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Citations for Vector Labs :
1651 - 1700 of 1912 citations for 6 METHYL 2H PYRIDO 1 2 A PYRIMIDIN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... slides were thoroughly washed and incubated for 1 h with the appropriate secondary antibody: biotinylated anti-rabbit (BA-1000, Vector Laboratories) or biotinylated anti-mouse (BA-2000 ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then washed thrice in phosphate buffered saline prior to 30 min incubation with biotin-conjugated Goat Anti-Mouse IgG (H+L) (1:500, BP-9200, Vector Laboratories). Endogenous peroxidase was blocked and detection was performed using VECTASTAIN® ABC-HRP Kit with signal development carried out using 3,3-diaminobenzidine ...
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bioRxiv - Physiology 2022Quote: ... After reducing non-specific binding with 30 min of Animal-Free Blocker incubation followed by 1 h incubation with Mouse on Mouse Blocking Reagent (Vector Laboratories), sections were incubated with primary antibodies (Proinsulin ...
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bioRxiv - Physiology 2019Quote: ... at a dilution of 1:25 for 20 min using agitation followed by 3 washes with PBT and 1 wash in PBS before mounting in Vectashield with DAPI (#H-1200, Vector Laboratories). Analysis of the tissue and image acquisition was carried out using a Zeiss LSM 710 confocal laserscanning microscope and processed using Fiji and Adobe Photoshop.
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bioRxiv - Molecular Biology 2020Quote: ... cells were stained with 1 µg/ml DAPI and mounted with VECTASHIELD® Antifade Mounting Medium (#H-1000-10, Vector Laboratories). Images were taken using a Perkin Elmer spinning disc confocal equipped with a Nikon-TiE inverted microscope using 60X objective oil lens with an Orca ER CCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Synthetic Biology 2021Quote: ... slides were again washed 5 times for 10 min with 1 × PBS at room temperature and mounted in Vectashield (Vector Laboratories) with 1 µg/ml DAPI ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV cross-reactive N protein 1C7C7 MAb (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was used at 5 μg/mL final concentration for 1 hour at 24°C following by goat anti-rabbit IgG biotin conjugate used as secondary antibody at 1-200 final dilution (BA-1000, Vector Laboratories). DAB Map including 3,3-Diaminobenzidine chromogen was applied to the slides for histological staining.
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with biotinylated anti-goat antibody IgG produced in rabbit (1: 1000; Alexa 594, Vector Laboratories, Burlingame, CA, USA) for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then incubated in ABC (PK 6100, Vectors Laboratories, Burlingame, CA) for 1 hr and oxytocin-ir visualized using Vector-SG (SK-4700; Vector Laboratories). Sections were mounted onto microscope slides and coverslipped ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed five times for ten minutes in PBS and then incubated in an avidin-biotin horseradish peroxidase complex (1:1000; ABC Vector Elite kit; Vector Laboratories) for 4 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... A secondary biotinylated goat-anti rabbit antibody was used (#BA-1000, diluted 1:200 in TBS, Vector Laboratories, Burlingame, CA, USA) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with secondary antibodies for 1 h at RT then washed further in PBS and mounted in Vectashield containing DAPI (Vector Laboratories). Primary antibodies used were ...
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bioRxiv - Developmental Biology 2019Quote: ... Stage 28 hpf embryos were incubated with anti-DIG to detect the nkx2.4b probe and stage 55 hpf embryos were incubated with anti-DNP antibody conjugated to alkaline phosphatase (1:500, Vector Laboratories) to detect tg probe ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was incubated for 40 min in biotinylated secondary anti-rabbit antibody made in goat (1:200; Vector BA-1000) followed by incubation with the avidin–biotin complex solution (Vectastain ABC kit, Vector Laboratories). Immunoreactivity was visualized using standard DAB procedures ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour at room temperature and developed with 3,3’-diaminobenzidine (DAB/ImmPACT DAB) or Vector SG (Vector Laboratories, Burlingame, CA) of for 5-15 minutes (until optimal differentiation of signal) ...
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bioRxiv - Cell Biology 2020Quote: Plasma membranes were labelled by incubating cells with 1:250 wheat germ agglutinin (WGA, v/v) conjugated with the fluorophore FITC or rhodamine (Vector Labs), either live in complete media (immediately after staining to avoid WGA-induced cytotoxicity ...
