for 1 h at RT before washing and addition of ABC Elite amplification (Vector Labs) for 30 min at RT according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed an additional 3 × 5 minutes with PBS before adding mounting medium containing DAPI counterstain (Vector labs, H-1200) and applying glass coverslips ...
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bioRxiv - Cell Biology 2022Quote: ... Intestine sections were rehydrated and antigens were unmasked for 30 min in a steamer with sodium citrate (10 mM, pH 6) (Ki67, GFP, p57, PCNA, cleaved caspase 3) or High pH (H-3301, Vector Laboratories) (Olfm4 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Genetics 2022Quote: ... Brains were then washed a final 3 times in PBS and mounted in Vectashield mounting medium (Vector Labs, H-1000-10) for imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then rinsed 3 times for 10 minutes each with PBS and were subsequently treated with DAPI (Vector Labs) at a 1:5,000 dilution for 5 minutes and washed in PBS 3 times ...
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections with PBS (3 x 10 min washes) and incubated the sections with avidin-biotin-peroxidase complex (ABC, ABC Elite Kit, #PK6100, Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Frozen sections were thawed to room temperature and equilibrated for 3 min in PBS before being circled by an ImmEdge hydrophobic pen (Vector Laboratories). Sections were blocked for 45 minutes in 5% BSA in PBS and then incubated with primary antibodies in 5% BSA overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were again washed 3 times (15 minutes) in TBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories) for 2 hours at room temperature before additional washes in TBS (2 times ...
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bioRxiv - Microbiology 2024Quote: ... Preparations were then washed 3 times with PBS to remove excess secondary antibody and the slides were mounted in Vectashield (#H-1000, Vector Laboratories). Images were obtained using a LSM980 Airyscan 2 microscope (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were then rinsed using 1x PBS for 3 times before mounting on glass slides with the Vectorshield antifade mounting medium (H-1000, Vector Laboratories). Slides were left at room temperature overnight before sealing with nail oil and imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed with TBST (3 ×15 min) and mounted on glass slides with VECTASHIELD Vibrance antifade mounting medium with DAPI (Cat# 1800, Vector laboratories).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 days at 4°C in avidin-biotinylated horseradish peroxidase complex (Vectastain ABC Elite kit, Vector Laboratories) in TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Transverse spinal cord and brain sections (30 μm) were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121 ...
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bioRxiv - Neuroscience 2024Quote: ... After 3 washes in PBS cells were incubated with a blocking medium consisting of (5% (w/v) bovine serum albumin (Vector Laboratories) and 0.2% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).
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bioRxiv - Neuroscience 2024Quote: ... sections were washed and mounted on slides using a Vectashield mounting medium with 4′,6-diamidino-2-phenyl indole (DAPI) (H-1200; Vector Laboratories, Inc., CA, USA) and a coverslip ...