Labshake search
Citations for Vector Labs :
1801 - 1850 of 1918 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
-
bioRxiv - Neuroscience 2024Quote: ... Immunohistochemistry was performed on free-floating sections using a standard avidin-biotin (ABC Standard Elite kit, Vector Laboratories, Burlingame, CA, USA) method ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200; Vectastain standard kit, Vector Laboratories, RRID:AB_2336819). Finally ...
-
bioRxiv - Physiology 2024Quote: ... then rinsed several times in PB followed by incubation for two hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit PK6100, Vector Laboratories). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
-
bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibody double labelling was through an ImmPress Duet double staining anti-mouse HRP (brown)/anti-rabbit AP (magenta) kit (MP7724, Vector Laboratories, UK) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... the sections were subjected to the staining protocol of the Vectastain Universal Elite ABC Kit (PK-6200, Vector Laboratories, Inc., Burlingame, CA). Finally ...
-
bioRxiv - Cancer Biology 2020Quote: ... unspecific bindings (5% BSA, 5% NGS in PBST, 1h) and Avidin/Biotin (Avidin/Biotin Blocking Kit, Vector Laboratories, SP-2001, 15 min). Samples were incubated overnight at 4°C with anti-MDA antibody (Abcam ...
-
bioRxiv - Neuroscience 2020Quote: ... and BrdU (mice, AbD Serotec) were detected with biotinylated secondary antibodies (Jackson) and the Vector-Elite ABC kit (Vector Laboratories, Burlingame, CA). Finally ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... Another PBS/TWEEN washing battery was performed and sections were then incubated in an avidin-biotin-peroxidase complex (ABC kit, Vector Laboratories®) for 2 h ...
-
bioRxiv - Neuroscience 2020Quote: ... Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody (ImmPRESS Reagent Kit, MP-7401, Vector Laboratories, Germany) with AEC+ (K346 ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
-
bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
-
bioRxiv - Physiology 2021Quote: ... the sections were immunostained by the avidin–biotin peroxidase method using the Vectastain Elite Kit (PK-6102, Vector Laboratories, Burlingame, CA, USA) with 3–3-diaminobenzidine and hydrogen peroxide as the chromogen ...
-
bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
-
bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by boiling for 20 min in a 0.01 M sodium citrate (pH 6.0) solution and endogenous biotin was blocked using the Avidin/Biotin Blocking Kit (Vector Labs, SP-2001). Slides were incubated overnight with primary antibodies against anti-rabbit H3K4me3 (Cell Signaling Technology ...
-
Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Non-specific binding sites were blocked for 1 hour (Col2: MoM Kit, Vector Labs, BMK-2202; Sox9: 10% v/v goat serum). Primary antibodies were incubated overnight at 4°C and included Col2 (1/100 ...
-
bioRxiv - Neuroscience 2021Quote: ... Following, the secondary antibody (The ImmPRESS Universal Antibody Kit, anti-mouse/rabbit) and ImmPACT DAB (both purchased from Vector Laboratories, Burlingame, CA) was applied according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Chromogen development was then conducted with HRP 3,3’-Diaminobenzidine (DAB) or Vector Blue kits (Vector Laboratories, Cat # SK-4100 or SK-5300). Chromogen development was halted by submersion in tap water ...
-
bioRxiv - Cell Biology 2021Quote: ... before peroxidase staining was conducted for 30 min with a peroxidase staining kit (VECTASTAIN® ABC Reagent, cat # PK-4000, Vector Laboratories) as described by the manufacturer ...
-
bioRxiv - Immunology 2020Quote: ... and nonspecific epitopes (species-specific serum diluted 1:5 in Tris-buffered saline and 5% bovine serum albumin, or blocking serum from species specific ImmPRESS® kit; Vector Laboratories) and incubated with primary antibody (Table 2 ...
-
Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and finally an avidin-biotin-peroxidase complex (ABC kit standard, PK-6100, Vectastain, Vector Laboratories, Burlingame, CA, USA; 1:250; 1 h). Peroxidase activity was detected using a solution containing 0.02 % 3,3’-diaminobenzidine tetrahydrochloride ...
-
bioRxiv - Neuroscience 2023Quote: ... non-specific binding was blocked for 30 minutes using 2.5% normal horse serum from the ImmPRESS Anti-Mouse IgG / Anti-rabbit IgG Reagent Kit (Vector Laboratories, CA, USA). (iii ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and blocked with 10% goat or rabbit serum in PBS for 60 min and with Avidin/Biotin Blocking Kit (Vector Laboratories, Inc.) for 15 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by blocking of nonspecific binding sites using normal goat serum (10%) and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA). Sections were incubated with phospho-SMAD2/3 (Cell Signaling Technology ...
-
bioRxiv - Immunology 2023Quote: ... and anti-mouse βIII Tubulin (Table S2) was revealed using a mouse-on-mouse kit according to manufacturer’s instructions (Vector laboratories, Burlingame, CA).
