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Citations for Vector Labs :
1501 - 1550 of 1814 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed three times in 0.2% Triton X-100 in 1xPBS for 10 min and incubated with biotinylated goat anti-rabbit (1:1000, Vector Laboratories) for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were placed in 1× PBS with 0.1% Tween-20 with Vectashield and DAPI mounting medium (Vector Lab; H-1200) (1:30 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary antibody solution containing 1:500 biotinylated rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in PBS-T and incubated in blocking solution containing DyLight 488 Streptavidin (1:250, Vector Laboratories) for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... followed by 1 h incubation with ImmPRESS VR anti- rabbit IgG HRP polymer detection kit (MP-6401-15; Vector Laboratories). Chromogenic detection in situ was performed using ImmPACT DAB EqV peroxidase substrate solution (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated with the biotinylated secondary goat anti-rabbit antibody (1:200 BA-1000, Vector Laboratories) 2 hours at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were washed 3 times for 10 minutes each in 1 mL of 1X PBST (0.2% Tween 20 in 1x PBS) and incubated in Vectashield with DAPI (Vector Laboratories) for at least 1 hour before mounting.
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bioRxiv - Neuroscience 2023Quote: ... After incubation for 120min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200 ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with biotinylated Sambucus Nigra Lectin (SNA) in 1% BSA/PBX at pH 6.8 (ZF0207; Vector Laboratories) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were rinsed and incubated for 30 minutes with α-rabbit IgG secondary antibody (1:1000; Vector labs, PK-4001) diluted in blocking solution ...
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bioRxiv - Bioengineering 2024Quote: ... Cells treated with extensive washes with PBS with 1% BSA prior to mounting in anti-fade media (Vector Laboratories, Inc.). The samples were then imaged using confocal microscopy with a LSM 710 laser scanning confocal microscope (Carl Zeiss ...
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bioRxiv - Bioengineering 2024Quote: ... with 0.1% Triton-X and then incubated in the same buffer containing lectin-fluorescein (0.5 mg/ml, FL-1171, Vector Laboratories), overnight for spinal cords and two days for brains ...
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bioRxiv - Neuroscience 2024Quote: ... β-amyloid was detected using thiazine red (1 µM, Cat# 2150-33-6, Chemsavers) associated with DAPI (0.5 µM, Cat# 1485, Vector lab) and a mouse monoclonal antibody against VSM α-smooth muscle actin (Cat# ABT1487 ...
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bioRxiv - Immunology 2024Quote: ... membranes were washed and incubated with an HRP-conjugated anti-rabbit secondary antibody (PI-1000; 1:40,000 dilution; Vector Laboratories). Protein signals were detected on film and quantified using ImageJ software ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol before samples were stained with DAPI (1 µg/ml, 5 min) and mounted in Vectashield (Vector Laboratories). Images were collected with a Leica SP5 II confocal microscope using LAS-AF software (v2.7.3.9723) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein levels were determined by incubating the membrane with ClpL-specific antibodies (1:10,000 in TBS-T + 3% (w/v) bovine serum albumin) and an anti-rabbit alkaline phosphatase conjugate (Vector Laboratories) as the secondary antibody (1:20,000) ...
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bioRxiv - Microbiology 2020Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the N 1C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:3000 in PBS 1X and then mounted with Vectashield antifade medium (cat. H-1000 - Vector Laboratories, Burlingame, CA, USA) and analysed immediately or stored at +4°C until ready for confocal image analysis.
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour at room temperature in 3% normal donkey serum (NDS, Vector Laboratories, Inc., Burlingame, CA, USA) and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Microbiology 2022Quote: ... fixed in 4% paraformaldehyde for 15 minutes then stained with a 1:500 dilution of dolichos bifluorus agglutinin conjugated to FITC in PBS (Vector Labs). FITC-positive cysts were confirmed by fluorescence and morphology under 20x magnification and the total cyst burden per brain was back-calculated.
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in biotinylated secondary antibody diluted 1:500 in block (biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories). Sections were washed in TBS and then incubated for 1 hour at RT in Vectastain kit components A and B ...
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bioRxiv - Cell Biology 2019Quote: ... The slide was then rinsed by ice-cold ethanol/acetic acid (3:1) and mounted in Vectashield with DAPI (Vector Laboratories) to observe the DNA.
