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Citations for Vector Labs :
1751 - 1800 of 1890 citations for 6 Cyano imidazo 1 2 a pyridine 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue sections were peroxidase quenched with 3% H2O2 for 15 min at RT and blocked (10% normal goat serum/PBS) for 1 hour at RT before Avidin-Biotin blocking (Vector Laboratories, Inc). Primary antibodies were diluted in 5% serum(FBS)/4% BSA/0.3% Triton-X/PBS and incubated for 30 min at RT (CD31 @ 1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×106 cells were resuspended in 100 µL FACS buffer (1X PBS + 1% bovine serum albumin + 0.05% sodium azide) and stained for 30 mins on ice with the following lectins at 1:400 dilution: SNA-FITC (Vector Laboratories; RRID:AB_2336719), Mal-I-FITC (Bioworld 21761036) ...
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bioRxiv - Neuroscience 2024Quote: ... slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed and blocked for 1 hour at room temperature in 10% normal goat serum (0.1% Triton X in Tris buffer; Vector Labs, Cat# S2000). Both primary and secondary antibodies were diluted in the same Tris buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Cells treated with extensive washes with PBS with 1% BSA prior to flipping the coverslip and mounting on glass slides in anti-fade media (Vector Laboratories, Inc.). The samples were then imaged using confocal fluorescence microscopy (LSM 710 Confocal ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed and incubated with anti-BrdU antibody (1:100) followed by anti-mouse Ig-fluorescein antibody (1:200) and mounted onto a glass slide using DAPI (Vectashield, Vector Laboratories, CA, USA). Images were captured using fluorescence microscope BX53 (Olympus Corporation ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a biotinylated anti-human IgG in TBST with 1% goat serum also incubated for 30 minutes at room temperature (Vector Labs, Burlingame, CA). Then ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti rat (3052-08)) for 1 hour at room temperature and labelled structures were visualized using the ABC Vectastain kit (Vector laboratories, PK-6100). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was rinsed in KPBS-X and reacted with the respective fluorophore-conjugated secondary antibody (1:200, Vector Laboratories Ltd., Peterborough, UK) for 2 h in 0.2% KPBS-X ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed 3 x 10 min in 0.1 M TBS and transferred to a secondary antibody solution with 1:200 anti-mouse IgG (Vector Laboratories, Burlington, ON, Canada) in TBS+ for 4 h at 4ºC ...
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bioRxiv - Neuroscience 2019Quote: ... using respective secondary antibodies (biotinylated donkey anti-rat IgG, donkey anti-mouse IgG, and donkey anti-rabbit IgG (1:500, Vector Laboratories, Burlingame, CA), and Alexa-488 donkey anti-rat IgG ...
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bioRxiv - Developmental Biology 2020Quote: ... was incubated for 1 hour at room temperature followed by a 30-minute incubation with Avidin-Biotin complex (ABC kit, Vector laboratories Cat # PK6100). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... consisting of PBS containing 1% (w/v) Triton X-100 (A1388, AppliChem) and 10% (v/v) normal goat serum (S-1000, Vector Labs, Burlingame, CA) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with Alexa Fluor conjugated anti-mouse and anti-rat antibodies (1:200 dilution) and mounted using the Vectashield without DAPI (Vector Laboratories, H-1000). Images were acquired as described above ...
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bioRxiv - Microbiology 2020Quote: ... The coverslips containing the immuno-stained cells were washed thrice with 1 x PBS then mounted onto glass slides using mounting media with DAPI (Vector Laboratories, Inc., USA). The stained cells were viewed using confocal laser scanning microscopy (CLSM ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... Then the sections were incubated for 24 h (at RT with gentle shaking) with the mixture of Wisteria floribunda agglutinin (WFA) (1:500, Vector Laboratories, B-1355) and mouse anti-Tenascin-R (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... we first detected the DIG-labelled nkx2.4b riboprobe with anti-DIG antibody (1:6000) and BM purple staining in 28 hpf embryos and the DNP-labelled tg riboprobe with anti-DNP antibody (Vector Laboratories, 1:500) and NBT/BCIP (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS (5 × 5 min) and incubated in biotinylated anti-rabbit secondary antibody (1:1000 in PBS with 0.1% bovine serum albumin, Vector Laboratories, Inc., Burlingame, CA) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then reacted with an avidin-biotin conjugate (ABC kit) for 1 hour and visualized using the chromogen VIP (Vectastatin Elite ABC kit and Vector VIP kits; Vector Labs, Burlingame, CA).
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bioRxiv - Neuroscience 2019Quote: ... sections were transferred to a 1:250 dilution of biotinylated horse-anti-mouse IgG (for sections reacted against Crym; Vector Labs, Peterborough, UK) or biotinylated horse-anti-rabbit IgG (for sections reacted against Gng2 ...
