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Citations for Vector Labs :
1451 - 1500 of 1612 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ImmPRESS® HRP Horse Anti-rabbit (MP-77452) or the Goat Anti-mouse (MP-7401) IgG Polymer kits from Vector Laboratories was used for all IHC staining ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed in PBS 1X three times for 10 min each and then incubated with biotinylated goat anti-mouse IgG or goat anti-rabbit IgG and visualized using an ABC reagent kit (Vector Laboratories) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... followed by staining using ABC/HRP complex (Vectastain ABC kit, peroxidase rat IgG PK-4004, and peroxidase rabbit IgG PK-4001; Vector Laboratories). Stained sections were visualized using 3’3-diaminobenzidine (DAB ...
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bioRxiv - Immunology 2024Quote: ... followed by blocking with avidin and biotin using a commercially available blocking kit per manufacturer’s instructions (Vector Laboratories, Newark, CA, USA). Secondary antibody detection was performed with a horse anti-mouse biotinylated antibody at 1:60 (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated with a secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit/mouse antibody (ImmPRESS HRP Universal PLUS Polymer Kit (Peroxidase, Horse Anti-Rabbit/Mouse IgG)) (Vector Laboratories) for 30 min at RT ...
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bioRxiv - Biochemistry 2024Quote: ... blocking was performed with 2.5% normal horse serum (VECTASTAIN Elite ABC-HRP Kit Mouse IgG, Vector Laboratories Cat. No. PK-6102) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... signal detection and visualization were performed using the Vectastain ABC system followed by NovaRed Substrate Kit (Vector Labs, Newark, CA, USA), counterstained with Hematoxylin and mounted with Clearmount (American Master*Tech Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µm thick sections were cut and stained with hematoxylin and eosin (H&E) or subjected to immunostaining using the Vectastain ABC kit (Vector Laboratories) including antigen retrieval in boiling citrate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody double labelling was through an ImmPress Duet double staining anti-mouse HRP (brown)/anti-rabbit AP (magenta) kit (MP7724, Vector Laboratories, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... unspecific bindings (5% BSA, 5% NGS in PBST, 1h) and Avidin/Biotin (Avidin/Biotin Blocking Kit, Vector Laboratories, SP-2001, 15 min). Samples were incubated overnight at 4°C with anti-MDA antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... and BrdU (mice, AbD Serotec) were detected with biotinylated secondary antibodies (Jackson) and the Vector-Elite ABC kit (Vector Laboratories, Burlingame, CA). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Another PBS/TWEEN washing battery was performed and sections were then incubated in an avidin-biotin-peroxidase complex (ABC kit, Vector Laboratories®) for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody (ImmPRESS Reagent Kit, MP-7401, Vector Laboratories, Germany) with AEC+ (K346 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed again three times in PBS and the 3,3’-diaminobenzidine (DAB) chromogen DAB Peroxidase (HRP) Substrate Kit (Vector Laboratories, Burlingame, CA) prepared immediately prior to use and addition to each slide individually ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
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bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... the sections were immunostained by the avidin–biotin peroxidase method using the Vectastain Elite Kit (PK-6102, Vector Laboratories, Burlingame, CA, USA) with 3–3-diaminobenzidine and hydrogen peroxide as the chromogen ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently incubated with the respective primary and secondary antibodies (Table S3) using an ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) for 60 min at RT ...
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bioRxiv - Physiology 2020Quote: ... Signal amplification was performed using an ABC Kit (Vectastain, #PK6100) for 30 min at RT followed by DAB (Vector Labs, #SK-4100) sub-strate ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by boiling for 20 min in a 0.01 M sodium citrate (pH 6.0) solution and endogenous biotin was blocked using the Avidin/Biotin Blocking Kit (Vector Labs, SP-2001). Slides were incubated overnight with primary antibodies against anti-rabbit H3K4me3 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... Following, the secondary antibody (The ImmPRESS Universal Antibody Kit, anti-mouse/rabbit) and ImmPACT DAB (both purchased from Vector Laboratories, Burlingame, CA) was applied according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Chromogen development was then conducted with HRP 3,3’-Diaminobenzidine (DAB) or Vector Blue kits (Vector Laboratories, Cat # SK-4100 or SK-5300). Chromogen development was halted by submersion in tap water ...
