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Citations for Vector Labs :
1601 - 1650 of 2388 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and biotinylated LTL (1:300, B-1325-2, Vector Laboratories). Donkey secondary antibodies were used at 1:300 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
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bioRxiv - Neuroscience 2021Quote: ... re-blocked and probed with the relevant primary and secondary antibodies (bound to HRP) then developed with DAB + Nickel (SK4100, Vector Laboratories, UK). Chromogen development reactions were halted with distilled water ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Antibodies were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... and BrdU (mice, AbD Serotec) were detected with biotinylated secondary antibodies (Jackson) and the Vector-Elite ABC kit (Vector Laboratories, Burlingame, CA). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody (ImmPRESS Reagent Kit, MP-7401, Vector Laboratories, Germany) with AEC+ (K346 ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used in the current study were as follows: horseradish peroxidase-conjugated anti-rabbit IgG (PI-1000 [RRID: AB_2336198], Vector Laboratories, goat antibody), Alexa Fluor 568 anti-rabbit IgG (A11036 [RRID ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed four times in PBST and transferred to a secondary antibody solution of goat-anti-rabbit (Vector Laboratories, Peterborough, UK) at a dilution of 1:200 in 1.5% NGS in PBST ...
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bioRxiv - Microbiology 2020Quote: ... and permeabilization in 0.2% Triton (20 min on ice) by sequential incubation with primary and Texas red (TR)-labeled secondary antibodies (Vector Laboratories, Burlingame, Calif.) for 30 min each (all solutions in PBS) ...
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bioRxiv - Physiology 2020Quote: ... sections were blocked for endogenous avidin and biotin activity and incubated with a biotinylated anti-rabbit IgG secondary antibody (0.5%, Vector Laboratories, Burlingame, CA) for 30 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... HIER pre-treated sections were blocked for endogenous alkaline phosphatase activity and non-specific antibody binding using Bloxall (Vector Laboratories, SP-6000) and 2.5% Normal Horse Serum (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with polymer-HRP-conjugated horse anti-goat IgG antibody (ImmPRESS™ HRP Anti-Goat IgG Polymer Detection Kit, Vector Laboratories) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were incubated with the appropriate secondary antibody conjugated with a fluorochrome and mounted with Vectashield® VibranceTM Antifade Mounting medium with DAPI (Vector Laboratories) for nuclei counterstaining ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Anti-mouse monoclonal pS202/pT205 1:1000 (AT8 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody binding and 3,3’-diaminobenzidine staining was performed according to the manufacturer’s instruction (Vectastain Elite ABC Kit, Vector Labs, Peterborough, United Kingdom). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed in PBS (5 x 5) and incubated in biotinylated secondary antibody goat anti-rabbit (Vector Laboratories; BA-100) (1:500 in 1X PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Engineered cartilage sheet sections were also treated with pronase and primary anti-Collagen Type II (DSHB II-II6B3) followed by VectaFluor R.T.U Antibody Kit DyLight® 488 (Vector Labs DI-2788) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Ki67 antibody was detected with an alkaline phosphatase (AP)-conjugated horse anti-rabbit antibody to stain red following incubation with AP substrate (Vector Laboratories, US). After blocking Ki67-stained samples with horse serum ...
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bioRxiv - Immunology 2023Quote: ... was used as a secondary detection for primary antibodies raised in rabbit (CD4 and CD68) and Horse anti-goat IgG polymer reagent (Impress HRP)(Vector Laboratories 30036) for primary antibody MPO raised in goat ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used in the current study were as follows: horseradish peroxidase-conjugated anti-rabbit IgG (Vector Laboratories, PI-1000 [RRID: AB_2336198]); horseradish peroxidase-conjugated anti-mouse IgG (Vector Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... The wells were then incubated in a primary antibody blocking solution containing rabbit anti-GAP45 or biotinylated dolichos (Vector labs B-1035) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG; 1:200 dilution; Vector Laboratories; catalog no. BA-1000) before incubating in an avidin-biotin horseradish peroxidase solution for 2 hrs at room temperature (Vector Novoxastra Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... We then rinsed sections with PB and incubated for 1 h at room temperature in avidin-biotinylated horseradish peroxidase (Vector Laboratories; ABC kit; 1:100). Sections were rinsed ...
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bioRxiv - Neuroscience 2023Quote: ... for the primary and Anti-glutamate receptor 1 (rabbit polyclonal) Goat Anti-rabbit HRP (Novus Biologicals Cat# NB7160, 1:250; Vector Laboratories, Burlingame, CA, USA) for the secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1 h at RT and then stained with EC specific biotinylated isolectin B4 (Vector lab, B-1205-.5, 1:100 dilution) overnight at 4 °C and followed by staining with FITC- streptavidin (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in TBS and incubated for 1 h at RT in an avidin-biotin complex solution (ABC; 1:100; Vector Laboratories, Newark, USA, cat# PK-6100) in TBS ...