-
bioRxiv - Cell Biology 2022Quote: ... Engineered cartilage sheet sections were also treated with pronase and primary anti-Collagen Type II (DSHB II-II6B3) followed by VectaFluor R.T.U Antibody Kit DyLight® 488 (Vector Labs DI-2788) following manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG, Vector Laboratories, PK-4004). Antibody binding was visualized with DAB-nickel (VWR ...
-
bioRxiv - Physiology 2023Quote: ... Another block was applied with streptavidin and then biotin solutions (Streptavidin/Biotin Blocking Kit, Vector Labs; Newark, CA, USA; Cat. No.: SP2002) at room temperature for 15 min each ...
-
bioRxiv - Neuroscience 2023Quote: ... Brain slices were incubated with 1% H2O2 to block endogenous peroxidase and then with the appropriate biotinylated secondary antibody using the VECTASTAIN-ABC kit (Vector Laboratories Inc.), following the manufactureŕs instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibody binding and 3,3’-diaminobenzidine staining was performed according to the manufacturer’s instruction (Vectastain Elite ABC Kit, Vector Labs, Peterborough, United Kingdom). In brief ...
-
bioRxiv - Neuroscience 2024Quote: ... The Aβ 42/40 double-labelled samples were incubated in ImmPRESS horseradish peroxidase polymer then ImmPRESS alkaline phosphate polymer kits for 30 mins each (Vector Laboratories, US). For these sections ...
-
bioRxiv - Systems Biology 2024Quote: ... Sections were rinsed once more in 0.1% Triton-X in TBS then incubated in an avidin-biotin complex (PK-6100; Vectastain ABC Elite kit, Vector Laboratories, Newark, CA) for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: Slices containing biocytin fills were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer overnight and processed with Vectastain ABC (avidin-biotinylated HRP complex) Elite Kit (Vector Labs, Burlingame, CA). The shape of the slice ...
-
bioRxiv - Cell Biology 2020Quote: Differentiated iBAs cells transfected with a plasmid containing Them1 linked to APEX2 were fixed and the APEX2 developed using an ImPACT peroxidase substrate kit (Vector Labs, Burlingame, CA). Cells were then frozen using a Wohlwend Compact 02 High Pressure Freezer ...
-
bioRxiv - Neuroscience 2021Quote: ... The immunoreaction products were then be visualized according to the avidin-biotin complex method (Hsu et al., 1981) using the Vectastin elite ABC kit (Vector Lab., Burlingame, CA) and 3’,3’-diaminobenzidine (MilliporeSigma ...
-
bioRxiv - Neuroscience 2021Quote: ... goat anti rat (3052-08)) for 1 hour at room temperature and labelled structures were visualized using the ABC Vectastain kit (Vector laboratories, PK-6100). Finally ...
-
bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
-
bioRxiv - Immunology 2022Quote: ... On day 2 secondary antibodies were added for 30 minutes at room temperature and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories #SK-5300). Following this ...
-
bioRxiv - Genomics 2020Quote: ... tissue samples were processed into paraffin blocks and 6-micrometer sections were stained with hematoxylin-eosin and Luxol fast blue and immunostained as described above after antigen retrieval with development using ABC kits from Vector Laboratories (Burlingame, CA).
-
bioRxiv - Molecular Biology 2021Quote: ... dishes were washed with 100 mM tris (pH 8) and AP activity assay was performed using the VECTOR Blue AP Substrate Kit (Vector Laboratories, VECSK-5300) according to the manufacturer’s instructions ...
-
Corticocortical Connections of the Rostral Forelimb Area in Rats: A Quantitative Tract-Tracing StudybioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight in 0.1 M PBS with reagents “A” and “B” added according to Vectastain Elite Kit (Vector Laboratories, Burlingame, CA, #PK6100). Then ...
-
bioRxiv - Developmental Biology 2020Quote: ... was incubated for 1 hour at room temperature followed by a 30-minute incubation with Avidin-Biotin complex (ABC kit, Vector laboratories Cat # PK6100). Finally ...
-
bioRxiv - Neuroscience 2021Quote: Brain tissue sections and cell cultures were evaluated for alkaline phosphatase enzyme activity with the BCIP/NBT substrate kit (SK-5400, Vector Laboratories, Burlingame, CA) as previously described [19,28] ...
-
bioRxiv - Molecular Biology 2022Quote: ... Secondary antibody staining and biotin-streptavidin incubation were performed using species-specific VECTASTAIN Elite ABC kits (Vector Labs, PK-6101 and BMK-2202). DAB Peroxidase Substrate kit (Vector Labs ...
-
bioRxiv - Bioengineering 2022Quote: ... Secondary visualization of the primary antibodies was completed with ImmPRESS Horse anti-rabbit Ig Kit (Catalog No. MP-7401-50, Vector Laboratories, Burlingame, CA) or ImmPRESS Horse anti-mouse Ig Kit (Catalog No ...