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bioRxiv - Physiology 2021Quote: ... slides were placed in a blocking buffer (1:60 normal goat serum [Vector Laboratories; Cat. #S-1000] in 0.1% BSA/PBS) for 30 min at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... after washing sections were incubated with biotinylated secondary antibody at room temperature for 1 hour followed by incubation with ABC (Avidin Biotin complex) (Vector Labs) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Slides were then washed with DI water and heated to 90C for 30 minutes in 1% citrate-based antigen unmasking solution (Vector Laboratories) using a Biocare Medical Decloaking Chamber ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibody signal of Cytokeratin 8 was amplified using biotinylated anti-rat antibody (1:300, 0.5 mg, FisherScientific Vector Laboratories, CAS#NC9016344), followed by staining with Streptavidin ...
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bioRxiv - Microbiology 2022Quote: ... for 15 min at room temperature and immunostained using the N protein 1C7C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were rinsed with PBST and incubated with Cy3-conjugated donkey anti-rabbit IgG (1:500, dissolved in blocking solution; Vector Laboratories) secondary antibody for 2 h ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Physiology 2024Quote: ... then rinsed several times in PB followed by incubation for two hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit PK6100, Vector Laboratories). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Physiology 2023Quote: ... Then the sections were treated in avidin/biotin-peroxidase complex (Vectastain Elite ABC Elite 1:500, Vector Laboratories Inc, Burlingame, US) and the GnRH-immunoreactivity was developed in 0.05% DAB /0.005% H2O2 in 0.05 M Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200; Vectastain standard kit, Vector Laboratories, RRID:AB_2336819). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a biotin-conjugated anti-mouse secondary antibody (1:200) and revealed with the DAB peroxidase substrate kit (Vector laboratories) before imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Physiology 2024Quote: ... slides were placed in a blocking buffer (1:60 normal goat serum [Vector Laboratories; Cat. #S-1000] in 0.1% BSA/PBS) for 30 min at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were labeled with DAPI (1:5000 in PBSTx) overnight at 4°C and mounted with Vectasheild (CAT H-1000-10; Vector Laboratories).
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bioRxiv - Microbiology 2024Quote: ... The fixed homogenate was stained with a 1:150 dilution of FITC-conjugated Dolichos biflorus agglutinin (Vector Laboratories cat# FL-1031) in 1X PBS and slowly rotated at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... gondii polyclonal antibody (gifted from Fausto Araujo, Palo Alto Medical Foundation, 1:1000) followed by biotinylated goat anti-rabbit IgG antibody (Vector Laboratories). ABC reagent and DAB substrate (Vector Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Endogenous peroxidase and biotin were blocked with 1% hydrogen peroxide-methanol for 10 min and a biotin blocking kit (Vector Laboratories), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS-T and incubated in blocking solution containing biotinylated goat anti-rabbit secondary antibody (1:250, Vector Laboratories) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea) simplicifolia lectin 1 (5 μg/ml) (Vector Laboratories; L-1100). OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα ...
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bioRxiv - Immunology 2022Quote: ... the sections were washed and counterstained with DAPI for 1 min followed by incubation with autofluorescence quencher (Vector Labs #SP-8400). Sections were finally mounted with Vectashield Vibrance Antifade mounting media ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were washed for three times in TBS and incubated in 10% normal goat serum in TBS-T with a biotinylated secondary antibody from goat against rabbit IgG (1:1000, Vector Laboratories) at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... vWAT sections were incubated overnight at 4°C with primary antibody (CD68, 1:100 in PBST) and visualised by probing with Horse Anti-Rabbit IgG Polymer Kit (MP-7401, Vector Labs) for 1 hour at 37°C and ImmPACT DAB Peroxidase Substrate Kit (SK-4105 ...
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bioRxiv - Genetics 2023Quote: ... fixed in 3% paraformaldehyde for 20 min and permeabilised for 1 h with 0.1% triton-X-100 prior to blocking in 2.5% normal horse serum (Vector Laboratories, Peterborough, UK) for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then washed in 1X PBS and counterstained with Hoescht 33342 (1:2000 in 1X PBS) for 10 minutes All samples were then mounted in Vectashield (Vector Labs) and imaged on with a Nikon A1R laser scanning confocal microscope with appropriate filter sets and laser lines.
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bioRxiv - Cell Biology 2023Quote: Vascular perfusion was assessed by injecting 10 μl of DyLight-649 conjugated Lycopersicon Esculentum (Tomato) lectin (DL-1178-1, Vector Laboratories) intracardially (1mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at room temperature before the coverslips were washed and finally mounted onto glass slides with Vectashield (Vector Laboratories) mounting solution ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in ABC solution (Peroxidase Standard Vectastain Elite ABC-HRP Kit, Vector Laboratories, Catalog# PK-6100). After 2 x 5min washes in 50 mM Tris-HCl ...