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Immunology 2022Quote: ... On day 2 secondary antibodies were added for 30 minutes at room temperature and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories #SK-5300). Following this ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were washed with PBS and incubated with an appropriate secondary antibody followed by avidin-biotin complexes (Vector Laboratories, Burlingame, CA, PK-6100). The antibody reaction was visualized with 3-3’ diaminobenzidine (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The sections treated with biotinylated antibodies were further incubated for one hour in avidin-biotin-peroxidase solution (ABC Elite, Vector laboratories, Burlingame, USA). The protein was finally visualized by a 0.1M PB solution containing 5% 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibody staining and biotin-streptavidin incubation were performed using species-specific VECTASTAIN Elite ABC kits (Vector Labs, PK-6101 and BMK-2202). DAB Peroxidase Substrate kit (Vector Labs ...
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bioRxiv - Immunology 2022Quote: ... sections were incubated using Alexa Fluor-conjugated secondary antibodies (Table S2) and counterstained using VECTASHIELD Antifade Mounting Medium with DAPI (H-1200, Vector Laboratories, Burlingame, CA). After drying for a few hours ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary visualization of the primary antibodies was completed with ImmPRESS Horse anti-rabbit Ig Kit (Catalog No. MP-7401-50, Vector Laboratories, Burlingame, CA) or ImmPRESS Horse anti-mouse Ig Kit (Catalog No ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed four times in PBST and transferred to a secondary antibody solution of goat-anti-rabbit (Dylight Alexa flour 594, Vector Laboratories, Peterborough, UK) or at a dilution of 1:200 at PBST ...
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bioRxiv - Bioengineering 2020Quote: ... After the primary antibody, then treated with biotinlated donkey anti-rat IgG (LifeTech Thermo, A18743) followed by DAB staining using ABC kit (Vector Labs PK-6100) and DAB (Sigma D5637) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed with PBS for 10 minutes 3 times and incubated with a biotinylated secondary antibody followed with avidin– HRP solution (Vectastain ABC HRP Kit; cat #: PK-4000; Vector Laboratories, Burlingame, CA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... The larvae were then washed in PBT and incubated in secondary antibody in PBT for 1hr followed by washing steps and mounted in VectaShield (Vector Laboratories, Burlingame, CA) on slides ...
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bioRxiv - Cancer Biology 2019Quote: ... Reactive sites were identified with biotinylated antimouse and anti-rabbit secondary antibodies and peroxidase ABC reagents (Vector-Elite, Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s instructions and peroxidase activity was visualized with DAB+ reagents (DakoCytomation) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with the appropriate secondary antibody conjugated to a fluorochrome and mounted with Vectashield® Vibrance™ Antifade Mounting medium with DAPI (Vector Laboratories) for nuclei counterstaining ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated first with biotinylated goat anti-rabbit secondary antibody and then with avidin-biotin complex using the Elite Vectastain kit (Vector Laboratories, PK-6101). Chromogen reactions were performed with diaminobenzidine (SIGMAFAST DAB ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sections were then washed and incubated with secondary antibody for 1h at 37°C and developed with ImmPACT Nova RED HRP substrate (Vector Labs, SK-4805). Tissues were counterstained with hematoxylin ...
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bioRxiv - Bioengineering 2024Quote: ... Then sections were incubated for 30 minutes at room temperature with anti-rabbit IgG peroxidase secondary antibodies (ImmPRESS reagent, Vector Laboratories, Burlingame, CA). Afterwards the sections were treated with hydrogen peroxide block (ab94710 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30 min at RT with secondary antibody fluorochrome-conjugated anti-Rabbit Dylight 488 (Vector Laboratories, Newark, CA, USA). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated with biotinylated secondary antibody for 30 minutes followed by ABC kit (VECTASTAIN Elite ABC-HRP kit, Vector Laboratories, PK-6100) for 30 minutes ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... antigens were retrieved on the deparaffinized sections for detection with primary antibodies (Table S1) and further detected with a streptavidin-peroxidase reaction kit and DAB as a chromogen (ABC kit; Vector Labs, Burlingame, CA). To ensure the specificity of the primary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Bound integrins were quantified by an enzyme-linked immunosorbent (ELISA)-like solid-phase assay using biotinylated rabbit anti-velcro (against ACIS/BASE coiled-coil) antibody and HRP-conjugated streptavidin (VECTOR Laboratories #SA-5004). After addition of ABTS ...
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bioRxiv - Immunology 2023Quote: ... The secondary antibodies were washed off with above-mentioned washing buffer for 15 min and finally slides were mounted with DAPI (Vector Laboratories, Burlingame, USA). The stained cells were examined under a Zeiss LSM 780 fluorescence microscope (Carl Zeiss MicroImaging ...