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Citations for Vector Labs :
151 - 200 of 1982 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated with the sections for one hour before washing using PBS and mounting using Vectashield Antifade Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2022Quote: ... Cells incubated with MitoSOX™ were then fixed with 3.7% paraformaldehyde and applied with an anti-fading mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Live or fixed cell images were captured at an excitation/emission (in nm ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then cover slipped with Vectashield mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) for nuclear staining (Vector Laboratories, H-1800) and kept at 4L°C until imaging ...
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bioRxiv - Neuroscience 2024Quote: ... and the cover-slips applied with a drop of Vectashield-containing DAPI (4’,6-diamidino-2-phenylinodole; catalog number: H-1200; Vector Laboratories, Burlingame, CA). Sections were visualized on a Zeiss AxioImager M2 microscope equipped with an AxioCam MRm and the ApoTome imaging system ...
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bioRxiv - Immunology 2024Quote: ... diluted in 0.1% Triton X-100 in PBS and then mounted with Vectashield Mounting Medium containing 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA). Confocal microscopy (performed on a Leica TCS SP8 or Leica Stellaris 8 ...
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bioRxiv - Neuroscience 2021Quote: ... before incubating overnight at room temperature in 0.1 M Tris-HCl (pH 9.5) containing nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate at the recommended concentrations (Vector Laboratories). After washing in PBS and postfixing in 4% PFA ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 3-4 washes with PBST and mounting in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200). Images from two biologically independent experiments were captured at the same time using Zeiss Axio Widefield (20x/0.8 ...
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bioRxiv - Neuroscience 2022Quote: ... their tips filled with Neurobiotin tracer (Vector Laboratories; 4 % in 1 mol· l−1 KCl) and their shanks filledwith 1 mol· l−1 KCl ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 4-6 times over 2 hours in PBT at room temperature and mounted with VECTASHIELD Mounting Media (Vector Labs, Burlingame, CA, USA) using glass slides between two bridge glass coverslips ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each slide was coverslipped using a mounting medium that contained the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA) and stored at −20°C until image acquisition occurred.
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bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).
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bioRxiv - Evolutionary Biology 2024Quote: ... Chromosomes and nuclei were stained with 2 ng/µl DAPI (4’,6-diamidino-2-2phenylindole) in Vectashield antifade medium (Vector Laboratories, Burlingame, CA, USA). Preparations were analysed with an Axiolmager M2 epifluorescent microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Neuroscience 2024Quote: ... Cover slips were added and sealed with Vectashield mounting medium plus 4’,6-diamindino-2-phenylindole (DAPI) to visualise nuclei (Vector Laboratories, Burlingame CA, USA). Slides were imaged with an upright confocal microscope (Zeiss LSM880)
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bioRxiv - Neuroscience 2024Quote: ... sections were washed and mounted on slides using a Vectashield mounting medium with 4′,6-diamidino-2-phenyl indole (DAPI) (H-1200; Vector Laboratories, Inc., CA, USA) and a coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... Neurobiotin (6 mM, Vector Labs) was added to the internal pipette solution to locate and reconstruct the recorded neurons in subdivisions of BNST after experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 days at 4°C in avidin-biotinylated horseradish peroxidase complex (Vectastain ABC Elite kit, Vector Laboratories) in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... 3 hours in Fluorescein-Avidin (1:500; Vector Laboratories Cat# A-2001, RRID:AB_2336455) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... incubation for 1 to 3 min and with Hematoxylin (Vector Laboratories, H-3401) incubation for 3 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and then mounted with Vectashield® mounting media with 4’,6-diamidino-2-phenylindole (DAPI; H-1000 and H-1200; Vector laboratories, Inc, Burlingame, USA). Potential unspecific binding was evaluated by omission of the primary antibody in control sections that were otherwise processed similarly.
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bioRxiv - Cell Biology 2020Quote: ... 1 µl stained sample was mixed with 3 µl Vectashield mounting medium (Vector Laboratories) and mounted on a slide ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were stained with DAB substrate (Vector Laboratories SK-4100, using reagents 1-3) for 3 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were developed using ImmPACT-3 3’-diaminobenzidine (DAB; Vector Labs), sequentially dehydrated as previously described (Stoyka et al. ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 3 h in biotinylated horse anti-goat (1:200, Vector Labs) or biotinylated goat anti-rabbit secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI; Vector Laboratories). Each in-situ hybridization included 1 slide for each group ...