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bioRxiv - Cell Biology 2021Quote: ... before peroxidase staining was conducted for 30 min with a peroxidase staining kit (VECTASTAIN® ABC Reagent, cat # PK-4000, Vector Laboratories) as described by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with polymer-HRP-conjugated horse anti-goat IgG antibody (ImmPRESS™ HRP Anti-Goat IgG Polymer Detection Kit, Vector Laboratories) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and nonspecific epitopes (species-specific serum diluted 1:5 in Tris-buffered saline and 5% bovine serum albumin, or blocking serum from species specific ImmPRESS® kit; Vector Laboratories) and incubated with primary antibody (Table 2 ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and finally an avidin-biotin-peroxidase complex (ABC kit standard, PK-6100, Vectastain, Vector Laboratories, Burlingame, CA, USA; 1:250; 1 h). Peroxidase activity was detected using a solution containing 0.02 % 3,3’-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Neuroscience 2023Quote: ... non-specific binding was blocked for 30 minutes using 2.5% normal horse serum from the ImmPRESS Anti-Mouse IgG / Anti-rabbit IgG Reagent Kit (Vector Laboratories, CA, USA). (iii ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and blocked with 10% goat or rabbit serum in PBS for 60 min and with Avidin/Biotin Blocking Kit (Vector Laboratories, Inc.) for 15 min ...
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bioRxiv - Systems Biology 2024Quote: ... Sections were rinsed once more in 0.1% Triton-X in TBS then incubated in an avidin-biotin complex (PK-6100; Vectastain ABC Elite kit, Vector Laboratories, Newark, CA) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: Slices containing biocytin fills were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer overnight and processed with Vectastain ABC (avidin-biotinylated HRP complex) Elite Kit (Vector Labs, Burlingame, CA). The shape of the slice ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody binding and 3,3’-diaminobenzidine staining was performed according to the manufacturer’s instruction (Vectastain Elite ABC Kit, Vector Labs, Peterborough, United Kingdom). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by blocking of nonspecific binding sites using normal goat serum (10%) and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA). Sections were incubated with phospho-SMAD2/3 (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse βIII Tubulin (Table S2) was revealed using a mouse-on-mouse kit according to manufacturer’s instructions (Vector laboratories, Burlingame, CA).
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bioRxiv - Cell Biology 2022Quote: ... Engineered cartilage sheet sections were also treated with pronase and primary anti-Collagen Type II (DSHB II-II6B3) followed by VectaFluor R.T.U Antibody Kit DyLight® 488 (Vector Labs DI-2788) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG, Vector Laboratories, PK-4004). Antibody binding was visualized with DAB-nickel (VWR ...
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bioRxiv - Physiology 2023Quote: ... Another block was applied with streptavidin and then biotin solutions (Streptavidin/Biotin Blocking Kit, Vector Labs; Newark, CA, USA; Cat. No.: SP2002) at room temperature for 15 min each ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices were incubated with 1% H2O2 to block endogenous peroxidase and then with the appropriate biotinylated secondary antibody using the VECTASTAIN-ABC kit (Vector Laboratories Inc.), following the manufactureŕs instructions ...
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bioRxiv - Neuroscience 2024Quote: ... slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Pathology 2024Quote: ... biotin-labeled goat anti-rabbit IgG or biotin-labeled horse anti-mouse IgG and avidin-biotin-peroxidase complex (VECTASTAIN Elite ABC HRP kit; Vector Laboratories PK6101). Counterstaining by hematoxylin for 30 seconds ...
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bioRxiv - Genetics 2024Quote: ... EdU staining was preformed using a ClickTech EdU Cell Proliferation Kit (BaseClick #BCK-EdU488IM100) according to the manufacturer instructions and mounted with Vectashield containing DAPI (Vector Labs #30326). Samples were imaged with a fully motorized Olympus IX23 microscope with an Olympus DP28 camera ...
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bioRxiv - Immunology 2024Quote: ... Staining using anti-mouse CDH1 (E-cadherin) was revealed using a mouse-on-mouse kit according to manufacturer’s instructions (Vector laboratories, Burlingame, CA). Slides were imaged on a Leica SP8 STED 3X confocal microscope with a 40X 1.3 NA objective ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then blocked with 10% goat serum in PBS-Triton 0.1% (T-PBS) containing 4 drops/ml of Avidin solution (of the Avidin/Biotin Blocking Kit, Vector Labs SP-2001) for 1 hour and then incubated overnight with the primary antibody (Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The incubation with the primary antibody is done for 5 hours followed by biotinylated mouse secondary (Vector Labs, MOM Kit BMK-2202) in 5.75μg/mL ...