Labshake search
Citations for Vector Labs :
1901 - 1950 of 1984 citations for 7 Chloro 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the sections were washed off with PBS and the slides were probed with Biotinylated secondary antibody (Cat No. BA-1000, Vector Laboratories, 1:200) and VECTASTAIN® Elite® ABC HRP reagent (Cat No ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times with 0.1% PBST and then incubated for 1 hour at room temperature with ImmPRESS anti-rabbit peroxidase conjugated antibody (Vector Laboratories, Burlingame, CA, USA). Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100) ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Physiology 2020Quote: ... The slides were then incubated for 1 hour in a humidity chamber in a mixture of goat anti-rabbit peroxidase conjugated (Vector Labs PI-1000). and goat anti-chicken Alexafluor 488 conjugated (Abcam #ab150169) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with DyLight 594-conjugated goat anti-rabbit secondary antibody (1:1000, Vector Laboratories DI-1594) for 2 h on a shaker at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with biotinylated goat anti-rabbit secondary antibody (1:600 dilution; Vector Laboratories, catalog #BA-1000) in PBS-Tx and 1% NGS for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories, Burlingame, CA). Slides were developed with Bio-Red (Biopath ...
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... and stained overnight at 4C with FITC-conjugated IB4 (Fluorescein labeled Griffonia Simplicifolia Lectin I isolectin B4, FL-1201, Vector laboratories, 1:200) or primary antibody against Cld-5 (mouse monoclonal ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:200 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500). Imaging was performed on a Leica SP8 Confocal microscope using a 40x oil objective ...
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bioRxiv - Developmental Biology 2022Quote: ... Ki67 staining was performed using monoclonal rabbit anti-Ki67 antibody (1:100, Linaris, Dossenheim, Germany) and signals were amplified by the use of the Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Excess antibody buffer was removed with PBSTx and followed by incubation in biotinylated goat anti-mouse secondary antibody (1:1000 Cat# BP-9200-50: Vector Laboratories, California USA) overnight at 4°C in PBSTx and 1% NGS ...
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bioRxiv - Microbiology 2022Quote: ... brain sections were stained with rabbit anti-SINV capsid antibody (1:5000 dilution) and the Vectastain Elite ABC HRP kit (Vector Laboratories, PK-6101) was used for signal detection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and stained with 1:20 diluted fluorescein-conjugated UEA-I (Lectin Kit I, Fluorescein, UEA-I-FL, #FKL2100; Vector Laboratories, Burlingame, CA, USA). The cells were washed with PBS containing 0.2% FBS and subjected to flow cytometry by a Moxi GO II cell analyzer (Orflo ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody incubation was with horse anti-mouse IgG conjugated to biotin (1:100 in PBS; Vector Labs, catalog BA-2000-1.5) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 × 10 min with PBS and incubated in a tertiary of avidin and biotinylated horseradish peroxidase (1:1000; ABC kit, Vector Labs, PK-6100) and stained with a 3 ...
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bioRxiv - Neuroscience 2022Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Cancer Biology 2022Quote: ... CDX tumors were grown to approximately 750 mm3 and mice received an i.v injection of Biotinylated Tomato Lectin (4mg/kg, Vector Laboratories, B-1175-1) one hour before they were sacrificed by Schedule 1 method ...
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bioRxiv - Neuroscience 2023Quote: ... or the corresponding secondary antibodies: biotinylated goat anti-guinea pig antibody (1:100 dilution for GFP detection; Vector Laboratories, Cat# PK-4007; RRID:AB_2336816) and secondary antibody goat anti-mouse IgG coupled to 1.4 nm gold (1:100 dilution for TH detection ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed with PBS and incubated with the following blocking solutions for 1 hour at room temperature: Mouse Ig Blocking Reagent in Mouse on Mouse (MOM) kit from Vector Laboratories (PK-2200) for staining with antibodies against Cbl (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... were used as described previously (Kantar et al., 2021) for 1 hour at room temperature before mounting the coverslips with Vectashield (Vector Laboratories #H-1200) and imaging on Zeiss Apotome or Leica Thunder microscopes.