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bioRxiv - Neuroscience 2019Quote: ... Following PBS-TX washes the sections were incubated with the secondary antibody (1:200 dilution; goat anti-rabbit DyLight 594; Vector Laboratories; Peterborough, UK), washed in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were then rinsed through another series of 1x PBS washes and incubated in either biotinylated anti-mouse IgG or anti-goat IgG secondary antibody [1:200] (Vector Laboratories, Burlingame, CA). The signal was amplified using a Vectastain ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... in 2% BSA and 0.1 M TBS for 18 h at room temperature followed by incubation with biotinylated goat anti-rabbit Immunoglobin G secondary antibody (1:200; Vector Laboratories, Burlingame, CA) in 2% BSA and 0.1 M TBS for 75 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... was used for blocking the sections for 30 min at room temperature and incubated with Ki-67 antibody (#VP-RM04; Vector Laboratories, 1:100) overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Some sections were also immunostained with the antibody to neuron-specific nuclear protein NeuN (MAB377, 1:2K, Chemicon, Temecula, CA) and visualized using chromogen Vector Red (Vector Laboratories, Burlingame, CA). The stained sections were microphotographed using a 3-megapixel digital microscope camera (Spot RT ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then rinsed with HPLC-grade acetone and immerged into 1% (v/v) Vectabond (product code #SP-1800, Vector Labs, CA, USA) in acetone for 10 min to functionalise the glass surface with amino groups ...
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bioRxiv - Immunology 2019Quote: ... the sections were washed off with PBS and the slides were probed with Biotinylated secondary antibody (Cat No. BA-1000, Vector Laboratories, 1:200) and VECTASTAIN® Elite® ABC HRP reagent (Cat No ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times with 0.1% PBST and then incubated for 1 hour at room temperature with ImmPRESS anti-rabbit peroxidase conjugated antibody (Vector Laboratories, Burlingame, CA, USA). Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100) ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Physiology 2020Quote: ... The slides were then incubated for 1 hour in a humidity chamber in a mixture of goat anti-rabbit peroxidase conjugated (Vector Labs PI-1000). and goat anti-chicken Alexafluor 488 conjugated (Abcam #ab150169) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with DyLight 594-conjugated goat anti-rabbit secondary antibody (1:1000, Vector Laboratories DI-1594) for 2 h on a shaker at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with biotinylated goat anti-rabbit secondary antibody (1:600 dilution; Vector Laboratories, catalog #BA-1000) in PBS-Tx and 1% NGS for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories, Burlingame, CA). Slides were developed with Bio-Red (Biopath ...
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and stained overnight at 4C with FITC-conjugated IB4 (Fluorescein labeled Griffonia Simplicifolia Lectin I isolectin B4, FL-1201, Vector laboratories, 1:200) or primary antibody against Cld-5 (mouse monoclonal ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:200 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500). Imaging was performed on a Leica SP8 Confocal microscope using a 40x oil objective ...
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bioRxiv - Developmental Biology 2022Quote: ... Ki67 staining was performed using monoclonal rabbit anti-Ki67 antibody (1:100, Linaris, Dossenheim, Germany) and signals were amplified by the use of the Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Excess antibody buffer was removed with PBSTx and followed by incubation in biotinylated goat anti-mouse secondary antibody (1:1000 Cat# BP-9200-50: Vector Laboratories, California USA) overnight at 4°C in PBSTx and 1% NGS ...
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bioRxiv - Microbiology 2022Quote: ... brain sections were stained with rabbit anti-SINV capsid antibody (1:5000 dilution) and the Vectastain Elite ABC HRP kit (Vector Laboratories, PK-6101) was used for signal detection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and stained with 1:20 diluted fluorescein-conjugated UEA-I (Lectin Kit I, Fluorescein, UEA-I-FL, #FKL2100; Vector Laboratories, Burlingame, CA, USA). The cells were washed with PBS containing 0.2% FBS and subjected to flow cytometry by a Moxi GO II cell analyzer (Orflo ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody incubation was with horse anti-mouse IgG conjugated to biotin (1:100 in PBS; Vector Labs, catalog BA-2000-1.5) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 × 10 min with PBS and incubated in a tertiary of avidin and biotinylated horseradish peroxidase (1:1000; ABC kit, Vector Labs, PK-6100) and stained with a 3 ...
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bioRxiv - Neuroscience 2022Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Cancer Biology 2022Quote: ... CDX tumors were grown to approximately 750 mm3 and mice received an i.v injection of Biotinylated Tomato Lectin (4mg/kg, Vector Laboratories, B-1175-1) one hour before they were sacrificed by Schedule 1 method ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used for immunohistochemistry included biotinylated goat anti-rabbit (BA-1000) and biotinylated horse anti-goat (BA-9500) at a concentration of 1:200 (Vector laboratories, Burlingame, CA).