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Citations for Vector Labs :
1451 - 1500 of 1535 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with anti-mouse Ig-fluorescein antibody (1:200) and mounted on glass slides using DAPI (Vectashield, Vector Laboratories, CA, USA). Images were acquired in 40X magnification using a fluorescence microscope BX53 (Olympus Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in a solution containing avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories; 1:50) for 2hrs ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... were used as described previously (Kantar et al., 2021) for 1 hour at room temperature before mounting the coverslips with Vectashield (Vector Laboratories #H-1200) and imaging on Zeiss Apotome or Leica Thunder microscopes.
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were blocked with BSA in PBS-Tween and antibodies were incubated at the following dilutions: anti-Biotin (1:1000, Vector Labs, SP-3000), anti-RNA polymerase II CTD repeat YSPTSPS [phospho S5] [4H8] (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Microbiology 2023Quote: ... the slides were incubated with secondary antibodies for 1 hour and washed again 3 times with PBS and mounted with Vectaschield containing DAPI (Vector Laboratories, H-1500).
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times in PBS and 2 times in 0.1 M phosphate buffer solution (10 min/wash) before being mounted and cover-slipped using Vectashield Vibrance w/DAPI (Vector Laboratories H-1800). Fluorescent conjugates of streptavidin were stored and prepared as recommended by the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... The tissues were counterstained with 1 μg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Stained tissues were imaged on confocal Zeiss 710 microscope operated by ZEN software (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at RT and initiation of color reaction with a Peroxidase Substrate Kit (SG Peroxidase Substrate Kit, Vector Laboratories, SK-4700). All stained sections were mounted ...
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bioRxiv - Neuroscience 2023Quote: ... labeled with rabbit anti-ionized calcium-binding adapter molecule (Iba)-1 antibody (Wako Chemicals, Neuss, Germany) and VectaStain Elite ABC-HRP kit (Cat.-No.: PK-6100, Vector Laboratories, Burlingame, USA), and visualized with diaminobenzidine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies with their respective concentrations were as follows Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (1:25, Vector Laboratories, #FL-1201), mouse monoclonal anti-3G5 IgM (1:10 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with primary antibodies overnight at 4°C and with secondary antibodies for 1 hr at room temperature (RT) before ABC-HRP (Vector Laboratories #PK-7100) treatment and DAB (Vector Laboratories #sk-4105 ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:30 was applied for 15 minutes followed by biotinylated secondary rabbit anti-rat antibody diluted 1:400 (BA-4001; Vector Laboratories, Burlingame, CA) for 10 minutes and then ready-to-use Bond polymer refine detection kit with 3,3’- diaminobenzidine (DAB ...
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bioRxiv - Pathology 2024Quote: ... antibodies were coadministered overnight and were labeled with horse anti-rabbit or anti-goat Dylight 488 and 594 (Vector Laboratories, 1:300 dilution) secondary antibodies for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed in PBS 1X and incubated for 45 minutes with goat anti-Rabbit IgG secondary biotinylated antibody (1:500, Vector Laboratories, BA-1000) followed by an incubation with Cy3-conjugated streptavidin (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... at a concentration of 1:200 and a fluorescent-conjugated lectin (WFA) which binds carbohydrate structures and excites at 495 nm (Vector Laboratories, cat. # 1351) at a concentration of 2 ng/mL in blocking solution (2 hr ...
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bioRxiv - Neuroscience 2024Quote: ... The next day sections were incubated at room temperature for one hour with a biotinylated secondary antibody (1:200, Vector Laboratories, Burlingame, CA), washed briefly in PBS ...
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Immunology 2024Quote: ... The tissues were counterstained with 1 µg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Fragments of the lung were fixed at room temperature in 2% PFA in PBS (pH = 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Neuroscience 2019Quote: ... The sections of cerebellum were incubated with monoclonal mouse antibodies against HuCD (ICN, Biomedicals, United States) (1:300) and glutamine synthetase (Vector Laboratories Burlingame, United States) (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 × 5 min in TBS buffer and then incubated for 100 min in a solution of streptavidin Dylight 649 (Vector laboratories, SA5649, 1:100) in TBS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... After washing in 0.1% Triton-X-100 in TBS the sections were incubated for one hour at room temperature with Streptavidin Dy Light Alexa antibody (1:500; Vector Labs, Cat. # SA-5488) in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed in 0.5% TX-100 and incubated for 1.5 hours in anti-mouse biotinylated secondary antibody (1:400; Vector Laboratories; cat # BA-9200; RRID: AB_2336171). Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... The histological sections were washed three times (10 min/each) with PBS, counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, 1:16000) and mounted with Vecta-Shield (H1000, Vector Laboratories, Burlingame, CA, USA) (Varman et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 3% BSA with fluorophore-conjugated lectin added 1:1000 (Vector Labs FL-1111-2). Samples were covered and incubated on ice for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the brain sections were re-stained the Nb-loaded neuron using the avidin-biotin-horseradish peroxidase complex (Vectastain Elite ABC; Vector Laboratories, 1:200 dilution) with diaminobenzidine and nickel as demonstrated previously [23,54] ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Molecular Biology 2021Quote: ... the slides were incubated with a biotin-conjugated secondary antibody for 1 h and with VECTASTAIN ABC Reagent (Cat. #PK- 4000; Vector Laboratories, Burlingame, CA, USA) for 30 min at 20°C ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:200 in 1% PBA and 30 minutes with an ABC mix solution from the VECTASTAIN Elite ABC Reagent kit (Vector Laboratories, PK-6101, RRID:AB_2336820). To reveal the reaction ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling slides in a Coplin jar filled with Citrate-Based Antigen Unmasking Solution (1:100 dilution; Vector Laboratories, Cat# H-3300). After cooling to room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Tadpoles are washed with PBT for 1 hour and then washed in PBS for another hour before mounting in Vectashield (Vector Laboratories, Burlingame, CA, USA). Z-stacks were acquired at 1μm for visualizing epidermal MCC’s and 3μm for visualizing entire brain regions.
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bioRxiv - Neuroscience 2022Quote: ... Signal amplification was completed by incubation with streptavidin conjugated with Alexa-Fluor™-488 (1:500 in 0.1% Triton/TBS; Vector Labs, No. SA-5488). Sections were washed in TBS as described above and mounted using aqueous mounting medium containing DAPI (Fluoroshield ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... we treated the brain sections with the Fluorescein (FITC) labeled Wisteria Floribunda Lectin (WFA, WFL) (1:500 dilution in 1X PBS, Vector labs, cat#FL-1351) for 2 hours at RT before mounting them in clear mounting medium (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...