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with anti-mouse Ig-fluorescein antibody (1:200) and mounted on glass slides using DAPI (Vectashield, Vector Laboratories, CA, USA). Images were acquired in 40X magnification using a fluorescence microscope BX53 (Olympus Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in a solution containing avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories; 1:50) for 2hrs ...
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were blocked with BSA in PBS-Tween and antibodies were incubated at the following dilutions: anti-Biotin (1:1000, Vector Labs, SP-3000), anti-RNA polymerase II CTD repeat YSPTSPS [phospho S5] [4H8] (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... staining to visualize activated microglia was performed as previously described [48] using Iba1 primary antibody (Wako, 1:500) with Vectastain Rabbit Elite ABC-HRT Kit (Vector Labs, PK-6101). Second ...
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bioRxiv - Neuroscience 2023Quote: ... labeled with rabbit anti-ionized calcium-binding adapter molecule (Iba)-1 antibody (Wako Chemicals, Neuss, Germany) and VectaStain Elite ABC-HRP kit (Cat.-No.: PK-6100, Vector Laboratories, Burlingame, USA), and visualized with diaminobenzidine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at RT and initiation of color reaction with a Peroxidase Substrate Kit (SG Peroxidase Substrate Kit, Vector Laboratories, SK-4700). All stained sections were mounted ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed and then incubated in secondary antibody (Alexa 488) for 1 h at room temperature and mounted using VECTASHIELD with DAPI (VECTOR Laboratories Ltd, UK) and coverslips.
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bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-conjugated anti-goat or anti-rabbit secondary antibodies were each applied at 1:500 dilution and incubated with Vector elite Reagent (Vector Laboratories, CA, USA) for 45 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies with their respective concentrations were as follows Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (1:25, Vector Laboratories, #FL-1201), mouse monoclonal anti-3G5 IgM (1:10 ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used for immunohistochemistry included biotinylated goat anti-rabbit (BA-1000) and biotinylated horse anti-goat (BA-9500) at a concentration of 1:200 (Vector laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times in PBS and 2 times in 0.1 M phosphate buffer solution (10 min/wash) before being mounted and cover-slipped using Vectashield Vibrance w/DAPI (Vector Laboratories H-1800). Fluorescent conjugates of streptavidin were stored and prepared as recommended by the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... The tissues were counterstained with 1 μg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Stained tissues were imaged on confocal Zeiss 710 microscope operated by ZEN software (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2024Quote: ... at 1:500 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500).
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bioRxiv - Cell Biology 2024Quote: ... the slides were incubated with horse biotinylated anti-mouse/ rabbit IgG ABC-HRP peroxidase at room temperature for 1 hr using the VECTASTAIN Elite ABC Universal Kit (Vector Laboratories, PK-6200). The slides were then washed with 1X PBS for 10 min at room temperature and incubated with VECTASTATIN Elite ABC reagent (Vector Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Neuroscience 2019Quote: ... The sections of cerebellum were incubated with monoclonal mouse antibodies against HuCD (ICN, Biomedicals, United States) (1:300) and glutamine synthetase (Vector Laboratories Burlingame, United States) (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... After washing in 0.1% Triton-X-100 in TBS the sections were incubated for one hour at room temperature with Streptavidin Dy Light Alexa antibody (1:500; Vector Labs, Cat. # SA-5488) in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed in 0.5% TX-100 and incubated for 1.5 hours in anti-mouse biotinylated secondary antibody (1:400; Vector Laboratories; cat # BA-9200; RRID: AB_2336171). Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... the brain sections were re-stained the Nb-loaded neuron using the avidin-biotin-horseradish peroxidase complex (Vectastain Elite ABC; Vector Laboratories, 1:200 dilution) with diaminobenzidine and nickel as demonstrated previously [23,54] ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...