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bioRxiv - Neuroscience 2019Quote: ... After staining with Neurotrace 435/455 (1:100 in PBS) and mounting on gelatin-coated slides in Vectashield (catalogue no. H-1400; Vector Laboratories), sections were imaged with a Fluoview FV-1000 confocal imaging system (Olympus ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed (3 × 5 min) in 0.1M PBS at RT, followed by incubation with secondary antibody (biotinylated goat anti-rabbit, (1:400; Vector Laboratories, BA1000) in blocking solution at RT for 1h ...
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bioRxiv - Pathology 2019Quote: ... slides were washed in PBS and the biotinylated rabbit antibody (1:200 dilution, VECTASTAIN® Elite® ABC Kit, Vector Laboratories) was applied in blocking solution for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval was performed in the Biocare Medical LLC decloaking chamber (NC0436641) for 1 hour with citrate-based antigen retrieval buffer (H-3300, Vector Laboratories) and washed with PBS ...
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bioRxiv - Bioengineering 2020Quote: ... sections were washed in PBS and then incubated with a biotinylated goat anti-rabbit secondary antibody (1:200, Vector Laboratories, BA1000) for one hour at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Three drops of normal goat serum in 10ml PBS was used as blocking buffer for 1 hr at room temperature before primary antibodies incubation (Vectastatin ABC Kit; Vector Laboratories). Slides were then incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... To isolate OPC the single cell suspension was sequentially panned on two panning plates coated with anti-BSL 1 Griffonia simplificonia lectin (#L-1100, Vector Labs) and then incubated on a plate coated with anti-CD140a (#135902 ...
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bioRxiv - Neuroscience 2021Quote: Sections used for morphological analyses in Figure 1C were further incubated in a solution of conjugated avidin-biotin horseradish peroxidase (ABC; 1:300; Vector Labs) in TBS (pH = 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were then washed with PBS and mounted using DAPI (1 μg/ml) or VECTASHIELD with DAPI Mounting Medium (Vector Laboratories). Images were captured using a Zeiss 780/880 confocal microscope.
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed and transferred to donkey anti-rabbit secondary antibody (1:500; Jackson Immuno) for 2 hr before detection with ABC (Vectastain ABC Kit, Vector Laboratories) and visualization with DAB substrate (Vector Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were again rinsed in PB and then incubated overnight in biotinylated donkey anti-sheep IgG in PB (1:200, Vector Labs), followed by overnight incubation in streptavidin-conjugated Alexa 488 in PB (1:800 ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 1h incubation of the following primary antibodies diluted in blocking buffer: biotinylated-dolichos biflorus agglutinin (DBA) [1:500] (Vector Laboratories), rabbit-anti SRS9 [1:1000] and mouse-anti SAG1 [1:1000](kindly provided by John Boothroyd ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed 3 times in PBS and the secondary antibody (biotinylated horse anti-rabbit; 1:250, Vector Laboratories, Burlingame, CA) was added and slides were incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... were applied for 1 h at 37°C prior to mounting onto slides using mounting media containing DAPI (Vectashield, Vector Labs). Images were obtained on a Leica SP5 confocal microscope or a Nexcelom Celigo as described in individual figure legends.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The homogenate was blocked using 3% bovine serum albumin (BSA) in 0.2% Triton X-100 before staining with 1:250 rhodamine-conjugated Dolichos biflorus lectin (Vector Laboratories Inc.) to visualize the cyst wall ...
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bioRxiv - Immunology 2021Quote: ... Germany) for 60minutes at a 1:1500 dilution and followed by a biotinylated anti-rat (Cat. BA-5000, Vector Laboratories, Inc.) for 15 minutes at a 1:2000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 1-hour in an avidin biotin complex solution (per kit instructions, ABC Peroxidase, PK-6100, Vector Laboratories) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.1 M PB, for 10 min) to reduce background auto-fluorescence (Clancy & Cauller, 1998) before being mounted in VectaShield (Vector Laboratories) and later imaged using an epifluorescence system (Leica DM 5000 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in appropriate secondary antibody solutions for 2 hours (ChAT (1:200): rabbit anti-goat IgG (biotinylated; Vector Laboratories, BA-5000); PV (1:200) ...
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bioRxiv - Neuroscience 2022Quote: ... staining was enhanced after primary antibody incubation with biotinylated secondary antibodies (biotinylated horse anti-goat IgG, Vector Laboratories: BA-9500, RRID: AB_2336123; 1:300; biotinylated goat anti-rabbit – bGAR, Vector Laboratories: BA-1000 ...
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bioRxiv - Neuroscience 2022Quote: ... all brain sections were transferred into the secondary antibody solution (1:200 in PBS; biotinylated anti-rabbit made in goat, BA-1000 Vector Laboratories) for 60 min at room temperature ...