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bioRxiv - Cell Biology 2023Quote: ... Citrate-buffer at pH 6 (Vector Laboratories) antigen retrieval method was performed on samples required for collagen 1 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... 6-diamidino-2-phenylindole (DAPI)(Vector Laboratory) on poly-L-lysine coated microscope slides ...
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bioRxiv - Developmental Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Vector Lab).
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-phenylindole (DAPI, Vector Laboratories) and imaged with Zeiss Axio Observer inverted microscope equipped with a Zeiss LSM 800 confocal module (Carl Zeiss Microimaging GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed and stained with biotinylated anti-mouse IgG (Table 3) in PBST 1 % BSA for 1 h at RT before washing and addition of ABC Elite amplification (Vector Labs) for 30 min at RT according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Tissue was blocked for 1 hour at room temperature with 3% normal horse serum (Vector Laboratories) in 1X PBS containing 0.1% bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... 6-diamino-2-phenylindole (DAPI; Vector Laboratories, H1200). Images were acquired using a Zeiss Confocal LSM 700 Laser Scanning confocal microscope using 40X Zeiss Plan-Neofluar 40X/1.3 and 63X/1.4 oil immersion objectives ...
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bioRxiv - Neuroscience 2020Quote: The next day sections were wash 6 × 10 min with wash buffer (1 × TBS, 0.05% triton X-100) and incubated with biotinylated antibody (Vector Laboratories) diluted 1:200 in secondary blocking buffer (1 × TBS pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... β-amyloid was detected using thiazine red (1 µM, Cat# 2150-33-6, Chemsavers) associated with DAPI (0.5 µM, Cat# 1485, Vector lab) and a mouse monoclonal antibody against VSM α-smooth muscle actin (Cat# ABT1487 ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 in blocking solution at 4 °C overnight followed by incubation with peroxidase-linked goat anti-rabbit IgG (1:10000, # PI-1000-1, Vector Laboratories). Chemiluminescent substrate (# 34095 ...
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bioRxiv - Bioengineering 2023Quote: ... Cyanine (Cy)3- and Cy5-conjugated secondary antibodies were used at 1:400 (Vector Laboratories, Burlingame, CA).
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in 3% BSA with mouse-on-mouse kit (Vector Laboratories, MKB-2213-1), and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed 3 times with PBS-T containing 1% BSA and mounted using VectaShield (Vector Labs). DNA fibers were imaged at 60X with a Nikon 90i microscope.
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bioRxiv - Cancer Biology 2022Quote: ... antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816; secondary: biotinylated goat anti-rabbit IgG, 1:200, 16 minutes; Vector labs, #PK6101), or an anti-c-MYC antibody (1:100 ...
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bioRxiv - Immunology 2020Quote: ... 3-diaminobenzidine (DAB) (Vector Laboratories). Mouse spleen ...
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bioRxiv - Pathology 2020Quote: ... 3’-diaminobenzidine (DAB; Vector Laboratories) was used as a substrate according to the manufacturer’s instructions and hematoxylin was used as a counter-stain ...
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bioRxiv - Neuroscience 2022Quote: ... After loading the micropipette with 4% Neurobiotin (Vector Laboratories, Burlingame, UK, dissolved in 1 M KCl), it was filled with a 1 M KCl solution ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4°C and stained with fluorescein-conjugated streptavidin (Vector Laboratories, Peterborough, UK, 1:500). Cells were counterstained with ProLong® Gold Antifade Reagent with DAPI for nuclei staining (Cell Signalling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 4 hours of staining with rhodamine conjugated PNA (1:250, Vector Labs RL 1072) and WGA alexa fluor 488 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... and preblocked for 1 h at 4°C in 5% normal goat serum (NGS; Vector Laboratories), 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoreactive signals were developed by incubating sections in 3-3’-diaminobenzadine (DAB) reagent (Vector Laboratories). Sections were mounted on glass slides which were serially dehydrated in ethanol ...